Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,569,410	T→C	D637G (GAT→GGT)	glgX ←	glycogen debranching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,569,410	0	T	C	100.0%	163.5 / NA	46	D637G (GAT→GGT)	glgX	glycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/17);  total (29/17)

TGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTG  >  NC_000913/3569285‑3569543                                                                                                                              |                                                                                                                                      tGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGaa                                                                                                                          <  1:84140/139‑1 (MQ=255)                   tGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCa                                                                                                        >  2:194582/1‑139 (MQ=255)                       cGTTCTTCTCTAAACTAACCATGACTAACTCTTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTcc                                                                                                    >  2:237471/1‑139 (MQ=255)                       cGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc                                                                                                          >  1:792644/1‑133 (MQ=255)                       cGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc                                                                                                          <  2:792644/133‑1 (MQ=255)                       cGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTcc                                                                                                    >  1:99652/1‑139 (MQ=255)                                         ccATAACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCAGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCAGTCCCCAGCAGGTAAAACAAtc                                                                                  >  1:855023/1‑139 (MQ=255)                                             gACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTg                                                                              >  2:1368078/1‑139 (MQ=255)                                             gACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTg                                                                              >  2:867970/1‑139 (MQ=255)                                                  aCTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAg                                                                                                                              <  2:17977/86‑1 (MQ=255)                                                  aCTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAg                                                                                                                              >  1:17977/1‑86 (MQ=255)                                                     ccTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTc                                                                                                     >  2:415359/1‑108 (MQ=255)                                                     ccTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTc                                                                                                     <  1:415359/108‑1 (MQ=255)                                                          tttATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGt                                                                 <  2:855023/139‑1 (MQ=255)                                                                atcTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATggggg                                                                                                                    <  1:1001148/82‑1 (MQ=255)                                                                atcTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATggggg                                                                                                                    >  2:1001148/1‑82 (MQ=255)                                                                atcTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGtt                                                           >  1:869609/1‑139 (MQ=255)                                                                       gAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCg                                                    <  2:99652/139‑1 (MQ=255)                                                                            cacacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTg                                                      >  1:599724/1‑132 (MQ=255)                                                                            cacacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTg                                                      <  2:599724/132‑1 (MQ=255)                                                                            cacacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCaa                                               >  2:1130759/1‑139 (MQ=255)                                                                             acacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCaaa                                              >  2:674069/1‑139 (MQ=255)                                                                                 aaTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGc                                          >  1:21151/1‑139 (MQ=255)                                                                                  aTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCg                                         >  2:439958/1‑139 (MQ=255)                                                                                   tCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGa                                        <  2:869609/139‑1 (MQ=255)                                                                                       tgtgCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCg                                    >  2:1436268/1‑139 (MQ=255)                                                                                       tgtgCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGAGTTAATTGCGATCAAAAAGCGATCCg                                    >  1:1231653/1‑139 (MQ=255)                                                                                         tgCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCgag                                  >  2:1000966/1‑139 (MQ=255)                                                                                           cAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCTGagag                                >  2:821773/1‑139 (MQ=255)                                                                                              gTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc                                                                                                          <  1:1205342/62‑1 (MQ=255)                                                                                              gTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc                                                                                                          >  2:1205342/1‑62 (MQ=255)                                                                                              gTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAg                             >  1:320347/1‑139 (MQ=255)                                                                                                 ccTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGt                                                                             >  2:1467482/1‑88 (MQ=255)                                                                                                 ccTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGt                                                                             <  1:1467482/88‑1 (MQ=255)                                                                                                   tGCCAGACAGCCGTAATCGCTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACaa                                                                                    >  2:968086/1‑79 (MQ=255)                                                                                                   tGCCAGACAGCCGTAATCACTGGGATACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACaa                                                                                    <  1:968086/79‑1 (MQ=255)                                                                                                        gACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc                                                                                                          >  1:978025/1‑52 (MQ=255)                                                                                                        gACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc                                                                                                          <  2:978025/52‑1 (MQ=255)                                                                                                             ccGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGttt              >  2:1150347/1‑139 (MQ=255)                                                                                                               gTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCg                                                                                                             >  2:709802/1‑42 (MQ=255)                                                                                                               gTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCg                                                                                                             <  1:709802/42‑1 (MQ=255)                                                                                                                  aTCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGcc         <  1:237471/139‑1 (MQ=255)                                                                                                                       tGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGttt    <  2:21151/139‑1 (MQ=255)                                                                                                                         ggTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTg  >  2:1184771/1‑139 (MQ=255)                                                                                                                          gTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGttt    >  1:939602/1‑136 (MQ=255)                                                                                                                          gTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGttt    <  2:939602/136‑1 (MQ=255)                                                                                                                              |                                                                                                                                      TGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTG  >  NC_000913/3569285‑3569543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑