Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,938,615 C→T S130L (TCG→TTG)  rbsR → transcriptional repressor of ribose metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,938,6150CT100.0% 271.0 / NA 83S130L (TCG→TTG) rbsRtranscriptional repressor of ribose metabolism
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/44);  total (39/44)

CTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATA  >  NC_000913/3938481‑3938749
                                                                                                                                      |                                                                                                                                      
ctTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGt                                                                                                                                    >  2:377251/1‑139 (MQ=255)
     aaCGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAAt                                                                                                                               >  2:699839/1‑139 (MQ=255)
          ggTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGc                                                                                                                          >  1:247553/1‑139 (MQ=255)
            ttATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCaa                                                                                                                        <  2:247553/139‑1 (MQ=255)
                      gTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGa                                                                                                              >  1:1435198/1‑139 (MQ=255)
                      gTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGa                                                                                                              >  1:824877/1‑139 (MQ=255)
                            tGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGc                                                                                                        <  2:651183/139‑1 (MQ=255)
                              cAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGa                                                                                                              <  2:591157/131‑1 (MQ=255)
                              cAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGa                                                                                                              >  1:591157/1‑131 (MQ=255)
                               aaTACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTa                                                                                                     <  1:598673/139‑1 (MQ=255)
                                aTACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTAc                                                                                                    <  1:939658/139‑1 (MQ=255)
                                    cGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGaaa                                                                                                                                <  2:523/107‑1 (MQ=255)
                                    cGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGaaa                                                                                                                                >  1:523/1‑107 (MQ=255)
                                    cGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             <  2:661732/126‑1 (MQ=255)
                                    cGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             >  1:661732/1‑126 (MQ=255)
                                    cGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACtgtg                                                                                                >  1:1420681/1‑139 (MQ=255)
                                      aaGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGtgat                                                                                              >  2:1046411/1‑139 (MQ=255)
                                        ggCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCaa                                                                                                                        >  1:1363961/1‑111 (MQ=255)
                                        ggCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCaa                                                                                                                        <  2:1363961/111‑1 (MQ=255)
                                         gCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTgatgat                                                                                           <  2:598367/139‑1 (MQ=255)
                                            aTGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGa                                                                                        <  1:795728/139‑1 (MQ=255)
                                             tGAACAGCGGATGAATCGCAGTCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTa                                                                                                     <  2:942905/125‑1 (MQ=255)
                                             tGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTa                                                                                                     >  1:942905/1‑125 (MQ=255)
                                              gAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACt                                                                                      <  2:22161/139‑1 (MQ=255)
                                                 cAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTggg                                                                                   <  1:758611/139‑1 (MQ=255)
                                                  aGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTg                                                                                                                                   >  2:239737/1‑90 (MQ=255)
                                                  aGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTg                                                                                                                                   <  1:239737/90‑1 (MQ=255)
                                                   gCGGATGAATCGCAATCTAGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACt                                                                                      >  1:1465875/1‑134 (MQ=255)
                                                   gCGGATGAATCGCAATCTAGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACt                                                                                      <  2:1465875/134‑1 (MQ=255)
                                                    cGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTc                                                                                >  2:126127/1‑139 (MQ=255)
                                                     ggATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTcc                                                                               >  1:1293210/1‑139 (MQ=255)
                                                         gAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATc                                                                                                                 <  1:1428014/101‑1 (MQ=255)
                                                         gAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATc                                                                                                                 >  2:1428014/1‑101 (MQ=255)
                                                            tCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGAt                                                                        >  2:1453653/1‑139 (MQ=255)
                                                                aaTCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATc                                                                                                                 >  1:136484/1‑94 (MQ=255)
                                                                aaTCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATc                                                                                                                 <  2:136484/94‑1 (MQ=255)
                                                                 aTCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGa                                                                   <  1:464020/139‑1 (MQ=255)
                                                                    tGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAg                                                                <  1:126127/139‑1 (MQ=255)
                                                                    tGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAg                                                                <  1:1211439/139‑1 (MQ=255)
                                                                         aCGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTgatgat                                                                                           >  2:403565/1‑107 (MQ=255)
                                                                         aCGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTgatgat                                                                                           <  1:403565/107‑1 (MQ=255)
                                                                            cTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACa                                                                                                            <  2:410866/87‑1 (MQ=255)
                                                                            cTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACa                                                                                                            >  1:410866/1‑87 (MQ=255)
                                                                              gATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             <  2:1088341/84‑1 (MQ=255)
                                                                              gATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             <  2:759347/84‑1 (MQ=255)
                                                                              gATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             >  1:759347/1‑84 (MQ=255)
                                                                              gATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             >  1:1088341/1‑84 (MQ=255)
                                                                              gATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTAtt                                                      >  2:783621/1‑139 (MQ=255)
                                                                                   aaaaaCGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGa                                                 <  1:653361/139‑1 (MQ=255)
                                                                                        cgcgTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACt                                            <  2:824877/139‑1 (MQ=255)
                                                                                                    ttGCTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCg                                <  2:269934/139‑1 (MQ=255)
                                                                                                       cTGTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGgag                             >  1:312707/1‑139 (MQ=255)
                                                                                                         gTTACTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGAc                           >  2:601854/1‑139 (MQ=255)
                                                                                                             cTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             >  1:1247003/1‑53 (MQ=255)
                                                                                                             cTGTGCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGAc                                                                                                             <  2:1247003/53‑1 (MQ=255)
                                                                                                              tgtgCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGc                      <  2:748795/139‑1 (MQ=255)
                                                                                                              tgtgCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGc                      >  1:47463/1‑139 (MQ=255)
                                                                                                              tgtgCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGc                      <  2:155478/139‑1 (MQ=255)
                                                                                                                tgCACCGAAACGCATCAACCTTTGCGTGAAATCATg                                                                                                                           >  2:1024348/1‑36 (MQ=255)
                                                                                                                tgCACCGAAACGCATCAACCTTTGCGTGAAATCATg                                                                                                                           <  1:1024348/36‑1 (MQ=255)
                                                                                                                tgCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCaa                    <  1:1046411/139‑1 (MQ=255)
                                                                                                                tgCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCaa                    <  2:1420681/139‑1 (MQ=255)
                                                                                                                 gCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGATTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGga                              <  2:1390725/128‑1 (MQ=255)
                                                                                                                 gCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCgg                               >  2:184840/1‑127 (MQ=255)
                                                                                                                 gCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCgg                               <  1:184840/127‑1 (MQ=255)
                                                                                                                 gCACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGga                              >  1:1390725/1‑128 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGa                                                                                        >  2:902695/1‑69 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGa                                                                                        <  1:902695/69‑1 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAg                                                                <  1:171884/93‑1 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAg                                                                <  1:1441255/93‑1 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAg                                                                >  2:1441255/1‑93 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAg                                                                >  2:171884/1‑93 (MQ=255)
                                                                                                                  cACCGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACg                  <  2:1293210/139‑1 (MQ=255)
                                                                                                                    ccGAAACGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCa                >  1:236711/1‑139 (MQ=255)
                                                                                                                       aaaCGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGt                                                                                                          >  2:168486/1‑46 (MQ=255)
                                                                                                                       aaaCGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGt                                                                                                          <  1:168486/46‑1 (MQ=255)
                                                                                                                       aaaCGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAata             >  2:243167/1‑139 (MQ=255)
                                                                                                                        aaCGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAatat            <  1:243167/139‑1 (MQ=255)
                                                                                                                          cGCATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCt          >  2:1007496/1‑139 (MQ=255)
                                                                                                                            cATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCaa                    <  1:670630/127‑1 (MQ=255)
                                                                                                                            cATCAACCTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCaa                    >  2:670630/1‑127 (MQ=255)
                                                                                                                                  ccTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATa  <  1:601854/139‑1 (MQ=255)
                                                                                                                                  ccTTTGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATa  <  1:1453653/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATA  >  NC_000913/3938481‑3938749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑