| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,614,614 | Δ2 bp | coding (681‑682/849 nt) | focB → | putative formate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,614,613 | 0 | C | . | 94.1% | 60.9 / ‑1.8 | 17 | coding (680/849 nt) | focB | putative formate transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (7/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
| * | NC_000913 | 2,614,614 | 0 | T | . | 100.0% | 69.6 / NA | 17 | coding (681/849 nt) | focB | putative formate transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (7/10); total (7/10) | |||||||||||
TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTG > NC_000913/2614476‑2614737 || ttATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGccccc > 2:798624/1‑138 (MQ=255) ggCGTTCGTTATGCGACAAAATCGTCACCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAACTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCccccccccttct > 1:230065/1‑135 (MQ=255) tcgttATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAg > 2:577061/1‑139 (MQ=255) aCAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGcaca > 1:454486/1‑139 (MQ=255) tCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGa > 1:835810/1‑139 (MQ=255) ccTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGt < 2:454486/139‑1 (MQ=255) cTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGt < 1:798624/139‑1 (MQ=255) cTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCACCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTATCGCAGACAATTTTCCGGCACTGACGGTc < 1:577061/139‑1 (MQ=255) tttGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc < 1:167089/139‑1 (MQ=255) tttGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc < 2:911786/139‑1 (MQ=255) tGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCa > 2:297856/1‑139 (MQ=255) gAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAAt < 1:297856/139‑1 (MQ=255) cTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCt < 1:783273/139‑1 (MQ=255) tGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTc > 2:807143/1‑139 (MQ=255) tCGCCATTTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCgg < 2:464129/139‑1 (MQ=255) aTTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGcccccc > 1:456491/1‑46 (MQ=255) aTTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGcccccc < 2:456491/48‑3 (MQ=255) ttgtttgtGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGCCAATTTTCCGGCCCTGACGGTCAGCCATTTTATTACCGCCAATCCGCTCCCGGTGATGcag > 2:141476/1‑137 (MQ=255) aTTCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTACTCGCAGCTGGCGCACAGTAGCGCAGACCATTTTCCGCCACTGACGGTCGGCCATTTTATTACCGCCAATCTGCTCCAGGTGATGCTGGGTAatagt < 2:230065/139‑3 (MQ=255) ttCCGTTCGCCATTGCCATTCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAAtatta > 2:448213/1‑139 (MQ=255) ccattgccattCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGgcggtg < 1:448213/139‑1 (MQ=255) attCGCCATTTCGCCC‑‑CCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTg < 1:141476/139‑1 (MQ=255) || TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTG > NC_000913/2614476‑2614737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |