Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,532	T→C	intergenic (‑114/‑25)	queE ← / → yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,532	0	T	C	100.0%	194.6 / NA	56	intergenic (‑114/‑25)	queE/yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/30);  total (26/30)

CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAAT  >  NC_000913/2905398‑2905653                                                                                                                                        |                                                                                                                          ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTcaata                                                                                                                       >  2:1325870/1‑139 (MQ=255) ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTcaata                                                                                                                       >  1:743800/1‑139 (MQ=255) ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTcaata                                                                                                                       >  2:735220/1‑139 (MQ=255)         tCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                               <  1:958511/139‑1 (MQ=255)            ggTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTcaa                                                                                                                         >  1:440733/1‑126 (MQ=255)             gTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                           <  2:1193254/139‑1 (MQ=255)             gTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                           <  2:1209274/139‑1 (MQ=255)              tACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                 >  2:107890/1‑132 (MQ=255)              tACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                 <  1:107890/132‑1 (MQ=255)               aCTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa                                                                                                         <  2:743800/139‑1 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                           >  2:692093/1‑125 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                           <  1:692093/125‑1 (MQ=255)                              ctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCagaa                                                                                          >  2:406103/1‑139 (MQ=255)                               tgtgAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                                             <  1:1432554/119‑1 (MQ=255)                               tgtgAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                                             >  2:1432554/1‑119 (MQ=255)                                       ggATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                               <  1:754509/109‑1 (MQ=255)                                       ggATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                               >  2:754509/1‑109 (MQ=255)                                       ggATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATaaa                                                                                 <  1:144238/139‑1 (MQ=255)                                           aaTTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                             <  2:1102974/139‑1 (MQ=255)                                                        tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                               <  1:483045/93‑1 (MQ=255)                                                        tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                               >  2:483045/1‑93 (MQ=255)                                                          cAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                 <  1:465480/88‑1 (MQ=255)                                                          cAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                 >  2:465480/1‑88 (MQ=255)                                                             aTCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCa                                                           <  1:735220/139‑1 (MQ=255)                                                                        acaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAAtt                                                                                                                    <  2:460363/71‑1 (MQ=255)                                                                        acaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAAtt                                                                                                                    >  1:460363/1‑71 (MQ=255)                                                                         caaTCATGTCTTATTAACATTCTGTTACGGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAAGTAAAGGGAGTAACGTATTATGt                                                                                               >  2:912293/1‑91 (MQ=255)                                                                         caaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGt                                                                                               <  1:912293/91‑1 (MQ=255)                                                                         caaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                             <  2:382538/109‑1 (MQ=255)                                                                         caaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                             >  1:382538/1‑109 (MQ=255)                                                                         caaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCa                                               >  1:631856/1‑139 (MQ=255)                                                                            tCATGTCTTATTAACATTCTGTTACAGGCAGGCTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGTGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCaaaa                                            >  1:622953/1‑139 (MQ=255)                                                                              aTGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCagaa                                                                                          >  2:307301/1‑91 (MQ=255)                                                                              aTGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCagaa                                                                                          <  1:307301/91‑1 (MQ=255)                                                                                     attaATATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCAc                                                 <  1:1067654/125‑1 (MQ=255)                                                                                     attaACATTCTGTTACACGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCAc                                                 >  2:1067654/1‑125 (MQ=255)                                                                                           aTTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTAtttttt                             <  2:1232553/139‑1 (MQ=255)                                                                                            ttCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTc                            <  1:1325870/139‑1 (MQ=255)                                                                                             tCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa                                                                                                         >  2:1409705/1‑61 (MQ=255)                                                                                             tCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa                                                                                                         <  1:1409705/61‑1 (MQ=255)                                                                                             tCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTa                                                                                                          >  1:1251118/1‑60 (MQ=255)                                                                                             tCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTa                                                                                                          <  2:1251118/60‑1 (MQ=255)                                                                                             tCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGa                                                                        >  2:222247/1‑94 (MQ=255)                                                                                             tCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGa                                                                        <  1:222247/94‑1 (MQ=255)                                                                                               tGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                                             >  2:686132/1‑55 (MQ=255)                                                                                               tGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                                             <  1:686132/55‑1 (MQ=255)                                                                                                gTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTa                                                                                                          >  1:436466/1‑57 (MQ=255)                                                                                                gTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTa                                                                                                          <  2:436466/57‑1 (MQ=255)                                                                                                gTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCAc                                                 >  1:920234/1‑114 (MQ=255)                                                                                                gTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAGCATGTCAAAACATTCAc                                                 <  2:920234/114‑1 (MQ=255)                                                                                                  tACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTAtt                      <  2:631856/139‑1 (MQ=255)                                                                                                    caggcaggTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAGATAAAGAAAATGGATTTAATCATGTCCAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAg                    >  2:892972/1‑139 (MQ=255)                                                                                                           gTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                                            >  2:1003545/1‑76 (MQ=255)                                                                                                           gTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                                            <  1:1003545/76‑1 (MQ=255)                                                                                                           gTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                       >  1:152415/1‑113 (MQ=255)                                                                                                           gTTTAAAG‑AAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                       <  2:152415/113‑1 (MQ=255)                                                                                                                      aaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                       <  1:675562/103‑1 (MQ=255)                                                                                                                      aaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                       >  2:675562/1‑103 (MQ=255)                                                                                                                      aaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGaa   >  2:232810/1‑139 (MQ=255)                                                                                                                       aaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAat  >  1:325009/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                          CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAG‑AAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAAT  >  NC_000913/2905398‑2905653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑