| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 49,765 | C→T | intergenic (+134/‑58) | kefC → / → folA | potassium:proton antiporter/dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 49,765 | 0 | C | T | 100.0% | 243.5 / NA | 74 | intergenic (+134/‑58) | kefC/folA | potassium:proton antiporter/dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (40/34); total (40/34) | |||||||||||
GAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGC > NC_000913/49636‑49895 | gagTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGt > 2:1248431/1‑139 (MQ=255) agTGACGTAAATCACCCTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt > 1:981693/1‑139 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCTGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTAGCACCAGTTGGCGACGGtt < 2:981693/139‑1 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt < 1:99910/139‑1 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt > 2:99910/1‑139 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt > 2:1438239/1‑139 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt > 1:963694/1‑139 (MQ=255) agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt > 1:181324/1‑139 (MQ=255) tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa < 2:1114003/139‑1 (MQ=255) tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa < 2:181324/139‑1 (MQ=255) aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg > 2:902366/1‑139 (MQ=255) aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg > 1:179958/1‑139 (MQ=255) aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg > 1:87396/1‑139 (MQ=255) cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc < 2:644735/139‑1 (MQ=255) cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc < 2:617076/139‑1 (MQ=255) cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc < 1:1248431/139‑1 (MQ=255) gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt < 1:1382910/139‑1 (MQ=255) gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt < 1:902366/139‑1 (MQ=255) aTCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTAc > 2:90769/1‑139 (MQ=255) aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtt > 2:1378615/1‑139 (MQ=255) tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc < 2:1444632/113‑1 (MQ=255) tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc > 1:1444632/1‑113 (MQ=255) tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtttt > 1:806159/1‑139 (MQ=255) ttgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 1:659850/1‑139 (MQ=255) gtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa < 1:90769/139‑1 (MQ=255) tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa > 2:460069/1‑139 (MQ=255) gTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCaa < 1:1378615/139‑1 (MQ=255) aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt > 1:1243332/1‑80 (MQ=255) aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt < 2:1243332/80‑1 (MQ=255) gagagaGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggc < 1:460069/139‑1 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc < 2:230526/73‑1 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc > 1:230526/1‑73 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt < 2:659850/139‑1 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc > 1:931542/1‑71 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc < 2:931542/71‑1 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTg > 2:1283639/1‑131 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTg < 1:1283639/131‑1 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTa > 2:862457/1‑139 (MQ=255) gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc > 2:666414/1‑139 (MQ=255) gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc < 2:179958/139‑1 (MQ=255) cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt < 2:910238/71‑1 (MQ=255) cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt > 1:910238/1‑71 (MQ=255) cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa < 1:733216/105‑1 (MQ=255) cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa > 2:733216/1‑105 (MQ=255) cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg > 2:632377/1‑139 (MQ=255) tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGc < 1:35901/83‑1 (MQ=255) tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGc > 2:35901/1‑83 (MQ=255) ggACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa < 2:812587/104‑1 (MQ=255) ggACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa > 1:812587/1‑104 (MQ=255) tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt < 1:698583/64‑1 (MQ=255) tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt > 2:698583/1‑64 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATcgcg > 1:128945/1‑139 (MQ=255) aaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAACCTCAATGAt < 2:1234397/100‑1 (MQ=255) aaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAACCTCAATGAt > 1:1234397/1‑100 (MQ=255) tttCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc > 1:377428/1‑139 (MQ=255) tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc > 2:1430271/1‑41 (MQ=255) tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc < 1:1430271/41‑1 (MQ=255) tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt < 1:80228/42‑1 (MQ=255) tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt > 2:80228/1‑42 (MQ=255) tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt < 2:966937/124‑1 (MQ=255) tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt > 1:966937/1‑124 (MQ=255) acTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc < 1:862457/138‑1 (MQ=255) aaCATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTc > 2:860957/1‑88 (MQ=255) aaCATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTc < 1:860957/88‑1 (MQ=255) catcatCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt < 1:1216762/98‑1 (MQ=255) catcatCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt > 2:1216762/1‑98 (MQ=255) tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGaa > 1:1407666/1‑139 (MQ=255) ccTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt < 2:1033519/75‑1 (MQ=255) ccTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt > 1:1033519/1‑75 (MQ=255) gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa < 2:200597/106‑1 (MQ=255) gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa > 1:944645/1‑106 (MQ=255) gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa < 2:944645/106‑1 (MQ=255) gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa > 1:200597/1‑106 (MQ=255) gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgc > 1:871580/1‑139 (MQ=255) | GAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGC > NC_000913/49636‑49895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |