Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,504,849	A→G	T264T (ACA→ACG)	yjhB →	putative MFS transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,504,849	0	A	G	100.0%	212.9 / NA	60	T264T (ACA→ACG)	yjhB	putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/32);  total (28/32)

TCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTA  >  NC_000913/4504716‑4504982                                                                                                                                      |                                                                                                                                      tCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGAc                                                                                                                                  <  2:70614/139‑1 (MQ=255)    ttttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAgg                                                                                                                               >  2:192830/1‑139 (MQ=255)        ccTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACa                                                                                                                           <  1:192830/139‑1 (MQ=255)                 tttttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTa                                                                                                                             <  2:1373913/128‑1 (MQ=255)                 tttttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTa                                                                                                                             >  1:1373913/1‑128 (MQ=255)                 tttttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATAtttttt                                                                                                                  >  1:82310/1‑139 (MQ=255)                          aTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                         <  1:378910/139‑1 (MQ=255)                          aTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                         <  2:630058/139‑1 (MQ=255)                           tttGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgttg                                                                                                        <  2:730273/139‑1 (MQ=255)                             tGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGt                                                                                                      <  2:956378/139‑1 (MQ=255)                                     aCTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                                              >  2:1105376/1‑139 (MQ=255)                                                       tactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgt                                                                                                          <  2:1419395/109‑1 (MQ=255)                                                       tactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgt                                                                                                          >  1:1419395/1‑109 (MQ=255)                                                              ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     <  2:1184725/139‑1 (MQ=255)                                                              ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     <  2:82310/139‑1 (MQ=255)                                                              ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     <  1:1234866/139‑1 (MQ=255)                                                              ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     <  2:766105/139‑1 (MQ=255)                                                                   aCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCt                                                                >  1:345456/1‑139 (MQ=255)                                                                       ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCtaata                                                            >  2:726053/1‑139 (MQ=255)                                                                                  gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                                              >  1:551990/1‑94 (MQ=255)                                                                                  gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGCTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                                              <  2:551990/94‑1 (MQ=255)                                                                                   aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCg                                                                    >  1:263388/1‑119 (MQ=255)                                                                                   aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCg                                                                    <  2:263388/119‑1 (MQ=255)                                                                                      tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                                      <  1:865852/114‑1 (MQ=255)                                                                                      tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                                      >  2:865852/1‑114 (MQ=255)                                                                                       aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                                         >  1:1292809/1‑94 (MQ=255)                                                                                       aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                                         <  2:1292809/94‑1 (MQ=255)                                                                                             gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTaaaaaa                                                                                  >  2:160865/1‑95 (MQ=255)                                                                                             gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTaaaaaa                                                                                  <  1:160865/95‑1 (MQ=255)                                                                                             gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTc                                      >  2:919473/1‑139 (MQ=255)                                                                                             gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTc                                      <  1:1105376/139‑1 (MQ=255)                                                                                             gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTc                                      >  1:1394790/1‑139 (MQ=255)                                                                                              tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATAttttt                                                                                                                   <  1:106036/61‑1 (MQ=255)                                                                                              tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATAttttt                                                                                                                   >  2:106036/1‑61 (MQ=255)                                                                                              tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                         >  1:1292827/1‑71 (MQ=255)                                                                                              tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                         <  2:1292827/71‑1 (MQ=255)                                                                                               ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGa                                                                                                    >  1:1461232/1‑75 (MQ=255)                                                                                               ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGa                                                                                                    <  2:1461232/75‑1 (MQ=255)                                                                                               ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTa                                                                        <  1:1279070/103‑1 (MQ=255)                                                                                               ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                    >  2:112084/1‑139 (MQ=255)                                                                                               ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTCTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGACTTTGTa                                                                        >  2:1279070/1‑103 (MQ=255)                                                                                                gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     >  1:1200272/1‑105 (MQ=255)                                                                                                gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     <  2:1200272/105‑1 (MQ=255)                                                                                                 tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctct                                  >  2:635625/1‑139 (MQ=255)                                                                                                   aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTa                                                                                                                             >  2:894883/1‑46 (MQ=255)                                                                                                   aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTa                                                                                                                             <  1:894883/46‑1 (MQ=255)                                                                                                            ctGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGa                                                                                               <  2:1141159/67‑1 (MQ=255)                                                                                                            ctGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGa                                                                                               >  1:1141159/1‑67 (MQ=255)                                                                                                               atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                                      >  1:390437/1‑89 (MQ=255)                                                                                                               atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                                      <  2:390437/89‑1 (MQ=255)                                                                                                               atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                    >  2:779522/1‑139 (MQ=255)                                                                                                                   cAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaa                <  2:590888/139‑1 (MQ=255)                                                                                                                         cAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActc          >  1:719585/1‑139 (MQ=255)                                                                                                                          aGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActct         <  1:779522/139‑1 (MQ=255)                                                                                                                           ggTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActctt        <  1:635625/139‑1 (MQ=255)                                                                                                                              ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     <  1:337188/75‑1 (MQ=255)                                                                                                                              ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                     >  2:337188/1‑75 (MQ=255)                                                                                                                              ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActctt        >  1:825192/1‑136 (MQ=255)                                                                                                                              ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActctt        <  2:825192/136‑1 (MQ=255)                                                                                                                                 ggAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTta  <  1:112084/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTA  >  NC_000913/4504716‑4504982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑