Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	395,245	G→A	A348V (GCG→GTG)	ampH ←	D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase, penicillin‑binding protein, weak beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	395,245	0	G	A	100.0%	197.2 / NA	62	A348V (GCG→GTG)	ampH	D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase, penicillin‑binding protein, weak beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/35);  total (27/35)

GTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACC  >  NC_000913/395110‑395352                                                                                                                                        |                                                                                                            gTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:300822/139‑1 (MQ=255) gTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:680571/139‑1 (MQ=255)   ttGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCa                                                                                                        >  2:525448/1‑139 (MQ=255)          cAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATAttct                                                                                                 <  2:1233624/139‑1 (MQ=255)                    aTCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  2:1457601/139‑1 (MQ=255)                    aTCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  1:1280059/139‑1 (MQ=255)                       cggACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  1:1238345/138‑1 (MQ=255)                       aGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  2:114517/139‑1 (MQ=255)                         gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGc                                                                                            >  2:137543/1‑129 (MQ=255)                         gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGc                                                                                            <  1:137543/129‑1 (MQ=255)                         gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCg                                                                                  >  2:164714/1‑139 (MQ=255)                         gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCg                                                                                  >  1:213400/1‑139 (MQ=255)                           cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  2:458366/132‑1 (MQ=255)                           cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       >  1:458366/1‑132 (MQ=255)                           cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGcc                                                                                >  1:1330469/1‑139 (MQ=255)                               gggATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCatat                                                                            <  1:164714/139‑1 (MQ=255)                                      ccAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAAt                                                                     <  1:525448/139‑1 (MQ=255)                                              ttATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGc                                                             >  1:1471913/1‑139 (MQ=255)                                                aTTCCCGCTTAGCTCGGTCACCAGG‑CATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac                                                          >  1:1125741/1‑139 (MQ=255)                                                 ttCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac                                                          <  2:1330469/139‑1 (MQ=255)                                                    ccGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGGCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCcaccac                                                       <  1:33774/139‑1 (MQ=255)                                                           gCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCat                                                                              <  2:849506/109‑1 (MQ=255)                                                           gCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCCCGTCAAGGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCat                                                                              >  1:849506/1‑109 (MQ=255)                                                                gTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGcca                                                           >  2:1379354/1‑123 (MQ=255)                                                                gTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGcca                                                           <  1:1379354/123‑1 (MQ=255)                                                                           aTTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATcccc                                >  2:462322/1‑139 (MQ=255)                                                                             tGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:1094271/92‑1 (MQ=255)                                                                             tGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:1094271/1‑92 (MQ=255)                                                                               aTGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGATCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGgac                            <  1:179065/139‑1 (MQ=255)                                                                               aTGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGgac                            >  2:6422/1‑139 (MQ=255)                                                                                  ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    >  1:804269/1‑80 (MQ=255)                                                                                  ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  2:804269/80‑1 (MQ=255)                                                                                   cATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcatc                                                                                   >  1:1339335/1‑80 (MQ=255)                                                                                   cATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcatc                                                                                   <  2:1339335/80‑1 (MQ=255)                                                                                     tCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       >  2:319845/1‑74 (MQ=255)                                                                                     tCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  1:319845/74‑1 (MQ=255)                                                                                      cGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCa                                                                               <  1:113976/81‑1 (MQ=255)                                                                                      cGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCa                                                                               >  2:113976/1‑81 (MQ=255)                                                                                            tatTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:849374/77‑1 (MQ=255)                                                                                            tatTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:849374/1‑77 (MQ=255)                                                                                               ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCCTATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  1:859243/139‑1 (MQ=255)                                                                                               ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  2:1150393/139‑1 (MQ=255)                                                                                               ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  1:6422/139‑1 (MQ=255)                                                                                                tttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGcc           >  2:1386417/1‑139 (MQ=255)                                                                                                 ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:647597/1‑72 (MQ=255)                                                                                                 ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:647597/72‑1 (MQ=255)                                                                                                 ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  2:515734/137‑1 (MQ=255)                                                                                                 ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            >  1:515734/1‑137 (MQ=255)                                                                                                   aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatca                                                                                     >  2:1308581/1‑62 (MQ=255)                                                                                                   aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatca                                                                                     <  1:1308581/62‑1 (MQ=255)                                                                                                   aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGaa                                                                  <  1:1103022/81‑1 (MQ=255)                                                                                                   aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGaa                                                                  >  2:1103022/1‑81 (MQ=255)                                                                                                       cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCAtatata    >  1:1154285/1‑139 (MQ=255)                                                                                                        gcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAc   <  2:1125741/139‑1 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACCAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGGCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  2:1154285/139‑1 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:680068/35‑1 (MQ=38)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          >  2:680068/1‑35 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:1231042/35‑1 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          >  2:1231042/1‑35 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  2:300686/54‑1 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       >  1:300686/1‑54 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  2:213400/139‑1 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  1:529567/139‑1 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  >  2:1475790/1‑139 (MQ=255)                                                                                                         cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  1:820309/139‑1 (MQ=255)                                                                                                                            accaccACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  1:896884/38‑1 (MQ=255)                                                                                                                              caccacAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:776218/43‑1 (MQ=255)                                                                                                                              caccacAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:776218/1‑43 (MQ=255)                                                                                                                                        |                                                                                                            GTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACC  >  NC_000913/395110‑395352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑