Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 582,148 Δ1 bp intergenic (+51/+4) tfaD → / ← ybcY pseudogene, DLP12 prophage, tail fiber assembly protein family,Phage or Prophage Related/pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913582,1470G.100.0% 246.8 / NA 56intergenic (+50/+5)tfaD/ybcYpseudogene, DLP12 prophage, tail fiber assembly protein family,Phage or Prophage Related/pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (31/25);  total (31/25)

CTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAG  >  NC_000913/582010‑582272
                                                                                                                                         |                                                                                                                             
cTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTga                                                                                                                              >  1:489963/1‑138 (MQ=255)
                   ttGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAg                                                                                                          <  2:518496/139‑1 (MQ=255)
                          aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                   <  2:489963/139‑1 (MQ=255)
                           ccGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAAc                                                                                                  >  1:151900/1‑139 (MQ=255)
                              tgtTGATACATCAACTGCACGTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGTAAAACact                                                                                               >  2:547123/1‑137 (MQ=255)
                              tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                               >  1:261399/1‑139 (MQ=255)
                                ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAGGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                             >  2:928498/1‑139 (MQ=255)
                                ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                             >  1:345461/1‑139 (MQ=255)
                                ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                             <  2:261399/139‑1 (MQ=255)
                                      cATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGt                                                                                       <  2:345461/139‑1 (MQ=255)
                                        tgaaCGGCACATGAAATCGAGTGGTCTGCGAACCCTGTCAGGGAGTACTCATTGGGAGCATGCCGCAGCACGTCTTAAGCAAGACCATGCTGCGGTTGATGTTATTTTTTTCCTGGAGAGGAAAACATGACGACAGTAc                                                                                     >  2:857734/3‑139 (MQ=255)
                                         cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                    >  1:305140/1‑139 (MQ=255)
                                                 ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                        <  2:853951/111‑1 (MQ=255)
                                                 ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                        >  1:853951/1‑111 (MQ=255)
                                                  cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                     >  2:252700/1‑113 (MQ=255)
                                                  cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                     <  1:252700/113‑1 (MQ=255)
                                                          gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                   <  2:49349/139‑1 (MQ=255)
                                                            gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                         <  1:574183/115‑1 (MQ=255)
                                                            gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                         >  2:574183/1‑115 (MQ=255)
                                                               tccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAATCTTGGTTTTAACATtc                                                              >  1:710149/2‑139 (MQ=255)
                                                               gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                   >  2:295946/1‑102 (MQ=255)
                                                               gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                   <  1:295946/102‑1 (MQ=255)
                                                               gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                     <  1:60570/100‑1 (MQ=255)
                                                               gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                     >  2:60570/1‑100 (MQ=255)
                                                               gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                              <  1:694050/107‑1 (MQ=255)
                                                               gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                              >  2:694050/1‑107 (MQ=255)
                                                                ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                             >  1:161187/1‑139 (MQ=255)
                                                                  tACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCg                                                           >  1:555105/1‑139 (MQ=255)
                                                                     gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                        <  2:161187/139‑1 (MQ=255)
                                                                         ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                           >  1:801879/1‑65 (MQ=255)
                                                                         ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                           <  2:801879/69‑5 (MQ=255)
                                                                         ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                    >  1:644190/1‑139 (MQ=255)
                                                                         ccTGTCAGGGAGTAATCATTGCGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                    <  1:547123/139‑1 (MQ=255)
                                                                          cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGtttt                                                                     <  1:385930/121‑1 (MQ=255)
                                                                          cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGtttt                                                                     >  2:385930/1‑121 (MQ=255)
                                                                            gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                <  2:217325/92‑1 (MQ=255)
                                                                            gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                >  1:217325/1‑92 (MQ=255)
                                                                             tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                <  1:147848/139‑1 (MQ=255)
                                                                             tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                <  1:272122/139‑1 (MQ=255)
                                                                             tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                <  2:352455/139‑1 (MQ=255)
                                                                              cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                  >  1:886396/1‑136 (MQ=255)
                                                                              cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                  <  2:886396/136‑1 (MQ=255)
                                                                                 ggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGt                                            >  2:259520/1‑139 (MQ=255)
                                                                                    gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                         <  1:334511/139‑1 (MQ=255)
                                                                                       aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                      >  1:920964/1‑139 (MQ=255)
                                                                                                    tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGagag                                             <  1:759131/119‑1 (MQ=255)
                                                                                                    tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGagag                                             >  2:759131/1‑119 (MQ=255)
                                                                                                     gCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                  <  1:651684/113‑1 (MQ=255)
                                                                                                     gCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                  >  2:651684/1‑113 (MQ=255)
                                                                                                         cagcaCGTCTTAAGCAAGGACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                  <  2:725405/109‑1 (MQ=255)
                                                                                                         cagcaCGTCTTAAGCAAGGACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                  >  1:725405/1‑109 (MQ=255)
                                                                                                              cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                              <  1:782064/108‑1 (MQ=255)
                                                                                                              cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                              >  2:782064/1‑108 (MQ=255)
                                                                                                              cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt               >  1:438543/1‑139 (MQ=255)
                                                                                                               gTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCtttt              <  2:710149/139‑1 (MQ=255)
                                                                                                                 cTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtgt            >  2:904013/1‑139 (MQ=255)
                                                                                                                         aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa    >  1:459953/1‑139 (MQ=255)
                                                                                                                           aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg  >  2:811530/1‑139 (MQ=255)
                                                                                                                           aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg  >  1:496156/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                             
CTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAG  >  NC_000913/582010‑582272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: