Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,773,602 T→C V441A (GTT→GCT)  mtlA → mannitol‑specific PTS enzyme: IIA, IIB and IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,773,6020TC100.0% 181.3 / NA 51V441A (GTT→GCT) mtlAmannitol‑specific PTS enzyme: IIA, IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/26);  total (25/26)

CGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGC  >  NC_000913/3773468‑3773723
                                                                                                                                      |                                                                                                                         
cGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg                                                                                                                       <  1:586299/139‑1 (MQ=255)
    cTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCTAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAgg                                                                                                                   >  2:1110924/1‑139 (MQ=255)
        gTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGTCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                               <  2:1395638/139‑1 (MQ=255)
        gTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCTAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                               <  1:1110924/139‑1 (MQ=255)
             aaaaTTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCata                                                                                                          <  2:80831/139‑1 (MQ=255)
                 ttCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg                                                                                                                       >  2:571700/1‑122 (MQ=255)
                 ttCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg                                                                                                                       <  1:571700/122‑1 (MQ=255)
                                     cAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  <  1:56565/139‑1 (MQ=255)
                                        agttCTGTTTCTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCCCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               <  1:868802/137‑1 (MQ=255)
                                            cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCTTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                           <  2:1067706/139‑1 (MQ=255)
                                            cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                           <  1:784400/139‑1 (MQ=255)
                                                  cTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTacac                                                                     >  2:203975/1‑139 (MQ=255)
                                                    aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCa                                                                   >  1:595236/1‑139 (MQ=255)
                                                       aGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGcc                                                                            <  1:360796/127‑1 (MQ=255)
                                                       aGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGcc                                                                            >  2:360796/1‑127 (MQ=255)
                                                           cgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               <  1:157393/120‑1 (MQ=255)
                                                           cgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               >  2:157393/1‑120 (MQ=255)
                                                             aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCt                                                                                       <  2:1428839/110‑1 (MQ=255)
                                                             aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCt                                                                                       >  1:1428839/1‑110 (MQ=255)
                                                              tCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGGAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACAACAGCCTGACCGa                                                         >  1:278620/1‑139 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                               <  2:1367047/75‑1 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                               >  1:1367047/1‑75 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCt                                                                                       >  1:991394/1‑99 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCt                                                                                       <  2:991394/99‑1 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc                                                                                >  1:42103/1‑106 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc                                                                                <  2:42103/106‑1 (MQ=255)
                                                                        gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGtt                                               >  1:1037663/1‑139 (MQ=255)
                                                                                aTGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  >  1:531924/1‑96 (MQ=255)
                                                                                aTGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  <  2:531924/96‑1 (MQ=255)
                                                                                   tgGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgctg                                           <  1:403246/132‑1 (MQ=255)
                                                                                   tgGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgctg                                           >  2:403246/1‑132 (MQ=255)
                                                                                          cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                         >  1:1421389/1‑127 (MQ=255)
                                                                                          cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                         <  2:1421389/127‑1 (MQ=255)
                                                                                                     acCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  <  1:850719/75‑1 (MQ=255)
                                                                                                     acCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  >  2:850719/1‑75 (MQ=255)
                                                                                                          gacctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAg             >  2:426848/1‑139 (MQ=255)
                                                                                                           acctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCacac                              <  2:169077/121‑1 (MQ=255)
                                                                                                           acctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCacac                              >  1:169077/1‑121 (MQ=255)
                                                                                                           acctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGt            <  2:1037663/139‑1 (MQ=255)
                                                                                                            cctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  <  2:365047/68‑1 (MQ=255)
                                                                                                            cctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa                                                                                  >  1:365047/1‑68 (MQ=255)
                                                                                                                 ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGa      >  2:839165/1‑139 (MQ=255)
                                                                                                                   gAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               <  2:1461772/64‑1 (MQ=255)
                                                                                                                   gAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               >  1:1461772/1‑64 (MQ=255)
                                                                                                                     aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               <  2:1201084/62‑1 (MQ=255)
                                                                                                                     aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                               >  1:1201084/1‑62 (MQ=255)
                                                                                                                     aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc  >  1:14347/1‑139 (MQ=255)
                                                                                                                           tATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc                                                                >  2:878955/1‑71 (MQ=255)
                                                                                                                           tATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc                                                                <  1:878955/71‑1 (MQ=255)
                                                                                                                                  cAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGcc                                                                            <  1:1436433/52‑1 (MQ=255)
                                                                                                                                  cAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGcc                                                                            >  2:1436433/1‑52 (MQ=255)
                                                                                                                                      |                                                                                                                         
CGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGC  >  NC_000913/3773468‑3773723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑