Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,549,000 T→C C65R (TGC→CGC)  fimH → minor component of type 1 fimbriae

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,0000TC100.0% 218.7 / NA 61C65R (TGC→CGC) fimHminor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

GTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAG  >  NC_000913/4548867‑4549112
                                                                                                                                     |                                                                                                                
gTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCAt                                                                                                             <  1:207585/139‑1 (MQ=255)
gTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCAt                                                                                                             >  1:538822/1‑139 (MQ=255)
                       aTGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACa                                                                                     <  1:1203652/139‑1 (MQ=255)
                                   tCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTcac                                                                          >  2:489746/1‑139 (MQ=255)
                                              ggTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGa                                                                                               >  2:90758/1‑107 (MQ=255)
                                              ggTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGa                                                                                               <  1:90758/107‑1 (MQ=255)
                                                tGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACa                                                                                     <  2:726331/115‑1 (MQ=255)
                                                tGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACa                                                                                     >  1:726331/1‑115 (MQ=255)
                                                 ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                            <  1:191088/139‑1 (MQ=255)
                                                 ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACAATGTCACACTGCAACGAGGCt                                                            >  1:634833/1‑139 (MQ=255)
                                                 ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGCAAAAACTGGTTGTGGAACTTTCGACGGCAATCTTTCGGCCAAAAGATTATCCGGGAACCATTACAGAATTTGTTCAACTGCAACCAgggtt                                                           >  2:565711/1‑136 (MQ=255)
                                                   cAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTa                                                                                >  2:375423/1‑117 (MQ=255)
                                                   cAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTa                                                                                <  1:375423/117‑1 (MQ=255)
                                                             gTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                <  2:807135/139‑1 (MQ=255)
                                                                  tGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgtg                                           <  1:1118387/139‑1 (MQ=255)
                                                                     aaaCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCAt                                                                                                             <  2:82252/70‑1 (MQ=255)
                                                                     aaaCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCAt                                                                                                             >  1:82252/1‑70 (MQ=255)
                                                                       aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCAt                                                                                                             <  2:1312214/68‑1 (MQ=255)
                                                                       aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCAt                                                                                                             >  1:1312214/1‑68 (MQ=255)
                                                                       aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATc                                      >  1:1205399/1‑139 (MQ=255)
                                                                       aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATc                                      >  1:572868/1‑139 (MQ=255)
                                                                           tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCg                                                                                                 <  1:35013/76‑1 (MQ=255)
                                                                           tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCg                                                                                                 >  2:35013/1‑76 (MQ=255)
                                                                           tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgt                                            >  2:445840/1‑129 (MQ=255)
                                                                           tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgt                                            <  1:445840/129‑1 (MQ=255)
                                                                            gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATc                                                                                                   <  1:5230/73‑1 (MQ=255)
                                                                            gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATc                                                                                                   >  2:5230/1‑73 (MQ=255)
                                                                            gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGt                                                                             <  1:483460/95‑1 (MQ=255)
                                                                            gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGt                                                                             >  2:483460/1‑95 (MQ=255)
                                                                             cgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                >  1:257455/1‑123 (MQ=255)
                                                                             cgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                <  2:257455/123‑1 (MQ=255)
                                                                             cgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAt                                  >  2:440250/1‑137 (MQ=255)
                                                                             cgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAt                                  <  1:440250/137‑1 (MQ=255)
                                                                                   tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTAAGGCGGCgt                                            <  2:247922/121‑1 (MQ=255)
                                                                                   tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTAAGGCGGCgt                                            >  1:247922/1‑121 (MQ=255)
                                                                                   tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCACCATAACGa                                                                                                        >  2:246370/1‑61 (MQ=255)
                                                                                   tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCACCATAACGa                                                                                                        <  1:246370/61‑1 (MQ=255)
                                                                                     gtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                        <  2:1205399/139‑1 (MQ=255)
                                                                                     gtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                        <  1:1355256/139‑1 (MQ=255)
                                                                                     gtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACAATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                        <  2:634833/139‑1 (MQ=255)
                                                                                             ggggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                            <  2:772784/95‑1 (MQ=255)
                                                                                             ggggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                            >  1:772784/1‑95 (MQ=255)
                                                                                              gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACa                                                                                     >  2:576523/1‑69 (MQ=255)
                                                                                              gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACa                                                                                     <  1:576523/69‑1 (MQ=255)
                                                                                              gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat               >  2:368943/1‑139 (MQ=255)
                                                                                              gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat               >  1:1362639/1‑139 (MQ=255)
                                                                                               ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCtt                                                      <  1:1068090/99‑1 (MQ=255)
                                                                                               ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCtt                                                      >  2:1068090/1‑99 (MQ=255)
                                                                                               ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                   <  2:381498/134‑1 (MQ=255)
                                                                                               ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                   >  1:381498/1‑134 (MQ=255)
                                                                                                 cAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAAtata            <  2:1362639/139‑1 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGa                                                                                               <  1:1282342/53‑1 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGa                                                                                               >  2:1282342/1‑53 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgtg                                           >  2:512954/1‑105 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgtg                                           <  1:512954/105‑1 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                        >  1:820411/1‑124 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                        <  2:820411/124‑1 (MQ=255)
                                                                                                    aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTg         >  2:1180190/1‑139 (MQ=255)
                                                                                                      ccTGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGc       >  2:877627/1‑139 (MQ=255)
                                                                                                        tGGTCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg     <  2:572868/139‑1 (MQ=255)
                                                                                                           tCGTGGATCTTTCGACGCAAATCTTTCGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg  <  2:538822/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                
GTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAG  >  NC_000913/4548867‑4549112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: