Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,694,840 G→A H133Y (CAT→TAT)  bcsA ← cellulose synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,694,8400GA100.0% 144.5 / NA 45H133Y (CAT→TAT) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

TAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694705‑3694971
                                                                                                                                       |                                                                                                                                   
tAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtg                                                                                                                                  <  2:202002/139‑1 (MQ=255)
tAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtg                                                                                                                                  <  2:107721/139‑1 (MQ=255)
   aCTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTgtgt                                                                                                                               >  1:647456/1‑139 (MQ=255)
   aCTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTgtgt                                                                                                                               >  1:882985/1‑139 (MQ=255)
     tGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGttt                                                                                                                             <  2:1039629/139‑1 (MQ=255)
              gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:1223994/139‑1 (MQ=255)
              gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:582804/139‑1 (MQ=255)
                gTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGATGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAcg                                                                                                                  <  1:271303/139‑1 (MQ=255)
                              tAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTc                                                                                                    >  1:883102/1‑139 (MQ=255)
                               aaCGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCg                                                                                                   <  1:551816/139‑1 (MQ=255)
                                accccgATTAACGCCAGTATCACCGAGAAGGTGACGATGATACCTAGGATCTACCGCCCCTCCTCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTCTTATGGCTAACGCGCTCAGGCAGCTCGt                                                                                                  <  1:905887/134‑1 (MQ=255)
                                aCGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGt                                                                                                  <  2:675705/139‑1 (MQ=255)
                                   cAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCa                                                                                               >  1:589158/1‑139 (MQ=255)
                                      aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                              <  2:907475/137‑1 (MQ=255)
                                      aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                              >  1:907475/1‑137 (MQ=255)
                                      aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATcc                                                                                            >  1:870805/1‑139 (MQ=255)
                                           cGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAt                                                                                          <  2:590509/136‑1 (MQ=255)
                                           cGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAt                                                                                          >  1:590509/1‑136 (MQ=255)
                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtgt                                                                                                                                 >  1:833517/1‑92 (MQ=255)
                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtgt                                                                                                                                 <  2:833517/92‑1 (MQ=255)
                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtgt                                                                                                                                 <  1:364336/92‑1 (MQ=255)
                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtgt                                                                                                                                 >  2:364336/1‑92 (MQ=255)
                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  1:920871/1‑105 (MQ=255)
                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:920871/105‑1 (MQ=255)
                                                             ggTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAata                                                                     <  2:647456/139‑1 (MQ=255)
                                                             ggTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAata                                                                     <  2:882985/139‑1 (MQ=255)
                                                                               gATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCa                                                                                                            >  1:946488/1‑82 (MQ=255)
                                                                               gATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCa                                                                                                            <  2:946488/82‑1 (MQ=255)
                                                                                    aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCg                                              >  1:186316/1‑139 (MQ=255)
                                                                                        ccgcGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCt                                                                                                              <  2:1384415/71‑1 (MQ=255)
                                                                                        ccgcGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCt                                                                                                              >  1:1384415/1‑71 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCGTCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  1:814900/1‑44 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  2:116075/1‑44 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:116075/44‑1 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:814900/44‑1 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTg                                                                >  2:1346975/1‑96 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTg                                                                <  1:1346975/96‑1 (MQ=255)
                                                                                                             aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTg                     >  1:181935/1‑139 (MQ=255)
                                                                                                                  gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGa                >  2:630726/1‑139 (MQ=255)
                                                                                                                       tttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAc           <  2:589158/139‑1 (MQ=255)
                                                                                                                        ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  1:89830/139‑1 (MQ=255)
                                                                                                                        ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  2:870805/139‑1 (MQ=255)
                                                                                                                              cATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGtc                                                                                  <  1:917307/61‑2 (MQ=255)
                                                                                                                              cATCCAGATACTGTGTTGTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTa                                                                                  >  2:917307/1‑61 (MQ=255)
                                                                                                                                tCCAGATACTGTGTTTTATGGCTAACGCGATCAGGCAGCTCGTTCATCCATTCATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATa  >  1:849434/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694705‑3694971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: