Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 284,869 C→T P557S (CCG→TCG)  yagF → CP4‑6 prophage, dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913284,8690CT100.0% 136.8 / NA 44P557S (CCG→TCG) yagFCP4‑6 prophage, dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)

CAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCG  >  NC_000913/284740‑285002
                                                                                                                                 |                                                                                                                                     
cAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCa                                                                                                                              >  1:1179331/1‑139 (MQ=255)
       ccTCCGCGCTAAACCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcg                                                                                                                       >  2:1131601/1‑139 (MQ=255)
               cTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACg                                                                                                               <  2:1172313/139‑1 (MQ=255)
                           tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  2:470980/139‑1 (MQ=255)
                           tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  2:252311/139‑1 (MQ=255)
                              tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGTGCGATAACGACATCATCGAGATTg                                                                                                >  1:730613/1‑139 (MQ=255)
                              tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  1:1003694/136‑1 (MQ=255)
                              tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   >  2:1003694/1‑136 (MQ=255)
                              tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTg                                                                                                >  2:503486/1‑139 (MQ=255)
                               ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTGGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAg                                                                                                                            >  1:970890/1‑110 (MQ=255)
                               ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAg                                                                                                                            <  2:970890/110‑1 (MQ=255)
                               ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGc                                                                                               <  1:503486/139‑1 (MQ=255)
                                    aaGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGTGCGATAACGACATCATCGAGATTGCCGTgg                                                                                          <  2:730613/139‑1 (MQ=255)
                                           tgtCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACTTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCt                                                                                   >  2:909478/1‑139 (MQ=255)
                                           tgtCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   >  1:668803/1‑123 (MQ=255)
                                           tgtCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  2:668803/123‑1 (MQ=255)
                                                     caCCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGATTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACt                                                                         >  1:832307/1‑139 (MQ=255)
                                                               cgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGa                                                               >  2:692785/1‑139 (MQ=255)
                                                                        ggCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCg                                                      <  1:609682/139‑1 (MQ=255)
                                                                          cGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                                        >  1:424915/1‑87 (MQ=255)
                                                                          cGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                                        <  2:424915/87‑1 (MQ=255)
                                                                           gtgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCa                                                   <  1:692785/139‑1 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                                        <  2:327329/85‑1 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                                        >  1:327329/1‑85 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   >  1:1320090/1‑90 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  2:822113/90‑1 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  2:1320090/90‑1 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   >  1:822113/1‑90 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCgg                                                     <  1:1449228/136‑1 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCgg                                                     >  2:1449228/1‑136 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGAGTCGATTGGCAAGCTGCGCGATAACGa                                                                                                              <  1:757256/79‑1 (MQ=255)
                                                                            tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGAGTCGATCGGCAAGCTGCGCGATAACGa                                                                                                              >  2:757256/1‑79 (MQ=255)
                                                                              tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACcg                                                >  1:974741/1‑139 (MQ=255)
                                                                                   gggCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   >  2:329705/1‑83 (MQ=255)
                                                                                   gggCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  1:329705/83‑1 (MQ=255)
                                                                                     gcgcCTGATTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACaa                                         <  2:832307/139‑1 (MQ=255)
                                                                                           gCTTCGGCCACGTGTCGCCGGAGGCGCCGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCt                                   <  1:1248937/139‑1 (MQ=255)
                                                                                             ttCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGa                                 <  2:1179331/139‑1 (MQ=255)
                                                                                               cGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTgg                                                                       <  2:675287/99‑1 (MQ=255)
                                                                                               cGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTgg                                                                       >  1:675287/1‑99 (MQ=255)
                                                                                               cGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACg                               <  2:974741/139‑1 (MQ=255)
                                                                                                                    gcTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   >  2:323433/1‑50 (MQ=255)
                                                                                                                    gcTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                                   <  1:323433/50‑1 (MQ=255)
                                                                                                                      tGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGgcgcgttcacgct        >  1:334802/1‑131 (MQ=255)
                                                                                                                           ggcgggtCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGgcgcgttcacgctgccag   <  2:334802/139‑14 (MQ=255)
                                                                                                                            gcgggtCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGcgcg  >  2:123023/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                     
CAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCG  >  NC_000913/284740‑285002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑