Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,923,070 G→A A204V (GCA→GTA)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,0700GA100.0% 84.5 / NA 27A204V (GCA→GTA) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

GTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAG  >  NC_000913/3922937‑3923191
                                                                                                                                     |                                                                                                                         
gTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTa                                                                                                                      <  2:772070/139‑1 (MQ=255)
            aTGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTANNNCAGTANAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGa                                                                                                          <  2:43563/139‑1 (MQ=255)
              gCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAACTTTATTTACTTTAATCACCACCAGATg                                                                                                        >  1:579432/1‑139 (MQ=255)
                                 gTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCt                                                                                     <  1:52617/139‑1 (MQ=255)
                                   tAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                   >  1:355749/1‑139 (MQ=255)
                                               gTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGttt                                                                       >  2:972845/1‑139 (MQ=255)
                                                ttATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                              >  1:1186320/1‑131 (MQ=255)
                                                ttATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                              <  2:1186320/131‑1 (MQ=255)
                                                         ttataaaCCTTGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                             <  1:708912/135‑1 (MQ=255)
                                                            aaaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          <  1:386767/139‑1 (MQ=255)
                                                             aaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGa                                                         <  1:1301977/139‑1 (MQ=255)
                                                                   ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGat                                                   >  1:89080/1‑139 (MQ=255)
                                                                   ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGat                                                   >  2:1395274/1‑139 (MQ=255)
                                                                                   cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAtt                                                            >  1:243396/1‑114 (MQ=255)
                                                                                   cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAtt                                                            <  2:243396/114‑1 (MQ=255)
                                                                                            cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          <  2:816879/107‑1 (MQ=255)
                                                                                            cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                        <  1:469881/109‑1 (MQ=255)
                                                                                            cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                        >  2:469881/1‑109 (MQ=255)
                                                                                            cAATTTTTTATGATTTTTCTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          >  1:816879/1‑107 (MQ=255)
                                                                                                                    aaaaTTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  <  1:793794/139‑1 (MQ=255)
                                                                                                                    aaaaTTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  <  2:89080/139‑1 (MQ=255)
                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCgg                                          >  2:675352/1‑87 (MQ=255)
                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCgg                                          <  1:675352/87‑1 (MQ=255)
                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  >  2:546502/1‑127 (MQ=255)
                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  <  1:546502/127‑1 (MQ=255)
                                                                                                                                 aTTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          >  2:1187317/1‑70 (MQ=255)
                                                                                                                                 aTTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          <  1:1187317/70‑1 (MQ=255)
                                                                                                                                     |                                                                                                                         
GTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAG  >  NC_000913/3922937‑3923191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: