Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 177.1 / NA 55*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

CGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGG  >  NC_000913/4490007‑4490260
                                                                                                                                      |                                                                                                                       
ttagccattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                     <  1:537429‑M2/19‑1 (MQ=255)
               agtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGAt                                                                                                      >  2:783242‑M2/106‑139 (MQ=255)
                 taaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                     >  2:302156‑M2/104‑122 (MQ=255)
                 taaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                     <  1:302156‑M2/19‑1 (MQ=255)
                          ttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                           >  1:175863‑M2/95‑139 (MQ=255)
                           tctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAcc                                                                                          >  1:650591‑M2/94‑139 (MQ=255)
                           tctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAcc                                                                                          >  1:156126‑M2/94‑139 (MQ=255)
                             tgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCg                                                                                        <  2:91782‑M2/48‑1 (MQ=255)
                                 tccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                    <  2:175863‑M2/52‑1 (MQ=255)
                                  ccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTc                                                                                   <  2:156126‑M2/53‑1 (MQ=255)
                                      cgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACa                                                                               >  1:338403‑M2/83‑139 (MQ=255)
                                       gtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                              <  2:1113029‑M2/58‑1 (MQ=255)
                                                        gggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCg                                                             <  2:650591‑M2/75‑1 (MQ=255)
                                                         ggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGt                                                            <  2:338403‑M2/76‑1 (MQ=255)
                                                           ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                      >  2:462104‑M2/62‑111 (MQ=255)
                                                           ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                      <  1:462104‑M2/50‑1 (MQ=255)
                                                                  atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                <  2:1235346‑M2/24‑1 (MQ=255)
                                                                  atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                >  1:1235346‑M2/55‑78 (MQ=255)
                                                                          tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                    <  2:1297075‑M2/52‑1 (MQ=255)
                                                                          tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                    >  1:1297075‑M2/47‑98 (MQ=255)
                                                                              ccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCa                                       >  2:1417910‑M2/43‑139 (MQ=255)
                                                                                            ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGcc                         >  1:168343‑M2/29‑139 (MQ=255)
                                                                                              agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                       <  1:1417910‑M2/113‑1 (MQ=255)
                                                                                              agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                       <  2:401384‑M2/113‑1 (MQ=255)
                                                                                              agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                       <  1:571492‑M2/113‑1 (MQ=255)
                                                                                                  taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         <  2:1293173‑M2/47‑1 (MQ=255)
                                                                                                  taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         >  1:1293173‑M2/23‑69 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         >  2:219718‑M2/17‑63 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         <  1:219718‑M2/47‑1 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTc                                                                                   >  2:1458267‑M2/17‑69 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTc                                                                                   <  1:1458267‑M2/53‑1 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggtt                                                                    >  2:534241‑M2/17‑84 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggtt                                                                    <  1:534241‑M2/68‑1 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAata             >  1:1349989‑M2/17‑139 (MQ=255)
                                                                                                        acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAata             >  2:1460192‑M2/17‑139 (MQ=255)
                                                                                                         cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         >  2:1364713‑M2/16‑62 (MQ=255)
                                                                                                         cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         <  1:1364713‑M2/47‑1 (MQ=255)
                                                                                                          gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                            <  2:239505‑M2/28‑1 (MQ=255)
                                                                                                          gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                            >  1:239505‑M2/15‑42 (MQ=255)
                                                                                                          gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa                                                                                                     >  2:400729‑M2/15‑49 (MQ=255)
                                                                                                          gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa                                                                                                     <  1:400729‑M2/35‑1 (MQ=255)
                                                                                                          gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                              <  2:418383‑M2/58‑1 (MQ=255)
                                                                                                          gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                              >  1:418383‑M2/15‑72 (MQ=255)
                                                                                                           tccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCa          >  1:1290284‑M2/14‑139 (MQ=255)
                                                                                                             cggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                            <  2:536543‑M2/108‑1 (MQ=255)
                                                                                                             cggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                            >  1:536543‑M2/12‑119 (MQ=255)
                                                                                                              ggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCaaaa       >  1:1057592‑M2/11‑139 (MQ=255)
                                                                                                               gcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                      <  1:1024895‑M2/114‑1 (MQ=255)
                                                                                                               gcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                      >  2:1024895‑M2/10‑123 (MQ=255)
                                                                                                                   agagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACgggg  <  2:1057592‑M2/134‑1 (MQ=255)
                                                                                                                   agagaGAAGAGAAAATAACTAAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACgggg  <  1:1016118‑M2/134‑1 (MQ=255)
                                                                                                                              aaaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCa          <  1:652040/120‑1 (MQ=255)
                                                                                                                              aaaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCa          >  2:652040/1‑120 (MQ=255)
                                                                                                                                   aaCTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                      >  2:124233/1‑103 (MQ=255)
                                                                                                                                   aaCTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                      <  1:124233/103‑1 (MQ=255)
                                                                                                                                      |                                                                                                                       
CGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGG  >  NC_000913/4490007‑4490260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: