Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,754	(C)5→6	coding (915/1041 nt)	galS ←	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,749	1	.	C	100.0%	183.4 / NA	52	A307G (GCA→GGA)	galS	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/32);  total (20/32)

ATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAG  >  NC_000913/2240617‑2240868                                                                                                                                      |                                                                                                                        aTTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccc                                                                                                                   >  1:412621/1‑138 (MQ=255)    gAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccctgc                                                                                                                <  2:20967/139‑5 (MQ=255)           gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCa                                                                                                          >  1:15673/1‑139 (MQ=255)           gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCa                                                                                                          >  1:816756/1‑139 (MQ=255)                    ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTgg                                                                                                 >  2:520511/1‑139 (MQ=255)                        aTTTGTTGAGTTAGTGATCACCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGTCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTaa                                                                                             >  2:671340/1‑139 (MQ=255)                                    aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                 <  1:277629/139‑1 (MQ=255)                                       gATCGCCGCCGCATTCTGGCGTATTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGc                                                                              >  1:816170/1‑139 (MQ=255)                                        aTCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCa                                                                             <  1:520511/139‑1 (MQ=255)                                         tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGTTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                            <  2:743671/139‑1 (MQ=255)                                         tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                            <  2:923289/139‑1 (MQ=255)                                         tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                            <  2:461818/139‑1 (MQ=255)                                          cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                 >  1:779999/1‑133 (MQ=255)                                          cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                 <  2:779999/133‑1 (MQ=255)                                                cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                  >  1:579025/1‑126 (MQ=255)                                                cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                  <  2:579025/126‑1 (MQ=255)                                                     tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                <  1:802162/139‑1 (MQ=255)                                                       tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTACGGTGGCTAATTTCGCCATTGAAGCaa                                                                            <  2:554349/125‑1 (MQ=255)                                                       tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGCCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                            >  1:554349/1‑125 (MQ=255)                                                            tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                <  1:204503/132‑1 (MQ=255)                                                            tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                >  2:204503/1‑132 (MQ=255)                                                                    cAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCg                                                 <  1:753542/139‑1 (MQ=255)                                                                      gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg                                               <  2:108433/139‑1 (MQ=255)                                                                        aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc                                             <  2:317634/139‑1 (MQ=255)                                                                                   cTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACggg                                  <  2:711242/139‑1 (MQ=255)                                                                                     aaCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg                                <  2:719509/139‑1 (MQ=255)                                                                                     aaCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg                                <  2:816170/139‑1 (MQ=255)                                                                                     aaCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGCTCGGTGGCTAATTACGCCATTGAAGCAATGGGATATCGCACGGTCGTTAATTGCGGGTTGGTGTAACGGGCg                                <  2:15673/139‑1 (MQ=255)                                                                                       cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                              <  2:816756/139‑1 (MQ=255)                                                                                            gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg                                                                               <  2:211607/85‑1 (MQ=255)                                                                                            gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg                                                                               >  1:211607/1‑85 (MQ=255)                                                                                            gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                >  2:168322/1‑100 (MQ=255)                                                                                            gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                <  1:168322/100‑1 (MQ=255)                                                                                                       tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg                                                                                        >  2:887775/1‑65 (MQ=255)                                                                                                       tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg                                                                                        <  1:887775/65‑1 (MQ=255)                                                                                                        ggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                     <  2:482844/51‑1 (MQ=255)                                                                                                        ggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                     >  1:482844/1‑51 (MQ=255)                                                                                                        ggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                 >  1:132002/1‑135 (MQ=255)                                                                                                        ggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                 <  2:132002/135‑1 (MQ=255)                                                                                                           tggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga          <  2:251576/139‑1 (MQ=255)                                                                                                           tggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga          <  1:671340/139‑1 (MQ=255)                                                                                                            ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgat         >  1:884426/1‑139 (MQ=255)                                                                                                            ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgat         >  1:838866/1‑139 (MQ=255)                                                                                                                   ggATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag  <  2:519775/139‑1 (MQ=255)                                                                                                                   ggATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag  <  1:12404/139‑1 (MQ=255)                                                                                                                   ggATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag  <  2:838866/139‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAt                                                                                    <  1:623811/45‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAt                                                                                    >  2:623811/1‑45 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                            <  1:899472/53‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                            >  2:899472/1‑53 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                >  2:750576/1‑65 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                >  2:708327/1‑65 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                <  1:708327/65‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                <  1:750576/65‑1 (MQ=255)                                                                                                                                 tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                       >  1:586587/1‑56 (MQ=255)                                                                                                                                 tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                       <  2:586587/56‑1 (MQ=255)                                                                                                                                      |                                                                                                                        ATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAG  >  NC_000913/2240617‑2240868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑