| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,842,455 | C→T | *445* (TAG→TAA) | adeQ ← | adenine permease, high affinity, adenine:H+ symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,842,455 | 0 | C | T | 98.7% | 239.4 / NA | 75 | *445* (TAG→TAA) | adeQ | adenine permease, high affinity, adenine:H+ symporter |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (40/34); total (41/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGT > NC_000913/3842320‑3842586 | aTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa < 2:1457903/139‑1 (MQ=255) ttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca < 1:1389106/139‑1 (MQ=255) ttCTGGCCACGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca < 1:452710/139‑1 (MQ=255) cGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt < 1:580094/139‑1 (MQ=255) ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCtt < 1:1238362/139‑1 (MQ=255) gATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTc < 1:81118/139‑1 (MQ=255) cAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGc > 1:687863/1‑139 (MQ=255) cccGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACa > 1:1075786/1‑139 (MQ=255) aaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt < 2:1075786/139‑1 (MQ=255) cacTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACcaccaccac > 2:222404/1‑139 (MQ=255) acTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca < 2:192596/139‑1 (MQ=255) tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca < 1:822565/139‑1 (MQ=255) tACCGCCAGCGTGGCGAGCAGTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATTATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca < 1:1227390/139‑1 (MQ=255) aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca > 1:1412519/1‑131 (MQ=255) aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca < 2:1412519/131‑1 (MQ=255) aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac > 1:1354006/1‑139 (MQ=255) cGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa > 2:396793/1‑80 (MQ=255) cGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa < 1:396793/80‑1 (MQ=255) cGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAAGCAGAACTGATGATATCTGGAAACACTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCAACCACCACAACACACAGGTTCAg > 2:441233/1‑139 (MQ=255) ggCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATc > 1:1371511/1‑139 (MQ=255) gCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac < 2:452690/81‑1 (MQ=255) gCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac > 1:452690/1‑81 (MQ=255) cATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCa < 1:490412/139‑1 (MQ=255) tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc < 1:222404/139‑1 (MQ=255) aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata < 2:467153/73‑1 (MQ=255) aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata > 1:467153/1‑73 (MQ=255) aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGa < 1:808407/70‑1 (MQ=255) aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGa > 2:808407/1‑70 (MQ=255) tGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATa > 2:728408/1‑139 (MQ=255) gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac < 2:1365145/94‑1 (MQ=255) gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac > 1:1365145/1‑94 (MQ=255) gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc > 1:1027553/1‑139 (MQ=255) gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc > 1:124845/1‑139 (MQ=255) gTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata < 2:386714/66‑1 (MQ=255) gTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata > 1:386714/1‑66 (MQ=255) ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatg > 1:1397252/1‑139 (MQ=255) cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCg > 2:473554/1‑123 (MQ=255) cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCg < 1:473554/123‑1 (MQ=255) cGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac < 1:792982/50‑1 (MQ=255) cGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac > 2:792982/1‑50 (MQ=255) tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa > 1:116902/1‑131 (MQ=255) tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa < 2:116902/131‑1 (MQ=255) tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAg > 1:1208510/1‑139 (MQ=255) tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc > 1:623234/1‑113 (MQ=255) tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc < 2:623234/113‑1 (MQ=255) tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTAc > 2:986320/1‑139 (MQ=255) aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCg < 1:1086382/71‑1 (MQ=255) aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCg > 2:1086382/1‑71 (MQ=255) aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca > 1:1259408/1‑85 (MQ=255) aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca < 2:1259408/85‑1 (MQ=255) aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCGCACACAGGTTCAGATCGCGCCAgcgc < 1:536293/107‑1 (MQ=255) aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc > 2:536293/1‑107 (MQ=255) cATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATaaa < 2:1354006/139‑1 (MQ=255) aTGATATCTGGAAACCCTCGCCAGTTATTAATccacca < 2:1325564/38‑1 (MQ=255) aTGATATCTGGAAACCCTCGCCAGTTATTAATccacca > 1:1325564/1‑38 (MQ=255) aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCGAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAg > 1:882018/1‑139 (MQ=255) aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAg > 2:1380099/1‑139 (MQ=255) tGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGc > 2:1219429/1‑139 (MQ=255) atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAg > 1:62684/1‑139 (MQ=255) tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc < 2:173326/139‑1 (MQ=255) ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa > 1:928784/1‑139 (MQ=255) gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa > 1:190084/1‑139 (MQ=255) aaCCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATc > 2:564200/1‑139 (MQ=255) cccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca < 2:386370/67‑1 (MQ=255) cccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca > 1:386370/1‑67 (MQ=255) ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCa < 2:441713/84‑1 (MQ=255) ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCa > 1:441713/1‑84 (MQ=255) tCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTc > 2:571572/1‑139 (MQ=255) cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacc > 1:752247/1‑55 (MQ=255) cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacc < 2:752247/55‑1 (MQ=255) cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacc < 1:895940/52‑1 (MQ=255) cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacc > 2:895940/1‑52 (MQ=255) cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc < 2:1249290/129‑1 (MQ=255) cGCCAATTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc > 1:1249290/1‑129 (MQ=255) ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGt > 1:590531/1‑139 (MQ=255) | ATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGT > NC_000913/3842320‑3842586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |