Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,678	G→A	L222L (CTG→CTA)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,678	0	G	A	100.0%	148.6 / NA	46	L222L (CTG→CTA)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/22);  total (24/22)

TGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCG  >  NC_000913/4156545‑4156808                                                                                                                                      |                                                                                                                                   tGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGc                                                                                                                               <  1:1167599/139‑1 (MQ=255)       ccGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCg                                                                                                                         <  2:901145/139‑1 (MQ=255)                                         aCGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTgg                                                                                       >  1:18843/1‑139 (MQ=255)                                          cGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGc                                                                                      >  2:1040196/1‑139 (MQ=255)                                          cGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGc                                                                                      >  1:1363737/1‑139 (MQ=255)                                               tGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGc                                                                                                                               <  2:507947/93‑1 (MQ=255)                                               tGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGc                                                                                                                               >  1:507947/1‑93 (MQ=255)                                                gCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATg                                                                                <  2:1015263/139‑1 (MQ=255)                                                       tGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCg                                                                                     >  1:1032819/1‑127 (MQ=255)                                                       tGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCg                                                                                     <  2:1032819/127‑1 (MQ=255)                                                         aCCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGagcagc                                                                       >  2:445269/1‑139 (MQ=255)                                                                 gAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               <  2:608523/139‑1 (MQ=255)                                                                      cAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGtt                                                          <  1:350015/139‑1 (MQ=255)                                                                         aacaacTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       <  1:445269/139‑1 (MQ=255)                                                                              cTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            <  2:633356/81‑1 (MQ=255)                                                                              cTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            >  1:633356/1‑81 (MQ=255)                                                                                   tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               <  2:767340/121‑1 (MQ=255)                                                                                   tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               >  1:767340/1‑121 (MQ=255)                                                                                      gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                          >  1:1392298/1‑59 (MQ=255)                                                                                      gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                          <  2:1392298/59‑1 (MQ=255)                                                                                      gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               <  1:11700/118‑1 (MQ=255)                                                                                      gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               >  2:11700/1‑118 (MQ=255)                                                                                          ggCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACg                                                     <  1:801608/124‑1 (MQ=255)                                                                                          ggCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACg                                                     >  2:801608/1‑124 (MQ=255)                                                                                                    cTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCg                                                                                                                         <  2:1300978/46‑1 (MQ=255)                                                                                                    cTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCg                                                                                                                         >  1:1300978/1‑46 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            >  1:265882/1‑54 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            <  2:265882/54‑1 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       >  1:76788/1‑107 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       <  2:76788/107‑1 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       <  2:990132/139‑1 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       >  1:990132/1‑139 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       >  1:720754/1‑139 (MQ=255)                                                                                                         ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       >  1:1303841/1‑139 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCt                                                                                                                   <  2:1123317/45‑1 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCt                                                                                                                   >  1:1123317/1‑45 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGc                                                                <  2:1187580/96‑1 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGc                                                                >  1:1187580/1‑96 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                                                   <  2:187798/109‑1 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                                                   >  1:187798/1‑109 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTaa                     >  2:1041492/1‑139 (MQ=255)                                                                                                           cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTCAACGGTATGCCGATGGGTACGCGGATTTTAGCTTaa                     >  2:923611/1‑139 (MQ=255)                                                                                                                           aaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAg     >  1:874772/1‑139 (MQ=255)                                                                                                                             cgcgGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       >  2:330079/1‑87 (MQ=255)                                                                                                                             cgcgGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       <  1:330079/87‑1 (MQ=255)                                                                                                                              gcggcgCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGgcg  <  1:1041492/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   TGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCG  >  NC_000913/4156545‑4156808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑