Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,442,007	T→C	E57G (GAG→GGG)	msrA ←	methionine sulfoxide reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,442,007	0	T	C	100.0%	190.9 / NA	53	E57G (GAG→GGG)	msrA	methionine sulfoxide reductase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

TCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCG  >  NC_000913/4441872‑4442141                                                                                                                                        |                                                                                                                                       tCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     <  1:869225/139‑1 (MQ=255)  cGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCcac                                                                                                                                    <  1:963380/139‑1 (MQ=255)          aTGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGa                                                                                                                            >  1:373420/1‑139 (MQ=255)           tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACGCCCCAGaa                                                                                                                           >  2:223185/1‑139 (MQ=255)           tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGaa                                                                                                                           >  2:810001/1‑139 (MQ=255)           tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGaa                                                                                                                           >  1:1347420/1‑139 (MQ=255)           tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGaa                                                                                                                           >  2:1241602/1‑139 (MQ=255)            gCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGaaa                                                                                                                          >  2:1288934/1‑139 (MQ=255)                     ttCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATc                                                                                                                 >  2:861006/1‑139 (MQ=255)                     ttCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATc                                                                                                                 >  1:546226/1‑139 (MQ=255)                                ccGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACGCCCCAGAAACAACCCATCGCAAAAATGGc                                                                                                      <  1:223185/139‑1 (MQ=255)                                ccGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGc                                                                                                      <  1:1241602/139‑1 (MQ=255)                                  gTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     <  1:655107/106‑1 (MQ=255)                                  gTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     >  2:655107/1‑106 (MQ=255)                                  gTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCaa                                                                                                    <  1:861006/139‑1 (MQ=255)                                   tATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAAt                                                                                                   >  2:33433/1‑139 (MQ=255)                                   tATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAAt                                                                                                   <  2:546226/139‑1 (MQ=255)                                        ccGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGTCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCa                                                                                              >  1:941464/1‑139 (MQ=255)                                          ggAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCAtt                                                                                            >  1:77569/1‑139 (MQ=255)                                          ggAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCAtt                                                                                            <  1:810001/139‑1 (MQ=255)                                           gAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTc                                                                                           <  2:373420/139‑1 (MQ=255)                                            aGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTcc                                                                                          <  2:47905/139‑1 (MQ=255)                                             gCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     >  1:205784/1‑95 (MQ=255)                                             gCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     <  2:205784/95‑1 (MQ=255)                                                       cGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACAt                                                                               <  1:637399/139‑1 (MQ=255)                                                         aTAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTg                                                                             <  1:33433/139‑1 (MQ=255)                                                           aaGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGt                                                                           >  1:1308147/1‑139 (MQ=255)                                                             gTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     >  2:556888/1‑79 (MQ=255)                                                             gTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     <  1:556888/79‑1 (MQ=255)                                                             gTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCa                                                                         <  1:857935/139‑1 (MQ=255)                                                             gTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCa                                                                         >  1:1289631/1‑139 (MQ=255)                                                                  aTTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAg                                                                    <  2:1289631/139‑1 (MQ=255)                                                                   tttGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     <  1:158607/73‑1 (MQ=255)                                                                   tttGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca                                                                                                                                     >  2:158607/1‑73 (MQ=255)                                                                                 gccgGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACAccc                                                                                                                                <  2:1390378/64‑1 (MQ=255)                                                                                 gccgGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACAccc                                                                                                                                >  1:1390378/1‑64 (MQ=255)                                                                                       ataGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGc                                               <  2:77569/139‑1 (MQ=255)                                                                                               cggcggTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTc                                                                                       <  2:1154169/91‑1 (MQ=255)                                                                                               cggcggTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTc                                                                                       >  1:1154169/1‑91 (MQ=255)                                                                                               cggcggTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTAc                                       >  2:918324/1‑139 (MQ=255)                                                                                               cggcggTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTAc                                       >  2:804193/1‑139 (MQ=255)                                                                                                    gTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCa                                  <  2:1347420/139‑1 (MQ=255)                                                                                                     tGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGt                                                                                        >  2:742739/1‑84 (MQ=255)                                                                                                     tGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGt                                                                                        <  1:742739/84‑1 (MQ=255)                                                                                                         gTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCggg                             <  2:153475/139‑1 (MQ=255)                                                                                                                  gggTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACAt                                                                               >  2:771418/1‑80 (MQ=255)                                                                                                                  gggTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACAt                                                                               <  1:771418/80‑1 (MQ=255)                                                                                                                             aGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGc         <  1:1288934/139‑1 (MQ=255)                                                                                                                                    aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTcc                                                                                          <  1:1469257/51‑1 (MQ=255)                                                                                                                                    aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTcc                                                                                          >  2:1469257/1‑51 (MQ=255)                                                                                                                                    aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGt                                                             >  2:853409/1‑80 (MQ=255)                                                                                                                                    aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATcggcg  >  2:841445/1‑139 (MQ=255)                                                                                                                                    aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCAATGAGTGACGGt                                                             <  1:853409/80‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCG  >  NC_000913/4441872‑4442141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑