Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,696,452 (A)5→4 intergenic (‑381/+10) uidA ← / ← uidR beta‑D‑glucuronidase/transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,696,4480A.100.0% 147.1 / NA 34intergenic (‑377/+14)uidA/uidRbeta‑D‑glucuronidase/transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/13);  total (21/13)

GGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696317‑1696580
                                                                                                                                   |                                                                                                                                    
ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  1:745529/139‑5 (MQ=255)
  aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:847174/137‑5 (MQ=255)
  aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:847174/1‑133 (MQ=255)
  aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            >  1:473730/1‑139 (MQ=255)
     gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGaa                                                                                                                         >  1:566490/1‑139 (MQ=255)
      aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        >  1:162600/1‑139 (MQ=255)
            tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  1:214274/139‑1 (MQ=255)
            tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  2:473730/139‑1 (MQ=255)
                        tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      >  1:751160/1‑139 (MQ=255)
                               aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaag                                                                                                                                  >  1:658874/1‑104 (MQ=255)
                               aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               >  1:232023/1‑139 (MQ=255)
                               aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATCACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaag                                                                                                                                  <  2:658874/105‑2 (MQ=255)
                                 tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatc                                                                                             >  1:167401/1‑139 (MQ=255)
                                   aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAGGATACCGCCAatcat                                                                                           >  1:20523/1‑139 (MQ=255)
                                   aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                           >  1:170497/1‑139 (MQ=255)
                                       ttttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcgcg                                                                                       <  2:162600/139‑1 (MQ=255)
                                                                               gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCtt                                               <  1:130947/139‑1 (MQ=255)
                                                                                   agagAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGc                                           >  2:887588/1‑139 (MQ=255)
                                                                                          ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  2:232023/139‑1 (MQ=255)
                                                                                              gATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAGGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCgg                                <  2:20523/139‑1 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:347817/139‑1 (MQ=255)
                                                                                                         cgGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGTAAACGACGCTTCAcgc                                                         <  1:564470/103‑1 (MQ=255)
                                                                                                         cgGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGTAAACGACGCTTCAcgc                                                         >  2:564470/1‑103 (MQ=255)
                                                                                                          gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGa                                                                                                                   >  1:771049/1‑44 (MQ=255)
                                                                                                          gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGa                                                                                                                   <  2:771049/44‑1 (MQ=255)
                                                                                                           ggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTa                   >  2:255227/1‑139 (MQ=255)
                                                                                                            gCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGCATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                          >  2:210133/1‑131 (MQ=255)
                                                                                                            gCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                          <  1:210133/131‑1 (MQ=255)
                                                                                                                    aTCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATc          >  2:592049/1‑139 (MQ=255)
                                                                                                                     tCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATcc         <  2:167401/139‑1 (MQ=255)
                                                                                                                          aGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag    >  1:551948/1‑139 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    <  1:430921/57‑1 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    >  2:430921/1‑57 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGcc                              <  1:362452/111‑1 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGcc                              >  2:362452/1‑111 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:595444/1‑139 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:434599/1‑139 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:465923/1‑139 (MQ=255)
                                                                                                                            cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:17782/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
GGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696317‑1696580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: