Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,129,036	G→A	*255* (TAG→TAA)	glcC →	glycolate‑inducible glc operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,129,036	0	G	A	100.0%	218.9 / NA	67	*255* (TAG→TAA)	glcC	glycolate‑inducible glc operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/36);  total (31/36)

TGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGGCG  >  NC_000913/3128900‑3129168                                                                                                                                         |                                                                                                                                     tGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                    <  1:608496/139‑1 (MQ=255)    aGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGt                                                                                                                                 >  1:502374/1‑139 (MQ=255)        gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                             >  2:59702/1‑139 (MQ=255)        gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                             >  2:894460/1‑139 (MQ=255)               cACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCTTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGc                                                                                                                      <  2:660007/139‑1 (MQ=255)                aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                                     <  2:604277/139‑1 (MQ=255)                aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                                     <  1:859464/139‑1 (MQ=255)                 cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCtt                                                                                                                    <  1:1352596/139‑1 (MQ=255)                      cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCAt                                                                                                               >  2:645533/1‑139 (MQ=255)                          gcgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACaa                                                                                                           <  2:336958/139‑1 (MQ=255)                               cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  1:894460/139‑1 (MQ=255)                                    cgcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                                                 <  1:1165414/139‑1 (MQ=255)                                              tgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                       <  2:1335147/139‑1 (MQ=255)                                                cGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTcc                                                                                     >  1:59029/1‑139 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  1:135626/119‑1 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      >  2:135626/1‑119 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                  >  2:108760/1‑139 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                  >  2:1003486/1‑139 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                  >  1:802144/1‑139 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                  >  1:908611/1‑139 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                  >  2:566204/1‑139 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGC‑TTTCATACAATCGGt                                                                                                      <  2:3314/118‑1 (MQ=255)                                                   aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGC‑TTTCATACAATCGGt                                                                                                      >  1:3314/1‑118 (MQ=255)                                                       tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                                              >  1:517670/1‑139 (MQ=255)                                                       tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                                              >  1:653873/1‑139 (MQ=255)                                                               aaTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                       <  1:387467/122‑1 (MQ=255)                                                               aaTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                       >  2:387467/1‑122 (MQ=255)                                                               aaTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTcc                                                                      <  2:802144/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  2:558379/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  2:1143981/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  2:502374/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  1:1383417/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  1:1436276/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  2:805007/139‑1 (MQ=255)                                                                aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGACTCCGCCCCAATGCGTTCCa                                                                     <  1:59702/139‑1 (MQ=255)                                                                 tctcCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAg                                                                    <  2:1192014/139‑1 (MQ=255)                                                                     cACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                                <  2:755861/139‑1 (MQ=255)                                                                         atatCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                                >  1:829213/1‑135 (MQ=255)                                                                         atatCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                                <  2:829213/135‑1 (MQ=255)                                                                            tCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                                                         <  2:335346/139‑1 (MQ=255)                                                                             cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                                                        >  2:92158/1‑139 (MQ=255)                                                                             cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                                                        <  2:59029/139‑1 (MQ=255)                                                                             cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                                                        >  1:923161/1‑139 (MQ=255)                                                                               aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  2:991303/91‑1 (MQ=255)                                                                               aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      >  1:991303/1‑91 (MQ=255)                                                                               aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                                                      <  1:566204/139‑1 (MQ=255)                                                                               aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                                                      >  2:764618/1‑139 (MQ=255)                                                                                    gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                                 >  1:1347697/1‑139 (MQ=255)                                                                                      aGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGATATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     <  1:1175571/117‑1 (MQ=255)                                                                                      aGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                     >  2:1175571/1‑117 (MQ=255)                                                                                         ccaccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                    >  2:699510/1‑51 (MQ=255)                                                                                             caTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCg                                        <  2:923161/139‑1 (MQ=255)                                                                                               tttGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg                                      <  2:732405/139‑1 (MQ=255)                                                                                                  gATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGAt                                   >  1:439456/1‑139 (MQ=255)                                                                                                       gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                    >  2:990467/1‑37 (MQ=255)                                                                                                        cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTaat                                                                                                   >  2:912680/1‑68 (MQ=255)                                                                                                        cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAAc                                                                                                   <  1:912680/69‑1 (MQ=255)                                                                                                           ggcggTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACAt                          <  2:653873/139‑1 (MQ=255)                                                                                                                  ccGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      >  2:524798/1‑56 (MQ=255)                                                                                                                  ccGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  1:524798/56‑1 (MQ=255)                                                                                                                     cTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTc                                                                                                                 >  1:737533/1‑42 (MQ=255)                                                                                                                     cTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTc                                                                                                                 <  2:737533/42‑1 (MQ=255)                                                                                                                      tGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCAt               <  1:92158/139‑1 (MQ=255)                                                                                                                                   gAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCcggcg  >  2:1308738/1‑139 (MQ=255)                                                                                                                                   gAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCcggcg  >  2:1341552/1‑139 (MQ=255)                                                                                                                                    aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAAt                                                                             >  1:954777/1‑63 (MQ=255)                                                                                                                                    aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAAt                                                                             <  2:954777/63‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGGCG  >  NC_000913/3128900‑3129168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑