| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,424,386 | G→A | *229* (TAG→TAA) | ulaF → | L‑ribulose 5‑phosphate 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,424,386 | 0 | G | A | 100.0% | 105.3 / NA | 33 | *229* (TAG→TAA) | ulaF | L‑ribulose 5‑phosphate 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/18); total (15/18) | |||||||||||
AAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAG > NC_000913/4424262‑4424519 | aagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCgg > 2:297420/1‑139 (MQ=255) ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct < 2:36591/139‑1 (MQ=255) ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct < 1:987485/139‑1 (MQ=255) cGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCg > 2:565623/1‑139 (MQ=255) ttGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGAt < 1:297420/139‑1 (MQ=255) aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACAcgcg < 2:346036/74‑1 (MQ=255) aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACAcgcg > 1:346036/1‑74 (MQ=255) cTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGccc > 1:271748/1‑139 (MQ=255) cTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTg > 2:765437/1‑139 (MQ=255) gatgaATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGcc < 1:1296977/132‑1 (MQ=255) gatgaATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGcc > 2:1296977/1‑132 (MQ=255) aaaCACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGC‑GAAggggg < 1:765437/139‑1 (MQ=255) acTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAg > 1:1240549/1‑119 (MQ=255) acTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAg < 2:1240549/119‑1 (MQ=255) ttCATGCGTTAACCCCGGCCTAAAGCTTATTTACGGG‑AGAAATAAAACAACCGGTGGCGAAATTTCCTTCCTCGGGAAATAACCGGTCTAATTCCCGCGGCGTTTTTCAAAAAAAAAGCCCCCTGC‑GAAGGGGgcaaag < 1:991809/139‑1 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGa > 1:1099962/1‑59 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGa < 2:1099962/59‑1 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc > 1:557748/1‑82 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc < 2:557748/82‑1 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGc < 1:202288/117‑1 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGc > 2:202288/1‑117 (MQ=255) tCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAACCCCCTTCGGAAGGGGgaaaagc > 2:991809/1‑139 (MQ=255) tGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCt < 1:536866/119‑1 (MQ=255) tGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCt > 2:536866/1‑119 (MQ=255) ggTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAGAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGt > 1:1426991/1‑139 (MQ=255) ggTCCTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGt < 2:271748/139‑1 (MQ=255) cTAACGCTTA‑TTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAGAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGTTTCg < 2:1426991/139‑1 (MQ=255) ta‑ttaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc > 1:1201818/1‑56 (MQ=255) ta‑ttaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc < 2:1201818/56‑1 (MQ=255) taCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATAcc < 1:1005743/139‑1 (MQ=255) cGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct < 1:540300/40‑1 (MQ=255) cGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct > 2:540300/1‑40 (MQ=255) aaGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg < 2:1242328/139‑1 (MQ=255) | AAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTA‑TTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGC‑GAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAG > NC_000913/4424262‑4424519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |