Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,299,999 G→A A12T (GCC→ACC)  yhbQ → GIY‑YIG nuclease superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,299,9990GA100.0% 173.1 / NA 53A12T (GCC→ACC) yhbQGIY‑YIG nuclease superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/22);  total (31/22)

AACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGAT  >  NC_000913/3299868‑3300133
                                                                                                                                   |                                                                                                                                      
aacaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGGTTCCATTGCTGTTCTCTTTTATACTGTCGGCCTGACCTTAACATGCGACGCAGACGATTATACTCTGGTTTCTTTACTTGATTCGTACCACCGACat                                                                                                                                 >  1:899804/1‑138 (MQ=255)
  caTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATa                                                                                                                               <  2:521829/139‑1 (MQ=255)
  caTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATa                                                                                                                               >  1:767505/1‑139 (MQ=255)
     gttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGc                                                                                                                            >  2:447516/1‑139 (MQ=255)
        gttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCttt                                                                                                                         >  1:1141231/1‑139 (MQ=255)
         ttttGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCttt                                                                                                                         <  2:852327/138‑1 (MQ=255)
         ttttGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCttt                                                                                                                         >  1:852327/1‑138 (MQ=255)
             gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                           <  1:1111430/132‑1 (MQ=255)
             gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                           >  2:1111430/1‑132 (MQ=255)
             gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc                                                                                                                    >  1:431276/1‑139 (MQ=255)
               cagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCgg                                                                                                                  >  2:125099/1‑139 (MQ=255)
                   caACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATc                                                                                                              <  2:767505/139‑1 (MQ=255)
                          tGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACTCCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                       <  1:125099/139‑1 (MQ=255)
                                  aTGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                               <  1:727081/139‑1 (MQ=255)
                                                 gCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                           >  2:763970/1‑96 (MQ=255)
                                                 gCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                           <  1:763970/96‑1 (MQ=255)
                                                  cTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCagcagc                                                                               >  1:1124318/1‑139 (MQ=255)
                                                       ctctTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                 <  1:1466393/100‑1 (MQ=255)
                                                       ctctTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                 >  2:1466393/1‑100 (MQ=255)
                                                                  tgtgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                               >  2:110187/1‑139 (MQ=255)
                                                                    tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAc                                                                                                                     >  2:499794/1‑83 (MQ=255)
                                                                    tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAc                                                                                                                     <  1:499794/83‑1 (MQ=255)
                                                                    tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                             >  2:453459/1‑139 (MQ=255)
                                                                    tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                             >  2:137900/1‑139 (MQ=255)
                                                                    tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                             >  2:743753/1‑139 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                               >  2:1431276/1‑135 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                               <  1:1431276/135‑1 (MQ=255)
                                                                      ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCg                                                           >  1:90816/1‑139 (MQ=255)
                                                                               ttAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTg                                                  <  2:1124318/139‑1 (MQ=255)
                                                                                  aCATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTaccacc                                                                                                                                      >  1:60834/1‑52 (MQ=255)
                                                                                      gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGa                                           >  2:1143222/1‑139 (MQ=255)
                                                                                      gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGa                                           >  2:1294308/1‑139 (MQ=255)
                                                                                      gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGa                                           >  1:1342294/1‑139 (MQ=255)
                                                                                        gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaaa                                         <  1:110187/139‑1 (MQ=255)
                                                                                        gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaaa                                         <  1:743753/139‑1 (MQ=255)
                                                                                             aGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATaa                                                                                                                              <  2:576757/49‑1 (MQ=255)
                                                                                             aGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATaa                                                                                                                              >  1:576757/1‑49 (MQ=255)
                                                                                               aCGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGa                                                                                                <  1:590249/77‑1 (MQ=255)
                                                                                               aCGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGa                                                                                                >  2:590249/1‑77 (MQ=255)
                                                                                               aCGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAAc                                  <  2:431276/139‑1 (MQ=255)
                                                                                                    gACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACg                             <  1:447516/139‑1 (MQ=255)
                                                                                                         ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                                          >  1:1017700/1‑57 (MQ=255)
                                                                                                         ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                                          <  2:1017700/57‑1 (MQ=255)
                                                                                                         ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt                        >  1:651669/1‑139 (MQ=255)
                                                                                                         ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt                        >  1:1424124/1‑139 (MQ=255)
                                                                                                                 cTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCcg                <  1:1143222/139‑1 (MQ=255)
                                                                                                                  tttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCcgc               <  2:1424124/139‑1 (MQ=255)
                                                                                                                  tttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCcgc               >  1:232637/1‑139 (MQ=255)
                                                                                                                   ttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCcgcg              >  2:830033/1‑139 (MQ=255)
                                                                                                                    tACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCgcgc             <  2:651669/139‑1 (MQ=255)
                                                                                                                           tCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTggg      >  2:428151/1‑139 (MQ=255)
                                                                                                                               tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                             >  2:163816/1‑48 (MQ=255)
                                                                                                                               tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                             <  1:163816/48‑1 (MQ=255)
                                                                                                                               tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGAt  >  1:364120/1‑139 (MQ=255)
                                                                                                                               tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGAt  >  1:154236/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGAT  >  NC_000913/3299868‑3300133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑