Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 321,564 (G)7→6 intergenic (+483/‑44) rclR → / → ykgE reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913321,5580G.97.5% 163.5 / ‑2.6 40intergenic (+477/‑50)rclR/ykgEreactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (19/20);  minor base A (1/0);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACT  >  NC_000913/321426‑321671
                                                                                                                                    |                                                                                                                 
cGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggggga                                                                                                             >  2:298222/1‑138 (MQ=255)
     gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACAt                                                                                                       <  1:644247/139‑1 (MQ=255)
        aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCgg                                                                                                    >  1:22312/1‑139 (MQ=255)
                     ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       <  2:52907/139‑1 (MQ=255)
                     ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       <  1:497551/139‑1 (MQ=255)
                              aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                                                              <  1:298222/139‑1 (MQ=255)
                              aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTCGTATGTTGCTAGCCAATTAAAAAAGAACGCCCTATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                                                              <  2:604759/139‑1 (MQ=255)
                                   tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                                                         <  2:226130/139‑1 (MQ=255)
                                                            aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                <  1:399250/139‑1 (MQ=255)
                                                                  gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                                                                   <  2:654354/67‑1 (MQ=255)
                                                                  gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                                                                   >  1:654354/1‑67 (MQ=255)
                                                                        tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAGGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAtt                                    >  2:657902/1‑139 (MQ=255)
                                                                         ggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTg                                   >  1:359315/1‑139 (MQ=255)
                                                                          gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                                                               <  2:622886/94‑1 (MQ=255)
                                                                          gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                  >  1:625622/1‑139 (MQ=255)
                                                                          gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATAATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                                                               >  1:622886/1‑94 (MQ=255)
                                                                               ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                                                               <  1:192441/89‑1 (MQ=255)
                                                                               ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                                                               >  2:192441/1‑89 (MQ=255)
                                                                                  cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       >  2:12573/1‑78 (MQ=255)
                                                                                  cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       <  1:12573/78‑1 (MQ=255)
                                                                                  cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                          >  2:726175/1‑139 (MQ=255)
                                                                                  cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                          >  1:127042/1‑139 (MQ=255)
                                                                                  cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                          >  1:360925/1‑139 (MQ=255)
                                                                                  cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGAGGGGGGAAACATCCGGCATAAATGGGCATGAAGTAATGGAGTATTAGTCGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACAccc                          >  2:784900/1‑139 (MQ=255)
                                                                                    aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                        >  2:874581/1‑139 (MQ=255)
                                                                                     gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       >  1:328222/1‑75 (MQ=255)
                                                                                     gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       <  2:720190/75‑1 (MQ=255)
                                                                                     gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       <  2:328222/75‑1 (MQ=255)
                                                                                     gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       >  1:720190/1‑75 (MQ=255)
                                                                                          ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                                                              <  1:675684/79‑1 (MQ=255)
                                                                                          ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                                                              >  2:675684/1‑79 (MQ=255)
                                                                                                  gAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGc          <  2:359315/139‑1 (MQ=255)
                                                                                                          tatttattGATGATTGATCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt  <  2:752356/139‑1 (MQ=255)
                                                                                                                    tgattgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                        <  1:782982/107‑1 (MQ=255)
                                                                                                                    tgattgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                        >  2:782982/1‑107 (MQ=255)
                                                                                                                     gattgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCa                                                                                       <  1:149668/43‑1 (MQ=255)
                                                                                                                     gattgatCCCCCGGTGGGGGGAAACAGCGGGCATAAATGGGCa                                                                                       >  2:149668/1‑43 (MQ=255)
                                                                                                                      attgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATg                                                                                     >  2:138709/1‑44 (MQ=255)
                                                                                                                      attgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATg                                                                                     <  1:138709/44‑1 (MQ=255)
                                                                                                                      attgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                           <  1:219406/86‑1 (MQ=255)
                                                                                                                      attgatCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                           >  2:219406/1‑86 (MQ=255)
                                                                                                                             ccccGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                            <  1:844403/46‑1 (MQ=255)
                                                                                                                             ccccGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                            >  2:844403/1‑46 (MQ=255)
                                                                                                                                    |                                                                                                                 
CGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACT  >  NC_000913/321426‑321671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: