Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,123	G→A	R34C (CGC→TGC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,123	0	G	A	100.0%	300.9 / NA	92	R34C (CGC→TGC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/50);  total (42/50)

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAG  >  NC_000913/4116987‑4117255                                                                                                                                          |                                                                                                                                     ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACt                                                                                                                                    >  1:556467/1‑139 (MQ=255)  cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTg                                                                                                                                   <  1:949910/139‑1 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                                 >  2:1160248/1‑139 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                                 >  1:358756/1‑139 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGAGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                <  1:1443612/139‑1 (MQ=255)      aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               >  1:189453/1‑139 (MQ=255)       gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTCGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                              <  2:214462/139‑1 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                            >  2:311987/1‑139 (MQ=255)          gCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGc                                                                                                                           <  2:176949/139‑1 (MQ=255)          gCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGc                                                                                                                           <  2:556467/139‑1 (MQ=255)            aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         <  2:189453/139‑1 (MQ=255)            aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         <  2:1102520/139‑1 (MQ=255)              tttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAAt                                                                                                                       <  1:311987/139‑1 (MQ=255)                   tCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         >  1:469689/1‑132 (MQ=255)                   tCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         <  2:469689/132‑1 (MQ=255)                      gAACTGATATCGGCTTTCGCCAGCACTTCTACCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTg                                                                                                                <  2:358756/139‑1 (MQ=255)                      gAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCGGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTgg                                                                                                               <  2:993986/139‑1 (MQ=255)                            atatCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                >  1:176700/1‑116 (MQ=255)                            atatCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                <  2:176700/116‑1 (MQ=255)                            atatCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         <  2:582244/123‑1 (MQ=255)                            atatCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         >  1:582244/1‑123 (MQ=255)                             tatCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                <  1:17483/115‑1 (MQ=255)                             tatCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                >  2:17483/1‑115 (MQ=255)                              atCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  2:460492/139‑1 (MQ=255)                              atCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  2:118088/139‑1 (MQ=255)                              atCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  1:336515/139‑1 (MQ=255)                                 ggCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAAt                                                                                                                       >  2:1176925/1‑120 (MQ=255)                                 ggCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAAt                                                                                                                       <  1:1176925/120‑1 (MQ=255)                                      tCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                                                                               >  1:1281287/1‑139 (MQ=255)                                        gCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGACTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         <  2:915195/111‑1 (MQ=255)                                        gCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGACTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                         >  1:915195/1‑111 (MQ=255)                                             cACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                        <  2:120902/139‑1 (MQ=255)                                                        aGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATc                                                                                                           <  1:621643/110‑1 (MQ=255)                                                        aGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATc                                                                                                           >  2:621643/1‑110 (MQ=255)                                                          cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                            >  1:462674/1‑139 (MQ=255)                                                           gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                              >  2:928121/1‑88 (MQ=255)                                                           gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                              <  1:928121/88‑1 (MQ=255)                                                             ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       >  2:1220132/1‑109 (MQ=255)                                                             ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  1:1220132/109‑1 (MQ=255)                                                                gCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                                      <  1:1160248/139‑1 (MQ=255)                                                                           cccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                      <  2:388162/112‑1 (MQ=255)                                                                           cccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                      >  1:388162/1‑112 (MQ=255)                                                                                aTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGa                                                      <  2:1281287/139‑1 (MQ=255)                                                                                  ttCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGata                                                    <  2:462674/139‑1 (MQ=255)                                                                                     cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGatatat                                                 >  2:466949/1‑139 (MQ=255)                                                                                         gggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                >  1:1144539/1‑56 (MQ=255)                                                                                         gggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                                <  2:1144539/56‑1 (MQ=255)                                                                                          ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               >  1:827051/1‑56 (MQ=255)                                                                                          ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               <  2:827051/56‑1 (MQ=255)                                                                                          ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                            >  2:800841/1‑139 (MQ=255)                                                                                            tCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               <  1:855202/54‑1 (MQ=255)                                                                                            tCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               >  2:855202/1‑54 (MQ=255)                                                                                            tCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                          >  1:544409/1‑139 (MQ=255)                                                                                               tgtTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               >  1:886100/1‑51 (MQ=255)                                                                                               tgtTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                               <  2:886100/51‑1 (MQ=255)                                                                                               tgtTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                       <  2:544409/139‑1 (MQ=255)                                                                                                gtTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                                      <  1:1152725/139‑1 (MQ=255)                                                                                                 ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                     <  1:872361/139‑1 (MQ=255)                                                                                                    tACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  1:847754/70‑1 (MQ=255)                                                                                                    tACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       >  2:847754/1‑70 (MQ=255)                                                                                                      cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGtt                                >  1:254882/1‑139 (MQ=255)                                                                                                         aaCCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                             >  2:1121225/1‑139 (MQ=255)                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtgg                                                                              >  1:1089118/1‑88 (MQ=255)                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtgg                                                                              <  2:1089118/88‑1 (MQ=255)                                                                                                              ggTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       >  1:320343/1‑60 (MQ=255)                                                                                                              ggTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       >  2:729156/1‑60 (MQ=255)                                                                                                              ggTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  2:320343/60‑1 (MQ=255)                                                                                                              ggTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  1:729156/60‑1 (MQ=255)                                                                                                               gTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTcc                       >  1:479787/1‑139 (MQ=255)                                                                                                                ttttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                 >  1:717009/1‑80 (MQ=255)                                                                                                                ttttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                 <  2:717009/80‑1 (MQ=255)                                                                                                                  ttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGTTGGTGCCGTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                    <  1:103966/139‑1 (MQ=255)                                                                                                                  ttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                    <  2:1318962/139‑1 (MQ=255)                                                                                                                  ttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                    <  1:800841/139‑1 (MQ=255)                                                                                                                  ttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                    <  1:1121225/139‑1 (MQ=255)                                                                                                                   tGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTa                   >  2:850089/1‑139 (MQ=255)                                                                                                                     ggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGa                                                      <  2:1467566/102‑1 (MQ=255)                                                                                                                     ggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGa                                                      >  1:1467566/1‑102 (MQ=255)                                                                                                                     ggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGt                 >  1:1032978/1‑139 (MQ=255)                                                                                                                      gTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  2:1248023/52‑1 (MQ=255)                                                                                                                      gTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       >  1:1248023/1‑52 (MQ=255)                                                                                                                         gATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                       >  1:663601/1‑113 (MQ=255)                                                                                                                         gATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCata             >  2:393255/1‑139 (MQ=255)                                                                                                                         gATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCAGTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                       <  2:663601/113‑1 (MQ=255)                                                                                                                                 cAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg     <  2:254882/139‑1 (MQ=255)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       <  2:1187032/38‑1 (MQ=255)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                       >  1:1187032/1‑38 (MQ=38)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                 >  1:462111/1‑60 (MQ=255)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                 <  2:462111/60‑1 (MQ=255)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                           <  2:945802/66‑1 (MQ=255)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                           >  1:945802/1‑66 (MQ=255)                                                                                                                                    aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAg  >  1:1197212/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                     CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAG  >  NC_000913/4116987‑4117255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑