Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,058	C→T	G331G (GGC→GGT)	nnr →	bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,058	0	C	T	100.0%	168.0 / NA	52	G331G (GGC→GGT)	nnr	bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCC  >  NC_000913/4394923‑4395186                                                                                                                                        |                                                                                                                                  aGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAg                                                                                                                                >  2:380991/1‑139 (MQ=255)  gTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGc                                                                                                                               <  1:380991/139‑1 (MQ=255)    aCTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCaa                                                                                                                             <  1:896863/139‑1 (MQ=255)        aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGt                                                                                                                         >  1:405280/1‑139 (MQ=255)        aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCAGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGt                                                                                                                         <  1:187193/139‑1 (MQ=255)                        ttGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACt                                                                                                        <  2:405280/139‑1 (MQ=255)                                                    aTTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                            <  2:165508/139‑1 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                                                    >  2:46654/1‑139 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg                                                                                                                      >  2:626971/1‑88 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg                                                                                                                      <  1:626971/88‑1 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                                                   <  1:1189208/139‑1 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                                                   >  1:952150/1‑139 (MQ=255)                                                                   aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGcc                                                             >  1:1469607/1‑139 (MQ=255)                                                                    cTGACGATGGACTCTCTTACCGAAAGCTTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAAATTTCGCAAACCGATGTTGTGGGATGCCg                                                            <  1:322079/139‑1 (MQ=255)                                                                    cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                 >  2:291828/1‑134 (MQ=255)                                                                    cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                 <  1:291828/134‑1 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg                                                                                                                      >  1:204798/1‑79 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg                                                                                                                      <  2:204798/79‑1 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt                                                          >  2:102602/1‑139 (MQ=255)                                                                            ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTg                                                    >  2:79355/1‑139 (MQ=255)                                                                                 ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                                                    >  1:876147/1‑118 (MQ=255)                                                                                 ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                                                    <  2:876147/118‑1 (MQ=255)                                                                                   ctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc                                             >  2:9910/1‑139 (MQ=255)                                                                                          aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                      <  1:102602/139‑1 (MQ=255)                                                                                          aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                      <  1:109001/139‑1 (MQ=255)                                                                                          aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                      >  2:1453138/1‑139 (MQ=255)                                                                                          aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                      >  2:109001/1‑139 (MQ=255)                                                                                            aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGA‑GTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaa                                   >  1:124954/1‑139 (MQ=255)                                                                                                         ccGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                                              >  2:275931/1‑84 (MQ=255)                                                                                                         ccGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                                              <  1:275931/84‑1 (MQ=255)                                                                                                            aTGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTca                    >  1:1007371/1‑139 (MQ=255)                                                                                                             tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg                                           <  2:966455/115‑1 (MQ=255)                                                                                                             tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg                                           >  1:966455/1‑115 (MQ=255)                                                                                                              gtggtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcaca                  <  2:952150/139‑1 (MQ=255)                                                                                                                 gtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg                                                                                                                      <  2:1030165/36‑1 (MQ=255)                                                                                                                 gtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg                                                                                                                      >  1:1030165/1‑36 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                   <  1:320758/65‑1 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                   >  2:320758/1‑65 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGa         >  2:1387225/1‑139 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCCGCTGGCAATCAATCCCGATa                           >  1:124456/1‑121 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCCGCTGGCAATCAATCCCGATa                           <  2:124456/121‑1 (MQ=255)                                                                                                                         tGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTCGTGGGATGCCGATGCa                                                       <  1:752000/91‑1 (MQ=255)                                                                                                                         tGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTCGTGGGATGCCGATGCa                                                       >  2:752000/1‑91 (MQ=255)                                                                                                                            tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAACAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg    >  1:1042918/1‑139 (MQ=255)                                                                                                                            tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                                             >  2:515481/1‑66 (MQ=255)                                                                                                                            tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                                             <  1:515481/66‑1 (MQ=255)                                                                                                                            tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg    >  1:1028972/1‑139 (MQ=255)                                                                                                                            tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg    >  1:728621/1‑139 (MQ=255)                                                                                                                              ccGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc  >  2:884440/1‑139 (MQ=255)                                                                                                                              ccGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc  <  1:79355/139‑1 (MQ=255)                                                                                                                                 gTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa                                                                               <  2:483733/59‑1 (MQ=255)                                                                                                                                 gTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa                                                                               >  1:483733/1‑59 (MQ=255)                                                                                                                                        |                                                                                                                                  AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCC  >  NC_000913/4394923‑4395186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑