Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,296,984 G→A *192* (TAG→TAA yraP → outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,296,9840GA100.0% 76.5 / NA 26*192* (TAG→TAAyraPouter membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAG  >  NC_000913/3296848‑3297115
                                                                                                                                        |                                                                                                                                   
tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                                   <  2:1198272/139‑1 (MQ=255)
   aaGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGTGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcc                                                                                                                                <  1:777960/139‑1 (MQ=255)
        accaccGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                                   <  2:1316340/131‑1 (MQ=255)
                                              gACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                     <  2:576147/139‑1 (MQ=255)
                                                 tGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGcc                                                                                                    <  1:1428194/121‑1 (MQ=255)
                                                 tGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGcc                                                                                                    >  2:1428194/1‑121 (MQ=255)
                                                     cGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACg                                                                              >  2:1397664/1‑139 (MQ=255)
                                                      gTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGa                                                                             >  1:807086/1‑139 (MQ=255)
                                                        gAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATa                                                                           <  2:1124333/139‑1 (MQ=255)
                                                         aaGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGt                                                                                                <  2:939191/117‑1 (MQ=255)
                                                         aaGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATACGACGCTTGCCGCGt                                                                                                >  1:939191/1‑117 (MQ=255)
                                                               aaGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                                    <  1:1057622/139‑1 (MQ=255)
                                                                                    gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAc                                               >  1:22038/1‑139 (MQ=255)
                                                                                     ccGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                                   <  2:246852/54‑1 (MQ=255)
                                                                                     ccGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                                   >  1:246852/1‑54 (MQ=255)
                                                                                                 cgTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGATGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACa                                  >  1:929414/1‑139 (MQ=255)
                                                                                                    gAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                                 <  1:642293/89‑1 (MQ=255)
                                                                                                    gAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                                 >  2:642293/1‑89 (MQ=255)
                                                                                                           gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCt                        <  2:22038/139‑1 (MQ=255)
                                                                                                                    gCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                                    >  2:952357/1‑86 (MQ=255)
                                                                                                                    gCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                                    <  1:952357/86‑1 (MQ=255)
                                                                                                                     cgtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTTATGCGACGCTTGcc                                                                                                    <  2:893466/53‑1 (MQ=255)
                                                                                                                     cgtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGcc                                                                                                    >  1:893466/1‑53 (MQ=255)
                                                                                                                         tacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTc                       >  2:223415/1‑126 (MQ=255)
                                                                                                                         tacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTc                       <  1:223415/126‑1 (MQ=255)
                                                                                                                               tattaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                           >  1:900205/1‑52 (MQ=255)
                                                                                                                               tattaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                           <  2:900205/52‑1 (MQ=255)
                                                                                                                                 ttaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAAcc                                                                     <  2:1079370/72‑1 (MQ=255)
                                                                                                                                 ttaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAAcc                                                                     >  1:1079370/1‑72 (MQ=255)
                                                                                                                                 ttaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAg  >  1:1356903/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                   
TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAG  >  NC_000913/3296848‑3297115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: