Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,158,538 C→T H17Y (CAT→TAT)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,5380CT100.0% 324.5 / NA 99H17Y (CAT→TAT) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (54/45);  total (54/45)

AGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACC  >  NC_000913/4158409‑4158663
                                                                                                                                 |                                                                                                                             
agtcagAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGc                                                                                                                      >  2:348878/1‑139 (MQ=255)
     gAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCg                                                                                                                 >  2:1399967/1‑139 (MQ=255)
      aaTGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCgg                                                                                                                >  2:1189175/1‑139 (MQ=255)
            tGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGAtt                                                                                                          >  1:49087/1‑139 (MQ=255)
                   gATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTAtttt                                                                                                                          >  1:1279799/1‑116 (MQ=255)
                   gATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTAtttt                                                                                                                          <  2:1279799/116‑1 (MQ=255)
                    aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCAc                                                                                                  >  2:1231894/1‑139 (MQ=255)
                         cGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                                                                                             <  1:1150582/139‑1 (MQ=255)
                         cGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                                                                                             <  2:956296/139‑1 (MQ=255)
                             cATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagccag                                                                                         >  2:556376/1‑139 (MQ=255)
                                      tCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCtt                                                                                >  1:715288/1‑139 (MQ=255)
                                        tACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAg                                                                              <  1:348878/139‑1 (MQ=255)
                                          ccTATCGCCATGAACTATGGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTg                                                                                                                   <  1:866844/100‑1 (MQ=255)
                                          ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTg                                                                                                                   >  2:866844/1‑100 (MQ=255)
                                          ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCg                                                                            >  1:1434299/1‑139 (MQ=255)
                                          ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCg                                                                            >  1:479913/1‑139 (MQ=255)
                                           cTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagccagc                                                                                        <  2:465712/126‑1 (MQ=255)
                                           cTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagccagc                                                                                        >  1:465712/1‑126 (MQ=255)
                                            tATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCggg                                                                          >  2:268774/1‑139 (MQ=255)
                                            tATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCggg                                                                          <  1:745131/139‑1 (MQ=255)
                                             aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGc                                                                         >  1:1203194/1‑139 (MQ=255)
                                              tCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCa                                                                        <  2:595318/139‑1 (MQ=255)
                                               cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                                                                                             <  2:85980/117‑1 (MQ=255)
                                               cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                                                                                             >  1:85980/1‑117 (MQ=255)
                                               cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCaa                                                                       >  2:219494/1‑139 (MQ=255)
                                               cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCa                                                                        >  2:1012051/1‑138 (MQ=255)
                                               cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCa                                                                        <  1:1012051/138‑1 (MQ=255)
                                                 ccATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAAt                                                                     >  2:386130/1‑139 (MQ=255)
                                                  cATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAAtt                                                                    <  2:479913/139‑1 (MQ=255)
                                                    tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCg                                                                  <  2:132345/139‑1 (MQ=255)
                                                          aTCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            >  2:698213/1‑139 (MQ=255)
                                                            cGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTgg                                                          <  2:1203194/139‑1 (MQ=255)
                                                                gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTg                                                                                                      >  2:829084/1‑91 (MQ=255)
                                                                gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTg                                                                                                      <  1:829084/91‑1 (MQ=255)
                                                                     ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTc                                                                                                         <  1:1238126/83‑1 (MQ=255)
                                                                     ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTc                                                                                                         >  2:1238126/1‑83 (MQ=255)
                                                                     ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                 <  2:706207/139‑1 (MQ=255)
                                                                      gaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCt                                                <  1:219494/139‑1 (MQ=255)
                                                                         gatggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTggg                                             <  1:1399967/139‑1 (MQ=255)
                                                                           tggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGt                                                                 >  2:156714/1‑117 (MQ=255)
                                                                           tggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGt                                                                 <  1:156714/117‑1 (MQ=255)
                                                                            ggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGt                                          <  2:49087/139‑1 (MQ=255)
                                                                                  tGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCg                                                                                                                     >  2:1118653/1‑58 (MQ=255)
                                                                                  tGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCg                                                                                                                     <  1:1118653/58‑1 (MQ=255)
                                                                                    aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTg                                                                                                      >  1:496936/1‑71 (MQ=255)
                                                                                    aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTg                                                                                                      <  2:496936/71‑1 (MQ=255)
                                                                                       atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTgg                               >  1:684128/1‑139 (MQ=255)
                                                                                        ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGa                              >  1:240628/1‑139 (MQ=255)
                                                                                        ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGa                              >  1:586616/1‑139 (MQ=255)
                                                                                         tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                             <  2:715288/139‑1 (MQ=255)
                                                                                         tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                             <  2:1434299/139‑1 (MQ=255)
                                                                                           gTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCg                           <  2:586616/139‑1 (MQ=255)
                                                                                            tGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCa                                                                        >  1:1465470/1‑93 (MQ=255)
                                                                                            tGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCa                                                                        <  2:1465470/93‑1 (MQ=255)
                                                                                              aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGc                                                                         <  2:790886/90‑1 (MQ=255)
                                                                                              aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGc                                                                         >  1:790886/1‑90 (MQ=255)
                                                                                                cTTGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCa                      >  1:405281/1‑139 (MQ=255)
                                                                                                 ttGAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                     >  2:469491/1‑139 (MQ=255)
                                                                                                   gAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCg                                                                                                                     <  2:653947/41‑1 (MQ=255)
                                                                                                   gAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCg                                                                                                                     >  1:653947/1‑41 (MQ=255)
                                                                                                   gAGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGcc                   >  2:30996/1‑139 (MQ=255)
                                                                                                    aGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                                                                                             <  2:503885/64‑1 (MQ=255)
                                                                                                    aGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                                                                                             >  1:503885/1‑64 (MQ=255)
                                                                                                    aGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            <  1:770333/97‑1 (MQ=255)
                                                                                                    aGTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            >  2:770333/1‑97 (MQ=255)
                                                                                                     gTACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGt                 >  1:1201867/1‑139 (MQ=255)
                                                                                                      tACCTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                <  2:684128/139‑1 (MQ=255)
                                                                                                         cTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCg                                                                  <  1:801324/86‑1 (MQ=255)
                                                                                                         cTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCg                                                                  >  2:801324/1‑86 (MQ=255)
                                                                                                         cTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTaa                                                               >  1:1102170/1‑89 (MQ=255)
                                                                                                         cTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTaa                                                               <  2:1102170/89‑1 (MQ=255)
                                                                                                         cTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            >  2:9672/1‑92 (MQ=255)
                                                                                                         cTGGTGGCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            <  1:9672/92‑1 (MQ=255)
                                                                                                          tggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt            <  2:240628/139‑1 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagc                                                                                            >  2:98570/1‑58 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagc                                                                                            <  1:98570/58‑1 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            >  1:575983/1‑90 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCt                                                            <  2:575983/90‑1 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGTAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg           >  2:875827/1‑139 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg           >  2:1107593/1‑139 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg           >  1:702968/1‑139 (MQ=255)
                                                                                                           ggtggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg           >  2:629550/1‑139 (MQ=255)
                                                                                                              ggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGAtt                                                                                                          <  1:374907/41‑1 (MQ=255)
                                                                                                              ggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGAtt                                                                                                          >  2:374907/1‑41 (MQ=255)
                                                                                                              ggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTCAAGtgttg        >  1:893375/1‑139 (MQ=255)
                                                                                                              ggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttg        >  2:1035388/1‑139 (MQ=255)
                                                                                                              ggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttg        >  2:904860/1‑139 (MQ=255)
                                                                                                              ggCATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttg        >  2:370474/1‑139 (MQ=255)
                                                                                                                cATTGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTgttgtt      <  1:556376/139‑1 (MQ=255)
                                                                                                                   tGGCTGAACACCGCTATTTTCGGCGTGCGGCAGAtt                                                                                                          >  1:248967/1‑36 (MQ=255)
                                                                                                                   tGGCTGAACACCGCTATTTTCGGCGTGCGGCAGAtt                                                                                                          <  2:248967/36‑1 (MQ=255)
                                                                                                                   tGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCa                                                                                                   <  1:1430308/43‑1 (MQ=255)
                                                                                                                   tGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCa                                                                                                   >  2:1430308/1‑43 (MQ=255)
                                                                                                                   tGGCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc   <  1:386130/139‑1 (MQ=255)
                                                                                                                    ggCTGAACACCGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAcc  <  1:875827/139‑1 (MQ=255)
                                                                                                                              cGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagc                                                                                            >  2:1332287/1‑39 (MQ=255)
                                                                                                                              cGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagc                                                                                            <  1:1332287/39‑1 (MQ=255)
                                                                                                                              cGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                        >  2:1200079/1‑107 (MQ=255)
                                                                                                                              cGCTATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                        <  1:1200079/107‑1 (MQ=255)
                                                                                                                                 |                                                                                                                             
AGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACC  >  NC_000913/4158409‑4158663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑