Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,518	(C)6→7	intergenic (+360/‑251)	nth → / → dtpA	DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,512	1	.	C	100.0%	158.9 / NA	45	intergenic (+354/‑257)	nth/dtpA	DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/24);  total (21/24)

AATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAA  >  NC_000913/1712386‑1712632                                                                                                                                |                                                                                                                         aaTCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTca                                                                                                               >  2:522474/1‑139 (MQ=255)    cATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTcagtg                                                                                                            <  1:792202/139‑1 (MQ=255)    cATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTcagtg                                                                                                            <  2:782112/139‑1 (MQ=255)                     gCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGcc                                                                                           >  1:262363/1‑139 (MQ=255)                                            cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTaa                                                                    <  1:522474/139‑1 (MQ=255)                                                 cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTg                                                               >  1:364859/1‑139 (MQ=255)                                                      gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTaa                                                                    >  1:290009/1‑129 (MQ=255)                                                      gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTaa                                                                    <  2:290009/129‑1 (MQ=255)                                                           ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑ccccc                                                                                                                     <  1:502542/74‑1 (MQ=255)                                                           ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑ccccc                                                                                                                     >  2:502542/1‑74 (MQ=255)                                                           ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑cc                                                                                                                        <  2:621478/71‑1 (MQ=255)                                                           ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑cc                                                                                                                        >  1:621478/1‑71 (MQ=255)                                                                aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑ccccc                                                                                                                     >  2:20392/1‑69 (MQ=255)                                                                aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑ccccc                                                                                                                     <  1:20392/69‑1 (MQ=255)                                                                aGAAACTGGACTATCACACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                                                >  2:620325/1‑139 (MQ=255)                                                                        gACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                                        >  2:930253/1‑123 (MQ=255)                                                                        gACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                                        <  1:930253/123‑1 (MQ=255)                                                                             tctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAGTCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTcc                                                   >  2:668088/1‑123 (MQ=255)                                                                             tctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTcc                                                   <  1:668088/123‑1 (MQ=255)                                                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  <  2:99947/105‑1 (MQ=255)                                                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  >  1:99947/1‑105 (MQ=255)                                                                                  cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc                              <  2:364859/139‑1 (MQ=255)                                                                                    aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGGATGGTTGAATTCCCCGCCt                            <  1:620325/139‑1 (MQ=255)                                                                                    aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt                            <  1:777037/139‑1 (MQ=255)                                                                                    aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt                            <  1:386882/139‑1 (MQ=255)                                                                                     gTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTc                           >  2:6726/1‑139 (MQ=255)                                                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  >  2:674585/1‑98 (MQ=255)                                                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  <  1:674585/98‑1 (MQ=255)                                                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                                                         >  2:699592/1‑107 (MQ=255)                                                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                                                         <  1:699592/107‑1 (MQ=255)                                                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAg                         <  2:798789/139‑1 (MQ=255)                                                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAg                         >  1:798789/1‑139 (MQ=255)                                                                                        aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGcc                             <  2:826088/134‑1 (MQ=255)                                                                                        aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGcc                             >  1:826088/1‑134 (MQ=255)                                                                                           aCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  <  1:678072/94‑1 (MQ=255)                                                                                           aCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  >  2:678072/1‑94 (MQ=255)                                                                                             gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTatat                   >  1:319748/1‑139 (MQ=255)                                                                                               tAAATAGCAGAACATATCGCCGTTAAGCCATCA‑ccccccctct                                                                                                                >  2:97453/1‑39 (MQ=255)                                                                                               tAAATAGCAGAACATATCGCCGTTAAGCCATCA‑ccccccctct                                                                                                                <  1:97453/43‑5 (MQ=255)                                                                                                gaaTAGCAGAACATATCGCCGTTAAGCCATCA‑ccc                                                                                                                       <  2:193271/34‑1 (MQ=255)                                                                                                gaaTAGCAGAACATATCGCCGTTAAGCCATCA‑ccc                                                                                                                       >  1:193271/2‑35 (MQ=255)                                                                                                   tAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACa             <  1:331873/139‑1 (MQ=255)                                                                                                   tAGCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACa             <  2:554097/139‑1 (MQ=255)                                                                                                     gCAGAACATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGa           <  1:6726/139‑1 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  >  1:476326/1‑78 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                                  <  2:476326/78‑1 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTccc                                 >  1:731213/1‑111 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTccc                                 <  2:731213/111‑1 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc                              <  2:717109/114‑1 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc                              >  1:717109/1‑114 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt                            >  2:76575/1‑116 (MQ=255)                                                                                                           cATATCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt                            <  1:76575/116‑1 (MQ=255)                                                                                                              atCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTg                                                                                             <  2:776868/48‑1 (MQ=39)                                                                                                              atCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTg                                                                                             >  1:776868/1‑48 (MQ=255)                                                                                                              atCGCCGTTAAGCCATCACCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACaa  >  2:499626/1‑139 (MQ=255)                                                                                                                                |                                                                                                                         AATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAA  >  NC_000913/1712386‑1712632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑