Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,756	C→T	*112* (TAG→TAA)	mazF ←	mRNA interferase toxin, antitoxin is MazE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,756	0	C	T	100.0%	189.4 / NA	59	*112* (TAG→TAA)	mazF	mRNA interferase toxin, antitoxin is MazE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/24);  total (35/24)

GGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGT  >  NC_000913/2910621‑2910888                                                                                                                                        |                                                                                                                                     gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTAc                                                                                                                                   >  1:1199463/1‑139 (MQ=255)      gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAAt                                                                                                                              >  2:392949/1‑139 (MQ=255)            ttCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTAc                                                                                                                        >  2:525461/1‑139 (MQ=255)              ccGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGt                                                                                                                      <  2:122651/139‑1 (MQ=255)                      cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTg                                                                                                              >  2:1157938/1‑139 (MQ=255)                      cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTg                                                                                                              >  1:1407033/1‑139 (MQ=255)                            cgcTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                        <  1:392949/139‑1 (MQ=255)                               tGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                     <  1:525461/139‑1 (MQ=255)                                  ataataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                  <  2:1199463/139‑1 (MQ=255)                                       aGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                             <  1:1157938/139‑1 (MQ=255)                                         tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAAtt                                                                                           >  2:835703/1‑139 (MQ=255)                                           aGCAACCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTATGTCAGGTTGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAAttt                                                                                                                <  1:287756/116‑1 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTAcc                                                                                                                                  <  2:746530/98‑1 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTAcc                                                                                                                                  >  1:746530/1‑98 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAAttt                                                                                                                >  2:287756/1‑116 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAg                                                                                                   >  2:64890/1‑129 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAg                                                                                                   <  1:64890/129‑1 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                         >  2:721625/1‑139 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                         >  1:776023/1‑139 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                         >  2:1282619/1‑139 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                         >  2:1376490/1‑139 (MQ=255)                                           aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                         >  1:389374/1‑139 (MQ=255)                                             cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCctc                                                                                       >  1:615245/1‑139 (MQ=255)                                                aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACg                                                                                                                       <  1:45566/104‑1 (MQ=255)                                                aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACg                                                                                                                       >  2:45566/1‑104 (MQ=255)                                                     cGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                               <  1:1282619/139‑1 (MQ=255)                                                                  tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                  <  2:1172147/107‑1 (MQ=255)                                                                  tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                  >  1:1172147/1‑107 (MQ=255)                                                                       tGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                                                             <  1:1376490/139‑1 (MQ=255)                                                                         tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGct                                                           >  1:579369/1‑139 (MQ=255)                                                                                   tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGccc                                                 >  1:376994/1‑139 (MQ=255)                                                                                     cGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGc                                               >  1:1295401/1‑139 (MQ=255)                                                                                            gTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                        <  2:376994/139‑1 (MQ=255)                                                                                                   ggAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                        >  1:29046/1‑68 (MQ=255)                                                                                                   ggAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                        <  2:29046/68‑1 (MQ=255)                                                                                                   ggAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACtttt                                 >  2:207940/1‑139 (MQ=255)                                                                                                    gAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACttttt                                <  1:207940/139‑1 (MQ=255)                                                                                                       aCCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAcc                             >  2:888419/1‑139 (MQ=255)                                                                                                          tgtgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                          <  2:1407033/139‑1 (MQ=255)                                                                                                           gtgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGAt                         <  1:835703/139‑1 (MQ=255)                                                                                                            tgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCttt                                                                     >  1:647267/1‑94 (MQ=255)                                                                                                            tgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCttt                                                                     <  2:647267/94‑1 (MQ=255)                                                                                                            tgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                        >  1:1426649/1‑139 (MQ=255)                                                                                                              aCCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAg                      <  2:389374/139‑1 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                        <  1:581536/42‑1 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                        >  2:581536/1‑42 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTTCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt       >  2:668287/1‑139 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAc                              <  1:716412/116‑1 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAc                              >  2:716412/1‑116 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCt                    >  1:56488/1‑126 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCt                    <  2:56488/126‑1 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCa        >  2:744270/1‑138 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCa        <  1:744270/138‑1 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt       >  2:634331/1‑139 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt       >  1:431377/1‑139 (MQ=255)                                                                                                                             aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt       >  2:187215/1‑139 (MQ=255)                                                                                                                              gTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc      <  2:1295401/139‑1 (MQ=255)                                                                                                                               ttAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCa     >  2:995668/1‑139 (MQ=255)                                                                                                                                  gTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGt  >  2:546525/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     GGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGT  >  NC_000913/2910621‑2910888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑