Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,979,943 C→T A308V (GCT→GTT) 
*231* (TAG→TAA
lysR →
ygeA ←
transcriptional activator of lysA, autorepressor
Asp/Glu_racemase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,979,9430CT100.0% 124.1 / NA 39A308V (GCT→GTT) 
*231* (TAG→TAA
lysR
ygeA
transcriptional activator of lysA, autorepressor
Asp/Glu_racemase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/19);  total (20/19)

TCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTGCGCC  >  NC_000913/2979826‑2980071
                                                                                                                     |                                                                                                                                
tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCAt                                                                                                                       >  2:554077/1‑129 (MQ=255)
tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCAt                                                                                                                       <  1:554077/129‑1 (MQ=255)
tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACa                                                                                                             >  2:533502/1‑139 (MQ=255)
     cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATc                                                                                                        <  1:432423/139‑1 (MQ=255)
          ccGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCgg                                                                                                   <  1:1302308/139‑1 (MQ=255)
                        gCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                     >  2:839123/1‑139 (MQ=255)
                        gCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                     >  1:386831/1‑139 (MQ=255)
                                                    tAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCt                                                                                                      <  1:1125027/94‑1 (MQ=255)
                                                    tAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCt                                                                                                      >  2:1125027/1‑94 (MQ=255)
                                                              ttACAAGCGGGGTGACCGAAACTAGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCCTCCTTGGCATGGATCGCCGAGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCAc                                               <  1:970478/139‑1 (MQ=255)
                                                                         gTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCaa                                    >  2:603958/1‑139 (MQ=255)
                                                                                  aCTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAAt                                   <  2:869034/131‑1 (MQ=255)
                                                                                  aCTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAAt                                   >  1:1216590/1‑131 (MQ=255)
                                                                                  aCTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAAt                                   <  2:1216590/131‑1 (MQ=255)
                                                                                  aCTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGc                           <  1:603958/139‑1 (MQ=255)
                                                                                  aCTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGATATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAAt                                   >  1:869034/1‑131 (MQ=255)
                                                                                      gTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCa                                                                     >  1:1071707/1‑93 (MQ=255)
                                                                                      gTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCa                                                                     <  2:1071707/93‑1 (MQ=255)
                                                                                        cACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAacac                                                                          >  1:263751/1‑86 (MQ=255)
                                                                                        cACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAacac                                                                          <  2:263751/86‑1 (MQ=255)
                                                                                           ttCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATc                                                                                                        <  1:532585/53‑1 (MQ=255)
                                                                                           ttCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATc                                                                                                        >  2:532585/1‑53 (MQ=255)
                                                                                              tctTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAg                                                                                                            >  2:973822/1‑46 (MQ=255)
                                                                                              tctTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAg                                                                                                            <  1:973822/46‑1 (MQ=255)
                                                                                              tctTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAacac                                                                          >  2:209775/1‑80 (MQ=255)
                                                                                              tctTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAacac                                                                          <  1:209775/80‑1 (MQ=255)
                                                                                              tctTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGa               >  2:835331/1‑139 (MQ=255)
                                                                                                        aTTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                     >  1:773791/1‑59 (MQ=255)
                                                                                                        aTTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                     <  2:773791/59‑1 (MQ=255)
                                                                                                        aTTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTgc     >  1:1145550/1‑139 (MQ=255)
                                                                                                         ttttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTgcg    <  1:600280/139‑1 (MQ=255)
                                                                                                         ttttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTgcg    >  2:600280/1‑139 (MQ=255)
                                                                                                           ttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAACATGACGCCCTGTGCGcc  <  1:839123/139‑1 (MQ=255)
                                                                                                              tcgtcAGTTACGACAGCATAAAAGCGACAGCATCCTCg                                                                                                    >  2:535207/1‑38 (MQ=255)
                                                                                                              tcgtcAGTTACGACAGCATAAAAGCGACAGCATCCTCg                                                                                                    <  1:535207/38‑1 (MQ=255)
                                                                                                              tcgtcAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCCTGGATCGCCGCGGTATCAAac                                                                            >  2:1190490/1‑62 (MQ=255)
                                                                                                              tcgtcAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAac                                                                            <  1:1190490/62‑1 (MQ=255)
                                                                                                                 tcAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAacac                                                                          >  2:287745/1‑61 (MQ=255)
                                                                                                                 tcAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAacac                                                                          <  1:287745/61‑1 (MQ=255)
                                                                                                                     |                                                                                                                                
TCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTGCGCC  >  NC_000913/2979826‑2980071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: