Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 247,466 Δ1 bp intergenic (+5/‑171) yafL → / → rayT putative lipoprotein and C40 family peptidase/RAYT REP element‑mobilizing transposase, TnpA(REP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913247,4650G.100.0% 69.6 / NA 17intergenic (+4/‑172)yafL/rayTputative lipoprotein and C40 family peptidase/RAYT REP element‑mobilizing transposase, TnpA(REP)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (8/9);  total (8/9)

TTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCC  >  NC_000913/247329‑247571
                                                                                                                                        |                                                                                                          
tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgat                                                                                                          <  2:650673/139‑3 (MQ=255)
      cGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGt                                                                                                  >  1:903614/1‑139 (MQ=255)
              aaTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTAcgcc                                                                                           <  2:100401/139‑1 (MQ=255)
                                gTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                         <  1:686013/139‑1 (MQ=255)
                                      gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                        >  1:445160/1‑118 (MQ=255)
                                      gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                        <  2:445160/118‑1 (MQ=255)
                                      gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                   >  2:31549/1‑139 (MQ=255)
                                           aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGc                                                                        >  1:764787/1‑129 (MQ=255)
                                           aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGc                                                                        <  2:764787/129‑1 (MQ=255)
                                                       aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGCTTTTGACGGAAGAGACGATTTAGTGAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCGGATGCGACGCTGgc                                                  >  1:569933/1‑139 (MQ=38)
                                                       aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                  <  2:441495/139‑1 (MQ=255)
                                                       aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                  <  2:745430/139‑1 (MQ=255)
                                                             ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                            <  1:512336/123‑1 (MQ=255)
                                                             ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                            >  2:512336/1‑123 (MQ=255)
                                                              tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                   >  1:789258/1‑99 (MQ=255)
                                                              tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                   <  2:789258/99‑1 (MQ=255)
                                                                                                  cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGGTGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc       >  2:778572/1‑139 (MQ=255)
                                                                                                       aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAATTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc  <  2:569933/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                          
TTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCC  >  NC_000913/247329‑247571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: