Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,577,958	C→T	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC ← mcrB ←	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,577,958	0	C	T	100.0%	278.9 / NA	85	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC mcrB	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/36);  total (49/36)

ATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577823‑4578091                                                                                                                                        |                                                                                                                                      atacatacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTCTTCCATTAt                                                                                                                                    >  2:940168/1‑139 (MQ=255)    catacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:1117116/139‑1 (MQ=255)     atacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGt                                                                                                                                >  2:308134/1‑139 (MQ=255)      tacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                               <  2:1227503/139‑1 (MQ=255)       acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                               <  2:1199537/138‑1 (MQ=255)       acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                               >  1:1199537/1‑138 (MQ=255)                 atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAAtt                                                                                                                    >  2:1172196/1‑139 (MQ=255)                       aagaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTgg                                                                                                              >  1:1193763/1‑139 (MQ=255)                        agaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  1:622092/1‑119 (MQ=255)                        agaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  2:622092/119‑1 (MQ=255)                              ttACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATCTTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       >  1:932802/1‑139 (MQ=255)                                aCCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     <  2:932802/139‑1 (MQ=255)                                   gggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  2:528328/139‑1 (MQ=255)                                   gggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:308134/139‑1 (MQ=255)                                             tCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAggg                                                                                        <  1:1172196/139‑1 (MQ=255)                                               aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  1:1350849/1‑96 (MQ=255)                                               aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  2:1350849/96‑1 (MQ=255)                                                     tGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  >  2:544201/1‑121 (MQ=255)                                                     tGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:544201/121‑1 (MQ=255)                                                                  tCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttcttc                                                                   <  1:940168/139‑1 (MQ=255)                                                                                 cATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  2:466105/1‑62 (MQ=255)                                                                                 cATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:466105/62‑1 (MQ=255)                                                                                 cATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGat                                                    >  2:327184/1‑139 (MQ=255)                                                                                   tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  1:1157274/1‑60 (MQ=255)                                                                                   tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  2:1157274/60‑1 (MQ=255)                                                                                   tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGatat                                                  >  2:658949/1‑139 (MQ=255)                                                                                         ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtca                                            >  2:671791/1‑139 (MQ=255)                                                                                          gTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  2:279606/1‑53 (MQ=255)                                                                                          gTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:279606/53‑1 (MQ=255)                                                                                             aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATcac                                        >  1:134706/1‑139 (MQ=255)                                                                                                  gTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       >  2:1022031/1‑71 (MQ=255)                                                                                                  gTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       <  1:1022031/71‑1 (MQ=255)                                                                                                    aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcat                                           >  1:736254/1‑129 (MQ=255)                                                                                                    aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcat                                           <  2:736254/129‑1 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            >  1:426194/1‑59 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  2:426194/59‑1 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       <  1:1097554/64‑1 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       >  2:1097554/1‑64 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:1088931/69‑1 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  >  2:1088931/1‑69 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTta                                                                                             >  1:818626/1‑74 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTta                                                                                             <  2:818626/74‑1 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAg                                                                                          <  1:1073690/77‑1 (MQ=255)                                                                                                         atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAg                                                                                          >  2:1073690/1‑77 (MQ=255)                                                                                                          tatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  2:160053/58‑1 (MQ=255)                                                                                                          tatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            >  1:160053/1‑58 (MQ=255)                                                                                                            ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCa                         >  2:68550/1‑139 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 >  2:846328/1‑50 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  1:846328/50‑1 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  1:1405318/55‑1 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            >  2:1405318/1‑55 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTGATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACATTTTCATTAAGCCAt                        >  2:52404/1‑139 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttc                                                                      >  1:1018486/1‑93 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttc                                                                      <  2:1018486/93‑1 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCAtt                               >  2:537065/1‑132 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCAtt                               <  1:537065/132‑1 (MQ=255)                                                                                                             tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCAt                        >  2:788644/1‑139 (MQ=255)                                                                                                               cGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGatat                                                  <  1:640183/111‑1 (MQ=255)                                                                                                               cGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGatat                                                  >  2:640183/1‑111 (MQ=255)                                                                                                                  aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTtata                                                                                           <  2:1340086/67‑1 (MQ=255)                                                                                                                  aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTtata                                                                                           >  1:1340086/1‑67 (MQ=255)                                                                                                                  aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 >  1:636985/1‑77 (MQ=255)                                                                                                                  aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 <  2:636985/77‑1 (MQ=255)                                                                                                                    aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     <  1:904092/55‑1 (MQ=255)                                                                                                                    aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     >  2:904092/1‑55 (MQ=255)                                                                                                                    aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTAt                 >  2:277214/1‑139 (MQ=255)                                                                                                                    aGGTATCACGCGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAACGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTAt                 >  1:128874/1‑139 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  2:1128823/46‑1 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            >  1:1128823/1‑46 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       <  2:1470444/51‑1 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       >  1:1470444/1‑51 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAggg                                                                                        <  2:507665/66‑1 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAggg                                                                                        >  1:507665/1‑66 (MQ=255)                                                                                                                      gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATcc               >  2:461666/1‑139 (MQ=255)                                                                                                                       tATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCTAAGAAat                                                                          >  1:1230859/1‑79 (MQ=255)                                                                                                                       tATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAat                                                                          <  2:1230859/79‑1 (MQ=255)                                                                                                                            cGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGAGTATCCGGagag         >  2:781272/1‑139 (MQ=255)                                                                                                                              ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGt       >  1:1155730/1‑139 (MQ=255)                                                                                                                                cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     >  2:553128/1‑107 (MQ=255)                                                                                                                                cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     <  1:553128/107‑1 (MQ=255)                                                                                                                                cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGc     >  1:623596/1‑139 (MQ=255)                                                                                                                                cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGc     >  2:326319/1‑139 (MQ=255)                                                                                                                                   ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTtata                                                                                           <  2:693155/50‑1 (MQ=255)                                                                                                                                   ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTtata                                                                                           >  1:693155/1‑50 (MQ=255)                                                                                                                                   ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  1:528532/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      ATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577823‑4578091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑