Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,515,726	T→C	Y63C (TAT→TGT)	dam ←	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,515,726	0	T	C	100.0%	219.1 / NA	61	Y63C (TAT→TGT)	dam	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/33);  total (28/33)

CCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATA  >  NC_000913/3515593‑3515854                                                                                                                                      |                                                                                                                                 ccGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAgaga                                                                                                                             <  2:544881/139‑1 (MQ=255)  cGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGAc                                                                                                                            <  2:1263023/139‑1 (MQ=255)        aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCa                                                                                                                      >  2:429728/1‑139 (MQ=255)          cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAgg                                                                                                                    >  1:987083/1‑139 (MQ=255)           ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGt                                                                                                                   <  1:479707/139‑1 (MQ=255)                 tGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTAt                                                                                                             <  2:211992/139‑1 (MQ=255)                      tttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGata                                                                                                        <  1:585754/139‑1 (MQ=255)                       ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGatat                                                                                                       >  1:521834/1‑139 (MQ=255)                          ttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCgg                                                                                                    <  1:303011/139‑1 (MQ=255)                            gAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCa                                                                                                  <  2:1433656/139‑1 (MQ=255)                            gAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCa                                                                                                  <  2:133739/139‑1 (MQ=255)                                 ctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGat                                                                                             <  1:1180623/139‑1 (MQ=255)                                  ttCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGata                                                                                            >  2:1325804/1‑139 (MQ=255)                                              aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGAt                                                                                                                        <  1:1209397/99‑1 (MQ=255)                                              aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGAt                                                                                                                        >  2:1209397/1‑99 (MQ=255)                                              aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGaaaaa                                                                                >  2:374905/1‑138 (MQ=255)                                               tAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                               <  2:987083/139‑1 (MQ=255)                                                    aaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                          <  1:429728/139‑1 (MQ=255)                                                        tCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGt                                                                                                                   >  1:785637/1‑94 (MQ=255)                                                        tCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGt                                                                                                                   <  2:785637/94‑1 (MQ=255)                                                         cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGc                                                                                                                <  1:464415/96‑1 (MQ=255)                                                         cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGc                                                                                                                >  2:464415/1‑96 (MQ=255)                                                          ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGt                                                                                                                   >  1:729993/1‑92 (MQ=255)                                                          ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGt                                                                                                                   <  2:729993/92‑1 (MQ=255)                                                          ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgaga                                                                    >  1:1246078/1‑139 (MQ=255)                                                             gcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGG‑‑ATAACGAGAAAAGTCGGTGTTGAGAAacac                                                               <  2:232352/139‑1 (MQ=255)                                                                          ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                    <  2:1246078/139‑1 (MQ=255)                                                                           gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCa                                                                                                                      >  1:1236425/1‑72 (MQ=255)                                                                           gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCa                                                                                                                      <  2:1236425/72‑1 (MQ=255)                                                                                     cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGc                                         <  1:1429241/139‑1 (MQ=255)                                                                                     cTCGCGTGCGCCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCTGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGc                                         <  1:374905/139‑1 (MQ=255)                                                                                         cgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                        <  2:1017877/104‑1 (MQ=255)                                                                                         cgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                        >  1:1017877/1‑104 (MQ=255)                                                                                                  tGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAtt                            <  2:521834/139‑1 (MQ=255)                                                                                                          cTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTg                    >  2:501374/1‑139 (MQ=255)                                                                                                            catcaGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                  >  2:793363/1‑139 (MQ=255)                                                                                                             atcaGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                 >  1:115719/1‑139 (MQ=255)                                                                                                             atcaGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                 >  1:315768/1‑139 (MQ=255)                                                                                                              tcaGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCa                                                                                                  >  1:1030701/1‑57 (MQ=255)                                                                                                              tcaGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCa                                                                                                  <  2:1030701/57‑1 (MQ=255)                                                                                                               caGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa               >  2:1219859/1‑139 (MQ=255)                                                                                                                  tACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCt                      <  1:353217/129‑1 (MQ=255)                                                                                                                  tACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCt                      >  2:353217/1‑129 (MQ=255)                                                                                                                  tACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg            >  1:481295/1‑139 (MQ=255)                                                                                                                  tACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg            >  2:760104/1‑139 (MQ=255)                                                                                                                  tACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg            <  1:760104/139‑1 (MQ=255)                                                                                                                     gCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg         <  1:501374/139‑1 (MQ=255)                                                                                                                      cATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt        <  1:1083733/139‑1 (MQ=255)                                                                                                                       aTCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg            >  1:1307367/1‑134 (MQ=255)                                                                                                                       aTCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg            <  2:1307367/134‑1 (MQ=255)                                                                                                                       aTCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt       <  2:315768/139‑1 (MQ=255)                                                                                                                       aTCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt       >  2:747635/1‑139 (MQ=255)                                                                                                                            cacaATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAtt                            <  2:144533/113‑1 (MQ=255)                                                                                                                            cacaATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAtt                            >  1:144533/1‑113 (MQ=255)                                                                                                                            cacaATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAata  >  1:12069/1‑139 (MQ=255)                                                                                                                                 tGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                               >  2:533864/1‑57 (MQ=255)                                                                                                                                 tGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                               <  1:533864/57‑1 (MQ=255)                                                                                                                                 tGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                          >  2:78499/1‑62 (MQ=255)                                                                                                                                 tGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                          <  1:78499/62‑1 (MQ=255)                                                                                                                                 tGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAtt                            >  2:436265/1‑108 (MQ=255)                                                                                                                                 tGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAtt                            <  1:436265/108‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 CCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATA  >  NC_000913/3515593‑3515854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑