Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,598,557 A→G *368Q (TAA→CAA)  livJ ← branched‑chain amino acid ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,598,5570AG100.0% 209.8 / NA 58*368Q (TAA→CAA) livJbranched‑chain amino acid ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/28);  total (30/28)

CTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAT  >  NC_000913/3598422‑3598692
                                                                                                                                       |                                                                                                                                       
cTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCtt                                                                                                                                      >  2:364687/1‑139 (MQ=255)
  aCAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCg                                                                                                                                    >  1:387993/1‑139 (MQ=255)
   cAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGc                                                                                                                                   >  1:1229438/1‑139 (MQ=255)
   cAATGTGTGTTGCAATTTACTGATTCCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGc                                                                                                                                   >  2:1199652/1‑139 (MQ=255)
        tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCgg                                                                                                                              >  1:1328637/1‑139 (MQ=255)
             tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCgcc                                                                                                                         >  1:456268/1‑139 (MQ=255)
                    tACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCgc                                                                                                                          >  1:85479/1‑131 (MQ=255)
                    tACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCgc                                                                                                                          <  2:85479/131‑1 (MQ=255)
                         aTTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCg                                                                                                             <  1:466773/139‑1 (MQ=255)
                          tttCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGt                                                                                                            >  2:1385047/1‑139 (MQ=255)
                           ttCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTg                                                                                                           <  1:518499/139‑1 (MQ=255)
                            tCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGc                                                                                                          <  2:785857/139‑1 (MQ=255)
                                   aTCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCg                                                                                                                                    >  2:287809/1‑106 (MQ=255)
                                   aTCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCg                                                                                                                                    <  1:287809/106‑1 (MQ=255)
                                       tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATAc                                                                                               >  1:1142557/1‑139 (MQ=255)
                                       tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATAc                                                                                               >  1:1126321/1‑139 (MQ=255)
                                        gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCg                                                                                                             <  2:549273/124‑1 (MQ=255)
                                        gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCg                                                                                                             >  1:549273/1‑124 (MQ=255)
                                        gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACg                                                                                              >  2:793766/1‑139 (MQ=255)
                                              cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAAc                                                                                        >  1:207565/1‑139 (MQ=255)
                                                gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTc                                                                                      >  2:1325171/1‑139 (MQ=255)
                                                  tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaa                                                                                    <  2:1365329/139‑1 (MQ=255)
                                                   aaGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                                   <  2:969781/139‑1 (MQ=255)
                                                   aaGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                                   <  1:1385047/139‑1 (MQ=255)
                                                   aaGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                                   <  2:1142557/139‑1 (MQ=255)
                                                             cgccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGa                                                                         <  1:364687/139‑1 (MQ=255)
                                                                cgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCg                                                                      >  2:1120831/1‑139 (MQ=255)
                                                                  cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                    >  1:822261/1‑139 (MQ=255)
                                                                  cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                    <  2:387993/139‑1 (MQ=255)
                                                                  cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                    <  1:1120831/139‑1 (MQ=255)
                                                                                   cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCa                                                   >  1:193338/1‑139 (MQ=255)
                                                                                    gTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                  <  2:1126321/139‑1 (MQ=255)
                                                                                     tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                  >  1:841277/1‑138 (MQ=255)
                                                                                     tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                  <  2:841277/138‑1 (MQ=255)
                                                                                     tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                                 <  2:822261/139‑1 (MQ=255)
                                                                                        tCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCgc                                                                                                                          >  1:587822/1‑63 (MQ=255)
                                                                                        tCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCgc                                                                                                                          <  2:587822/63‑1 (MQ=255)
                                                                                         cGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGA‑TGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTcc                                            >  2:1021943/1‑139 (MQ=255)
                                                                                              ttAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGt                                                                                                                             <  1:1094449/54‑1 (MQ=255)
                                                                                              ttAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGt                                                                                                                             >  2:1094449/1‑54 (MQ=255)
                                                                                                aGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTAc                                      <  1:1325171/139‑1 (MQ=255)
                                                                                                     tttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGc                                                                     <  1:800309/103‑1 (MQ=255)
                                                                                                     tttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGc                                                                     >  2:800309/1‑103 (MQ=255)
                                                                                                      ttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                  >  1:1128627/1‑121 (MQ=255)
                                                                                                      ttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                  <  2:1128627/121‑1 (MQ=255)
                                                                                                        ggcggcAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGACAGCgg                                               >  1:1063926/1‑122 (MQ=255)
                                                                                                        ggcggcAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGACAGCgg                                               <  2:1063926/122‑1 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAg                                                                                                       <  1:823565/48‑1 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAg                                                                                                       >  2:1209202/1‑48 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAg                                                                                                       >  2:823565/1‑48 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAg                                                                                                       <  1:1209202/48‑1 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                                   >  2:415704/1‑68 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                                   <  1:415704/68‑1 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                    >  1:936534/1‑83 (MQ=255)
                                                                                                                          ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                    <  2:936534/83‑1 (MQ=255)
                                                                                                                           cGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                               <  2:48232/103‑1 (MQ=255)
                                                                                                                           cGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                               >  1:48232/1‑103 (MQ=255)
                                                                                                                                    aTTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAt  <  2:207565/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAT  >  NC_000913/3598422‑3598692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑