Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,869,022 (T)6→5 coding (68/1284 nt) yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,869,0170T.100.0% 133.1 / NA 31coding (63/1284 nt)yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/15);  total (16/15)

GAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGA  >  NC_000913/1868887‑1869141
                                                                                                                                  |                                                                                                                            
gAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacgc                                                                                                                      >  1:163813/1‑135 (MQ=255)
           cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          >  2:797681/1‑139 (MQ=255)
           cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          <  1:797681/139‑1 (MQ=255)
                        acacAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGTTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAg                                                                                             >  2:922528/1‑139 (MQ=255)
                               cGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAGAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  2:804030/1‑104 (MQ=255)
                               cGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAGAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:804030/105‑2 (MQ=255)
                                     aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                <  1:725140/139‑1 (MQ=255)
                                        cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  2:319544/1‑95 (MQ=255)
                                        cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:319544/96‑2 (MQ=255)
                                        cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                             >  1:181256/1‑139 (MQ=255)
                                        cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                             >  2:918932/1‑139 (MQ=255)
                                          gTTGGCAAATGTAGTACGGGGGGCATATGACCTGTTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                           <  1:922528/139‑1 (MQ=255)
                                            tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGACAAAAATAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  2:609079/1‑91 (MQ=255)
                                            tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGACAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:609079/92‑2 (MQ=255)
                                              gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                       >  1:427829/1‑139 (MQ=255)
                                                   tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                  >  1:534953/1‑139 (MQ=255)
                                                     tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                >  1:842176/1‑107 (MQ=255)
                                                     tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                <  2:842176/107‑1 (MQ=255)
                                                              gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                <  1:389769/114‑1 (MQ=255)
                                                              gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                >  2:389769/1‑114 (MQ=255)
                                                               ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          <  2:163375/87‑1 (MQ=255)
                                                               ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          >  1:163375/1‑87 (MQ=255)
                                                                 cATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGt                                                    >  2:30116/1‑139 (MQ=255)
                                                                           ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                          <  2:878081/139‑1 (MQ=255)
                                                                                 ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                >  1:653773/1‑95 (MQ=255)
                                                                                 ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                <  2:653773/95‑1 (MQ=255)
                                                                                 ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                    >  1:477899/1‑139 (MQ=255)
                                                                                      cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       >  1:166457/1‑49 (MQ=255)
                                                                                      cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       <  2:166457/52‑4 (MQ=255)
                                                                                         cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                            <  2:181256/139‑1 (MQ=255)
                                                                                             tGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTccc                        <  2:427829/139‑1 (MQ=255)
                                                                                                             aaGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCg        >  2:614334/1‑139 (MQ=255)
                                                                                                                cATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc     <  1:30116/139‑1 (MQ=255)
                                                                                                                   ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                <  2:535686/45‑1 (MQ=255)
                                                                                                                   ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                >  1:535686/1‑45 (MQ=255)
                                                                                                                   ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                              <  2:744176/63‑1 (MQ=255)
                                                                                                                   ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                              >  1:744176/1‑63 (MQ=255)
                                                                                                                   ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa  <  2:163813/139‑1 (MQ=255)
                                                                                                                         tCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                         <  2:289788/94‑1 (MQ=255)
                                                                                                                         tCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                         >  1:289788/1‑94 (MQ=255)
                                                                                                                                  |                                                                                                                            
GAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGA  >  NC_000913/1868887‑1869141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑