Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,094,234	T→C	T283A (ACA→GCA)	yggR ←	putative PilT family AAA+ ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,094,234	0	T	C	100.0%	214.0 / NA	61	T283A (ACA→GCA)	yggR	putative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/29);  total (32/29)

CGTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATG  >  NC_000913/3094098‑3094362                                                                                                                                         |                                                                                                                                 cGTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGgtgcgt                                                                                                                                >  2:1337300/1‑139 (MQ=255)  gTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGtt                                                                                                                               >  2:986245/1‑139 (MQ=255)              cTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTc                                                                                                                   >  2:969108/1‑139 (MQ=255)                     cACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAgc                                                                                                            <  1:969108/139‑1 (MQ=255)                       cccGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAgcgc                                                                                                          <  1:367732/139‑1 (MQ=255)                           gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                                      >  1:1070206/1‑139 (MQ=255)                           gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTAAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                                      >  1:871327/1‑139 (MQ=255)                                gATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                                      <  2:353907/134‑1 (MQ=255)                                gATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                                      >  1:353907/1‑134 (MQ=255)                                 aTAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                >  1:475415/1‑139 (MQ=255)                                      tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTa                                                                                                                              <  1:765513/104‑1 (MQ=255)                                      tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTa                                                                                                                              >  2:765513/1‑104 (MQ=255)                                       ctgctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGt                                                                                          <  1:46929/139‑1 (MQ=255)                                                   tATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTcc                                                                              >  2:1279872/1‑139 (MQ=255)                                                    atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCa                                                                             >  2:1169388/1‑139 (MQ=255)                                                           ccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCa                                                                                                        >  2:535359/1‑105 (MQ=255)                                                           ccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCa                                                                                                        <  1:535359/105‑1 (MQ=255)                                                             aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCAGCGCGGGTGCGTTAATCAGCAAt                                                                                                                     >  2:916772/1‑90 (MQ=255)                                                             aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCaac                                                                                                                     <  1:916772/90‑2 (MQ=255)                                                               ctgctgCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                                        >  2:719434/1‑85 (MQ=255)                                                               ctgctgCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                                        <  1:719434/85‑1 (MQ=255)                                                                 gctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAg                                                                >  1:607231/1‑139 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                                    >  1:1183611/1‑101 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                                    <  2:1183611/101‑1 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                >  2:1114925/1‑105 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                <  1:1114925/105‑1 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCa                                                              >  1:658862/1‑139 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCa                                                              <  2:816669/139‑1 (MQ=255)                                                                   tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCa                                                              >  1:286583/1‑139 (MQ=255)                                                                    gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                             <  2:658862/139‑1 (MQ=255)                                                                     cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTcc                                                                              <  1:540347/121‑1 (MQ=255)                                                                     cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTcc                                                                              >  2:540347/1‑121 (MQ=255)                                                                       cGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGc                                                          <  2:607231/139‑1 (MQ=255)                                                                            tGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTa                                                     <  1:1169388/139‑1 (MQ=255)                                                                                   cATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTAcc                                              >  1:318591/1‑139 (MQ=255)                                                                                    aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                                               <  1:169040/105‑1 (MQ=255)                                                                                    aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                                               >  2:169040/1‑105 (MQ=255)                                                                                    aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCt                                             >  2:564193/1‑139 (MQ=255)                                                                                      gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGc                                           <  1:815392/139‑1 (MQ=255)                                                                                      gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGc                                           <  2:318591/139‑1 (MQ=255)                                                                                        ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCGCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                                         >  2:446686/1‑139 (MQ=255)                                                                                        ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                                         <  2:475415/139‑1 (MQ=255)                                                                                        ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                                         <  2:1162292/139‑1 (MQ=255)                                                                                        ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                                         >  2:430104/1‑139 (MQ=255)                                                                                            aCTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTg                                     >  1:307913/1‑139 (MQ=255)                                                                                                    gTTTTCCCTTCGCGAATCAAATTCCGCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCa                             <  1:446686/139‑1 (MQ=255)                                                                                                       ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg                          <  2:424812/139‑1 (MQ=255)                                                                                                       ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg                          <  1:1337300/139‑1 (MQ=255)                                                                                                       ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg                          <  1:564193/139‑1 (MQ=255)                                                                                                              cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                >  2:566120/1‑62 (MQ=255)                                                                                                              cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                <  1:566120/62‑1 (MQ=255)                                                                                                              cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCttt                   >  2:813876/1‑139 (MQ=255)                                                                                                                  aaTCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTcc               >  1:1408342/1‑139 (MQ=255)                                                                                                                    tCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGa                                                                   <  1:903477/85‑1 (MQ=255)                                                                                                                    tCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGa                                                                   >  2:903477/1‑85 (MQ=255)                                                                                                                               aCCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATg  >  1:1396604/1‑139 (MQ=255)                                                                                                                                  gcgGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgc                                                                                                     >  2:1199449/1‑37 (MQ=255)                                                                                                                                  gcgGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgc                                                                                                     <  1:1199449/37‑1 (MQ=255)                                                                                                                                  gcgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                     >  2:725635/1‑53 (MQ=255)                                                                                                                                  gcgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                     <  1:725635/53‑1 (MQ=255)                                                                                                                                   cgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCt                                                                          >  2:962716/1‑63 (MQ=255)                                                                                                                                   cgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCt                                                                          <  1:962716/63‑1 (MQ=255)                                                                                                                                      gtgcgtTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGtt                                                                                         >  1:930035/1‑45 (MQ=255)                                                                                                                                      gtgcgtTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGtt                                                                                         <  2:930035/45‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 CGTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATG  >  NC_000913/3094098‑3094362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑