Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,014,287	C→T	*257* (TAG→TAA)	yqeC ←	putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,014,287	0	C	T	100.0%	192.6 / NA	59	*257* (TAG→TAA)	yqeC	putative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/29);  total (30/29)

TCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACG  >  NC_000913/3014155‑3014418                                                                                                                                     |                                                                                                                                    tCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTca                                                                                                                              >  1:183793/1‑139 (MQ=255)           gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg                                                                                                                    >  2:668145/1‑139 (MQ=255)                   caaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                                            <  1:1381176/139‑1 (MQ=255)                     aTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                                          <  1:1134336/139‑1 (MQ=255)                        tGCGCCATACCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                       <  2:131787/139‑1 (MQ=255)                         gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                                                      >  2:144689/1‑139 (MQ=255)                         gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                                                      >  1:439936/1‑139 (MQ=255)                          cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                                                     <  2:408189/139‑1 (MQ=255)                          cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                                                     <  2:439936/139‑1 (MQ=255)                          cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                                                     <  2:790743/139‑1 (MQ=255)                              aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                                                 >  1:1363115/1‑139 (MQ=255)                                aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                                                 >  1:1069557/1‑137 (MQ=255)                                aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                                                 <  2:1069557/137‑1 (MQ=255)                                    aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                  >  1:1469606/1‑116 (MQ=255)                                    aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                  <  2:1469606/116‑1 (MQ=255)                                       ggACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                                        <  1:144689/139‑1 (MQ=255)                                                       cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                                                        >  1:421105/1‑139 (MQ=255)                                                             ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                        >  1:259784/1‑101 (MQ=255)                                                             ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                        <  2:259784/101‑1 (MQ=255)                                                             ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTActt                                                                  >  2:796995/1‑137 (MQ=255)                                                                         tgAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                                                      <  2:660004/139‑1 (MQ=255)                                                                             aaTATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTa                                                                                                                                   <  1:359458/58‑1 (MQ=255)                                                                             aaTATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                        <  2:741714/85‑1 (MQ=255)                                                                             aaTATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                        >  1:741714/1‑85 (MQ=255)                                                                                   cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                  <  1:436911/69‑1 (MQ=255)                                                                                   cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                  >  2:436911/1‑69 (MQ=255)                                                                                   cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                                                      <  1:155813/81‑1 (MQ=255)                                                                                   cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                                                      >  2:155813/1‑81 (MQ=255)                                                                                   cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgt                                                                  >  1:824354/1‑117 (MQ=255)                                                                                   cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgt                                                                  <  2:824354/117‑1 (MQ=255)                                                                                      aCCAACCTCAATGTATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                        <  1:118166/76‑1 (MQ=255)                                                                                      aCCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                        >  2:118166/1‑76 (MQ=255)                                                                                         aaCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                                          >  1:1261151/1‑71 (MQ=255)                                                                                         aaCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                                          <  2:1261151/71‑1 (MQ=255)                                                                                          aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                                                >  2:615146/1‑112 (MQ=255)                                                                                          aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                                                <  1:615146/112‑1 (MQ=255)                                                                                          aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg                                     >  2:117073/1‑139 (MQ=255)                                                                                           ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGc                                    >  1:1174907/1‑139 (MQ=255)                                                                                           ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTATATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGCAGGAGCTCGc                                    >  1:889683/1‑139 (MQ=255)                                                                                               aaTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtg                                >  1:1204982/1‑139 (MQ=255)                                                                                                aTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                       <  2:260074/67‑1 (MQ=255)                                                                                                aTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                       >  1:260074/1‑67 (MQ=255)                                                                                                 tGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtgtg                              <  1:117073/139‑1 (MQ=255)                                                                                                      tatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAAt                          <  2:1363115/136‑1 (MQ=255)                                                                                                      tatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAAt                          <  1:668145/136‑1 (MQ=255)                                                                                                        tcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCg                        >  1:269290/2‑139 (MQ=255)                                                                                                            tCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                  <  2:974367/45‑1 (MQ=255)                                                                                                            tCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                  >  1:974367/1‑45 (MQ=255)                                                                                                             cAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtc                   >  1:829762/1‑139 (MQ=255)                                                                                                              aGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtct                  <  2:421105/139‑1 (MQ=255)                                                                                                                       gAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                       <  2:1235630/45‑1 (MQ=255)                                                                                                                       gAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                       >  1:1235630/1‑45 (MQ=255)                                                                                                                            cccGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCgtgt                                                             <  1:1016698/82‑1 (MQ=255)                                                                                                                            cccGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCgtgt                                                             >  2:1016698/1‑82 (MQ=255)                                                                                                                              cGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACg  >  2:464888/1‑139 (MQ=255)                                                                                                                                 gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGcc                                                                                      >  1:1215814/1‑52 (MQ=255)                                                                                                                                 gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGcc                                                                                      <  2:1215814/52‑1 (MQ=255)                                                                                                                                 gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                                             <  2:617833/61‑1 (MQ=255)                                                                                                                                 gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                                             >  1:617833/1‑61 (MQ=255)                                                                                                                                     |                                                                                                                                    TCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACG  >  NC_000913/3014155‑3014418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑