| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 7,925 | +A | coding (35/1431 nt) | yaaJ ← | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 7,922 | 1 | . | A | 97.1% | 108.4 / ‑3.5 | 35 | coding (38/1431 nt) | yaaJ | putative transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (16/18); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CC‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCT > NC_000913/7793‑8055 | ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg > 1:656030/1‑139 (MQ=255) ttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CC‑aaaagga > 1:17595/1‑109 (MQ=255) ttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CC‑aaaagga < 2:17595/113‑5 (MQ=255) gCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCGGATAAATCATTACCGATCCTCA‑AAAGGACGCTGTTAATGATGGAGAAAAAATCTGGCATGAATATACCTCTTAt > 2:434629/1‑139 (MQ=255) gCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTAt > 1:735251/1‑139 (MQ=255) cGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAAt < 1:42701/116‑1 (MQ=255) cGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAAt > 2:42701/1‑116 (MQ=255) cGAAACTGCACAAATCCGGTGCGAAAAGTGAACTAACAACCTGAGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg < 2:573106/85‑1 (MQ=255) cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg > 1:573106/1‑85 (MQ=255) gAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCTCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgcg < 1:434629/139‑1 (MQ=255) gCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACt > 2:438180/1‑139 (MQ=255) caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc < 1:689698/139‑1 (MQ=255) aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg > 1:709227/1‑72 (MQ=255) aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg < 2:709227/72‑1 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgca < 2:175805/90‑1 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgca < 2:649764/90‑1 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgca > 1:175805/1‑90 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgca > 1:649764/1‑90 (MQ=255) gTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAATGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCa < 2:207685/139‑1 (MQ=255) caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa < 2:463490/139‑1 (MQ=255) acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg < 1:344902/47‑1 (MQ=39) acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACg > 2:344902/1‑47 (MQ=39) cgcCGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtat < 1:438180/139‑1 (MQ=255) ccGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtatat < 2:541093/139‑1 (MQ=255) ccGAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtatat > 1:541093/1‑139 (MQ=255) gAAGAGCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATAc > 2:473464/1‑139 (MQ=255) agagCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc < 1:569408/100‑1 (MQ=255) agagCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc > 2:569408/1‑100 (MQ=255) gagCAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCt < 2:656030/139‑1 (MQ=255) cAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg > 2:294826/1‑81 (MQ=255) cAGGTAAATCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg < 1:294826/81‑1 (MQ=255) aaaTCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATc < 1:124145/63‑1 (MQ=255) aaaTCATTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATc > 2:124145/1‑63 (MQ=255) aTTACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGa < 1:473464/139‑1 (MQ=255) ttACCGATCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt < 1:252660/139‑1 (MQ=255) atccc‑ca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg < 2:382418/61‑1 (MQ=255) tccc‑ca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCt > 2:5318/3‑139 (MQ=255) aTCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg > 1:382418/1‑64 (MQ=255) aTCC‑CCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc > 2:767361/1‑139 (MQ=255) ccc‑ca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa < 2:198291/68‑1 (MQ=255) | CCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCC‑CC‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCT > NC_000913/7793‑8055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |