Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,872	C→T	A833A (GCG→GCA)	mscM ←	mechanosensitive channel protein, miniconductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,872	0	C	T	100.0%	105.0 / NA	33	A833A (GCG→GCA)	mscM	mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

GTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGAT  >  NC_000913/4386758‑4387006                                                                                                                   |                                                                                                                                       gTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGa                                                                                                                <  1:369981/139‑1 (MQ=255)       cGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                          <  2:1075085/139‑1 (MQ=255)       cGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                          <  1:398445/139‑1 (MQ=255)        gTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTa                                                                                                         <  1:405325/139‑1 (MQ=255)         tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc                                                                                                        >  2:1419354/1‑139 (MQ=255)         tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc                                                                                                        >  1:385722/1‑139 (MQ=255)          aCCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCATCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                       <  1:1052108/139‑1 (MQ=255)            ccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                     <  1:1419354/139‑1 (MQ=255)            ccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                     <  2:635203/139‑1 (MQ=255)                            gTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                       <  2:1356904/121‑1 (MQ=255)                            gTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                       >  1:1356904/1‑121 (MQ=255)                                tgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCt                                                                                                                     >  2:1331729/1‑103 (MQ=255)                                tgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCt                                                                                                                     <  1:1331729/103‑1 (MQ=255)                                           cAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaa                                                                      >  1:237622/1‑139 (MQ=255)                                                    cagcGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCg                                                             <  1:1201022/139‑1 (MQ=255)                                                       cgcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                     <  2:1124540/96‑1 (MQ=255)                                                       cgcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                     >  1:1124540/1‑96 (MQ=255)                                                         cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                          <  1:199433/89‑1 (MQ=255)                                                         cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                          >  2:199433/1‑89 (MQ=255)                                                                 ttGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAAt                                                                                                                          <  1:213911/65‑1 (MQ=255)                                                                 ttGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAAt                                                                                                                          >  2:213911/1‑65 (MQ=255)                                                                   gcgcACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCg                                              <  2:237622/139‑1 (MQ=255)                                                                           gCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGAt                                      >  2:266579/1‑139 (MQ=255)                                                                                 tggtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGa                                >  1:688633/1‑139 (MQ=255)                                                                                   gtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                          >  2:313255/1‑63 (MQ=255)                                                                                   gtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                          <  1:313255/63‑1 (MQ=255)                                                                                    tgatgaTAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACca                             <  2:19699/139‑1 (MQ=255)                                                                                            aaCACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACaa                     <  2:688633/139‑1 (MQ=255)                                                                                              cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATg                   >  2:1438250/1‑139 (MQ=255)                                                                                                  aGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGTCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAg               >  1:1235755/1‑139 (MQ=255)                                                                                                    aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCg                                                             >  2:529836/1‑91 (MQ=255)                                                                                                    aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCg                                                             <  1:529836/91‑1 (MQ=255)                                                                                                    aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGaa             >  1:924708/1‑139 (MQ=255)                                                                                                               gcactgcaccGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGAt  <  1:927631/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                       GTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGAT  >  NC_000913/4386758‑4387006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑