Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,355 C→T P340S (CCA→TCA)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,3550CT100.0% 183.0 / NA 58P340S (CCA→TCA) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

ATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTC  >  NC_000913/323221‑323490
                                                                                                                                      |                                                                                                                                       
aTAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAAt                                                                                                                                     >  1:545891/1‑139 (MQ=255)
                   gCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGATTACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCg                                                                                                                  <  1:634041/139‑1 (MQ=255)
                            tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         >  2:1053152/1‑139 (MQ=255)
                            tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         <  1:1295081/139‑1 (MQ=255)
                            tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         <  2:919576/139‑1 (MQ=255)
                                            ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                            >  2:662298/1‑104 (MQ=255)
                                            ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                            <  1:662298/104‑1 (MQ=255)
                                            ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                         <  2:545891/139‑1 (MQ=255)
                                                  tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                                   <  2:971417/139‑1 (MQ=255)
                                                  tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                                   <  2:1344886/139‑1 (MQ=255)
                                                         gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                          <  1:170076/109‑1 (MQ=255)
                                                         gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                          >  2:170076/1‑109 (MQ=255)
                                                             cGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTACTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                                                        <  1:1053152/139‑1 (MQ=255)
                                                              ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                                 <  2:1110769/81‑1 (MQ=255)
                                                              ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                                 >  1:1110769/1‑81 (MQ=255)
                                                              ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       >  1:934480/1‑139 (MQ=255)
                                                                ggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  2:805856/1‑139 (MQ=255)
                                                                               ttGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                                                <  2:503048/97‑1 (MQ=255)
                                                                               ttGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                                                >  1:503048/1‑97 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                            >  2:416336/1‑68 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                            <  1:416336/68‑1 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                     >  1:386042/1‑139 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                     >  2:798400/1‑139 (MQ=255)
                                                                                  tCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  2:1023631/121‑1 (MQ=255)
                                                                                  tCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  1:1023631/1‑121 (MQ=255)
                                                                                      gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  2:169282/117‑1 (MQ=255)
                                                                                      gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  1:169282/1‑117 (MQ=255)
                                                                                      gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACa                                               >  2:262234/1‑139 (MQ=255)
                                                                                            cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt                                         >  2:528979/1‑139 (MQ=255)
                                                                                            cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt                                         >  1:1384521/1‑139 (MQ=255)
                                                                                               cATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTc                                      <  2:934480/139‑1 (MQ=255)
                                                                                                      gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                           <  2:386466/111‑1 (MQ=255)
                                                                                                      gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                           >  1:386466/1‑111 (MQ=255)
                                                                                                            aTGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         >  2:427713/1‑59 (MQ=255)
                                                                                                            aTGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         <  1:427713/59‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                         <  2:470410/67‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                         >  1:470410/1‑67 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGa                                                 <  1:255977/107‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGa                                                 >  2:255977/1‑107 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTCgg                                                                                                          <  1:22990/50‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTCgg                                                                                                          >  2:22990/1‑50 (MQ=255)
                                                                                                                      ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaaaa               >  1:1367430/1‑139 (MQ=255)
                                                                                                                           tttATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGAt          >  2:1367907/1‑139 (MQ=255)
                                                                                                                              aTCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                           >  2:1198518/1‑87 (MQ=255)
                                                                                                                              aTCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                           <  1:1198518/87‑1 (MQ=255)
                                                                                                                              aTCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAAATGAtttt       >  1:1109633/1‑139 (MQ=255)
                                                                                                                               tCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGTGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       <  2:29383/74‑1 (MQ=255)
                                                                                                                               tCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGTGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       >  1:29383/1‑74 (MQ=255)
                                                                                                                                ccAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                                                <  2:1048896/48‑1 (MQ=255)
                                                                                                                                ccAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                                                >  1:1048896/1‑48 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       >  1:942286/1‑70 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       <  2:942286/70‑1 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                                                       <  2:495819/86‑1 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCg                                    >  2:474392/1‑105 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCg                                    <  1:474392/105‑1 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTc  >  2:618585/1‑139 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTc  >  1:456975/1‑139 (MQ=255)
                                                                                                                                   gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTc  <  1:262234/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
ATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTC  >  NC_000913/323221‑323490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑