Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,299,438 A→G T155T (ACA→ACG)  yhbO → stress‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,299,4380AG98.0% 173.4 / ‑4.6 51T155T (ACA→ACG) yhbOstress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (28/22);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGA  >  NC_000913/3299314‑3299563
                                                                                                                            |                                                                                                                             
aTGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGcc                                                                                                                 >  2:570111/1‑139 (MQ=255)
          ggggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            <  2:869602/118‑1 (MQ=255)
          ggggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTAGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            >  1:869602/1‑118 (MQ=255)
              cgcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACcgcg                                                                                                   >  1:147664/1‑139 (MQ=255)
               gcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            <  1:114622/113‑1 (MQ=255)
               gcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            >  2:114622/1‑113 (MQ=255)
                       aCCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCAGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTAc                                                                                          >  1:45523/1‑139 (MQ=255)
                        ccGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACg                                                                                         >  1:1249581/1‑139 (MQ=255)
                               tAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  <  1:1067632/139‑1 (MQ=255)
                                      aTCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCt                                                                           >  2:214959/1‑139 (MQ=255)
                                                aTGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTa                                                                 >  1:170643/1‑139 (MQ=255)
                                                  gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                               >  1:632689/1‑139 (MQ=255)
                                                  gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                               >  1:251144/1‑139 (MQ=255)
                                                     aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGcc                                                                                       >  1:94026/1‑112 (MQ=255)
                                                     aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGcc                                                                                       <  2:94026/112‑1 (MQ=255)
                                                     aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCgg                                                                                >  1:183403/1‑119 (MQ=255)
                                                     aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCgg                                                                                <  2:183403/119‑1 (MQ=255)
                                                        aaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATc                                                         <  2:1249581/139‑1 (MQ=255)
                                                             gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                  <  2:915361/93‑1 (MQ=255)
                                                             gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                  >  1:915361/1‑93 (MQ=255)
                                                             gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  >  1:1417555/1‑109 (MQ=255)
                                                             gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  <  2:1417555/109‑1 (MQ=255)
                                                                  cgGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                             >  1:703017/1‑93 (MQ=255)
                                                                  cgGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                             <  2:703017/93‑1 (MQ=255)
                                                                        tttACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGc                                         <  2:170643/139‑1 (MQ=255)
                                                                               tCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGc                                                                                                              >  1:17099/1‑63 (MQ=255)
                                                                               tCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGc                                                                                                              <  2:17099/63‑1 (MQ=255)
                                                                               tCAGGAAGTCGTGGTTGATAAAGATCAGCTGATTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCgt                                  >  2:13801/1‑139 (MQ=255)
                                                                                       tCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCgg                          <  2:147664/139‑1 (MQ=255)
                                                                                         gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            >  1:389195/1‑39 (MQ=38)
                                                                                         gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            <  2:389195/39‑1 (MQ=255)
                                                                                           ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  <  2:1146754/79‑1 (MQ=255)
                                                                                           ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  >  1:1146754/1‑79 (MQ=255)
                                                                                           ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGaa                      >  2:281707/1‑139 (MQ=255)
                                                                                                aTAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGa                 >  2:1029969/1‑139 (MQ=255)
                                                                                                 tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGAt                >  2:1183935/1‑139 (MQ=255)
                                                                                                      aTCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc                                                                                                                  >  2:1034548/1‑36 (MQ=255)
                                                                                                      aTCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc                                                                                                                  <  1:1034548/36‑1 (MQ=37)
                                                                                                      aTCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  >  2:936774/1‑68 (MQ=255)
                                                                                                      aTCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  <  1:936774/68‑1 (MQ=255)
                                                                                                       tCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGc                                                                                        <  2:1216979/61‑1 (MQ=255)
                                                                                                       tCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGc                                                                                        >  1:1216979/1‑61 (MQ=255)
                                                                                                         aGCTGGTTACCAGCCGGACGCCGGACGATCTGCCATCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAATTCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAtt                    <  2:820955/127‑1 (MQ=255)
                                                                                                         aGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAtt                    >  1:820955/1‑127 (MQ=255)
                                                                                                          gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg       <  1:214959/139‑1 (MQ=255)
                                                                                                           cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                             >  1:802192/1‑52 (MQ=255)
                                                                                                           cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                             <  2:802192/52‑1 (MQ=255)
                                                                                                           cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  <  2:104575/63‑1 (MQ=255)
                                                                                                           cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  >  1:104575/1‑63 (MQ=255)
                                                                                                              gTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCgg   >  1:1118306/1‑139 (MQ=255)
                                                                                                               ttACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGa  <  1:1183935/139‑1 (MQ=255)
                                                                                                                            |                                                                                                                             
ATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGA  >  NC_000913/3299314‑3299563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑