Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,304	G→A	*160* (TAG→TAA)	coaD →	pantetheine‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,304	0	G	A	100.0%	157.9 / NA	49	*160* (TAG→TAA)	coaD	pantetheine‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/25);  total (24/25)

TGTGTTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCG  >  NC_000913/3810169‑3810438                                                                                                                                        |                                                                                                                                       tgtgtTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTagcgtaacgt                                                                                                                                     >  2:726302/1‑139 (MQ=255)       tCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGc                                                                                                                               >  2:413561/1‑139 (MQ=255)         tGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                                             <  2:721919/139‑1 (MQ=255)         tGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                                             <  2:1452752/139‑1 (MQ=255)           aTGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                                           <  2:86401/139‑1 (MQ=255)           aTGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                                           <  2:797136/139‑1 (MQ=255)            tGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                                          <  2:178859/139‑1 (MQ=255)                 tCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTAt                                                                                                                     >  1:494641/1‑139 (MQ=255)                           ggTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgc                                                                                                           >  1:1033408/1‑139 (MQ=255)                           ggTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgc                                                                                                           >  2:542546/1‑139 (MQ=255)                            gTCGTTTATCTCTTCATCGTTGGTGAAACAGGTGGCGCGCCATCAGGGCTATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcg                                                                                                          >  1:952353/1‑139 (MQ=255)                               gTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcgc                                                                                                       >  1:610271/1‑139 (MQ=255)                                tttATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                                      <  2:952353/139‑1 (MQ=255)                                    tctcTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGaa                                                                                                  <  1:111796/139‑1 (MQ=255)                                     ctctTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                                 <  2:1033408/139‑1 (MQ=255)                                     ctctTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                                 <  1:726302/139‑1 (MQ=255)                                               ttGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                                 >  2:737950/1‑129 (MQ=255)                                               ttGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                                 <  1:737950/129‑1 (MQ=255)                                                 ggTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCAc                                                                                     >  2:422891/1‑139 (MQ=255)                                                            tGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATa                                                                          >  2:1340357/1‑139 (MQ=255)                                                             ggCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATg                                                                                                                         >  2:449047/1‑91 (MQ=255)                                                             ggCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATg                                                                                                                         <  1:449047/91‑1 (MQ=255)                                                                  gcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATaaaatgt                                                                    <  1:1340357/139‑4 (MQ=255)                                                                   ccATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGtt                                                                   >  1:1079762/1‑139 (MQ=255)                                                                    cATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTg                                                                  >  2:1321521/1‑139 (MQ=255)                                                                      tCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGcc                                                                >  1:268879/1‑139 (MQ=255)                                                                        aGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCg                                                              <  1:1312611/139‑1 (MQ=255)                                                                        aGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCg                                                              <  1:1321521/139‑1 (MQ=255)                                                                              aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgcgc                                                        >  1:916372/1‑139 (MQ=255)                                                                                gTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                                           <  1:1450153/70‑1 (MQ=255)                                                                                gTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                                           >  2:1450153/1‑70 (MQ=255)                                                                                    cccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGttt                                                  >  1:1007781/1‑139 (MQ=255)                                                                                     ccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATg                                                                                                                    >  1:362191/1‑72 (MQ=255)                                                                                     ccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATg                                                                                                                    <  2:362191/72‑1 (MQ=255)                                                                                     ccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTa                                                 <  2:610271/139‑1 (MQ=255)                                                                                               ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                                             <  2:1163204/53‑1 (MQ=255)                                                                                               ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                                             >  1:1163204/1‑53 (MQ=255)                                                                                                cGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAAt                                      <  2:268879/139‑1 (MQ=255)                                                                                                cGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAAt                                      <  2:1007781/139‑1 (MQ=255)                                                                                                             tCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGcac                         <  2:1079762/139‑1 (MQ=255)                                                                                                               aGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgc                                                                                                         >  2:674262/1‑57 (MQ=255)                                                                                                               aGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgc                                                                                                         <  1:674262/57‑1 (MQ=255)                                                                                                                   gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                                      <  2:460754/56‑1 (MQ=255)                                                                                                                   gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                                      >  1:460754/1‑56 (MQ=255)                                                                                                                   gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGa                   >  2:1369516/1‑139 (MQ=255)                                                                                                                       aTGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcg                                                                                                          >  1:1115332/1‑48 (MQ=255)                                                                                                                       aTGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcg                                                                                                          <  2:1115332/48‑1 (MQ=255)                                                                                                                        tGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcg                                                                                                        >  2:55464/1‑49 (MQ=255)                                                                                                                        tGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcg                                                                                                        <  1:55464/49‑1 (MQ=255)                                                                                                                           cGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTc           >  1:1212084/1‑139 (MQ=255)                                                                                                                                 tagcgtaacgTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                                 <  2:312143/47‑1 (MQ=255)                                                                                                                                 tagcgtaacgTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                                 >  1:312143/1‑47 (MQ=255)                                                                                                                                    cgtaacgtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCACGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCg  >  1:1057193/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       TGTGTTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCG  >  NC_000913/3810169‑3810438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑