Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 321,564 | (G)7→6 | intergenic (+483/‑44) | rclR → / → ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 321,558 | 0 | G | . | 97.5% | 163.5 / ‑2.6 | 40 | intergenic (+477/‑50) | rclR/ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (19/20); minor base A (1/0); total (20/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACT > NC_000913/321426‑321671 | cGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggggga > 2:298222/1‑138 (MQ=255) gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACAt < 1:644247/139‑1 (MQ=255) aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCgg > 1:22312/1‑139 (MQ=255) ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 2:52907/139‑1 (MQ=255) ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 1:497551/139‑1 (MQ=255) aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 1:298222/139‑1 (MQ=255) aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTCGTATGTTGCTAGCCAATTAAAAAAGAACGCCCTATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 2:604759/139‑1 (MQ=255) tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt < 2:226130/139‑1 (MQ=255) aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt < 1:399250/139‑1 (MQ=255) gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg < 2:654354/67‑1 (MQ=255) gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg > 1:654354/1‑67 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAGGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAtt > 2:657902/1‑139 (MQ=255) ggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTg > 1:359315/1‑139 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 2:622886/94‑1 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg > 1:625622/1‑139 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATAATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 1:622886/1‑94 (MQ=255) ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 1:192441/89‑1 (MQ=255) ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 2:192441/1‑89 (MQ=255) cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 2:12573/1‑78 (MQ=255) cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 1:12573/78‑1 (MQ=255) cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc > 2:726175/1‑139 (MQ=255) cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc > 1:127042/1‑139 (MQ=255) cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc > 1:360925/1‑139 (MQ=255) cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGG‑AGGGGGGAAACATCCGGCATAAATGGGCATGAAGTAATGGAGTATTAGTCGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACAccc > 2:784900/1‑139 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg > 2:874581/1‑139 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 1:328222/1‑75 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 2:720190/75‑1 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 2:328222/75‑1 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 1:720190/1‑75 (MQ=255) ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 1:675684/79‑1 (MQ=255) ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt > 2:675684/1‑79 (MQ=255) gAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGc < 2:359315/139‑1 (MQ=255) tatttattGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt < 2:752356/139‑1 (MQ=255) tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg < 1:782982/107‑1 (MQ=255) tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg > 2:782982/1‑107 (MQ=255) gattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 1:149668/43‑1 (MQ=255) gattgatCCCCCGGT‑GGGGGGAAACAGCGGGCATAAATGGGCa > 2:149668/1‑43 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg > 2:138709/1‑44 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg < 1:138709/44‑1 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc < 1:219406/86‑1 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc > 2:219406/1‑86 (MQ=255) ccccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg < 1:844403/46‑1 (MQ=255) ccccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg > 2:844403/1‑46 (MQ=255) | CGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACT > NC_000913/321426‑321671 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |