Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,600,609 (C)5→6 coding (132/471 nt) bcp → peroxiredoxin, thiol peroxidase, thioredoxin‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,600,6041.C97.0% 110.8 / ‑3.5 33coding (127/471 nt)bcpperoxiredoxin, thiol peroxidase, thioredoxin‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (13/19);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAG  >  NC_000913/2600466‑2600730
                                                                                                                                           |                                                                                                                               
tGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGa                                                                                                                                  >  2:602651/1‑139 (MQ=255)
tGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGa                                                                                                                                  >  1:349116/1‑139 (MQ=255)
     ttAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccc                                                                                                                           <  2:90938/139‑1 (MQ=255)
     ttAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccc                                                                                                                           >  2:230872/1‑139 (MQ=255)
        aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        <  2:851250/139‑4 (MQ=255)
        aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        <  1:230872/139‑4 (MQ=255)
        aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        <  2:531598/139‑4 (MQ=255)
        aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        >  1:830781/1‑136 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTg                                                                                                                      <  1:500695/137‑1 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTg                                                                                                                      >  2:500695/1‑137 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  2:139551/139‑1 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  2:830781/139‑1 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  1:921417/139‑1 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    >  1:7787/1‑139 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  1:602651/139‑1 (MQ=255)
             tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  2:7787/139‑1 (MQ=255)
                  ccACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                               <  1:62154/139‑1 (MQ=255)
                    aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                             <  2:349116/139‑1 (MQ=255)
                    aCTGAAAGACCGTGAAAACGTACCGAGATTTAGCTTTCCGCATCATGACAGAGATAAAAGTAATTTGATCGACTTCCAGGGACAGCGTCTTCTGGTTTATTTATACCCGAGAGCCATGACTGCCCGGCTGTACCGTAcg                                                                                                             <  2:560099/139‑2 (MQ=255)
                       gAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGc                                                                                                          >  2:457806/1‑139 (MQ=255)
                               gTGATATCGCACCGAAATTTAGCTTGCCGAATCAAGACGGAGAACTAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTgctgct                                                                                                  <  2:599433/139‑1 (MQ=255)
                                              aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATgg                                                                                   <  1:457806/139‑1 (MQ=255)
                                              aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        >  1:769471/1‑98 (MQ=255)
                                              aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        <  2:769471/101‑4 (MQ=255)
                                                                          aCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgt                                                       <  2:258386/139‑1 (MQ=255)
                                                                              gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                   >  2:225698/1‑139 (MQ=255)
                                                                                 aaTTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGt                                                >  2:781061/1‑139 (MQ=255)
                                                                                  aTTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTa                                               <  1:715421/139‑1 (MQ=255)
                                                                                                       aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                           >  2:313822/1‑74 (MQ=255)
                                                                                                       aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                           <  1:313822/74‑1 (MQ=255)
                                                                                                       aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaa                          >  1:220502/1‑139 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  1:661196/47‑1 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    >  2:661196/1‑47 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                        >  1:859769/1‑139 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        >  1:218029/1‑39 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccccccgg                                                                                                                        <  2:218029/42‑4 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccc                                                                                                                             >  2:277233/1‑37 (MQ=255)
                                                                                                         cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGAccc                                                                                                                             <  1:277233/37‑1 (MQ=255)
                                                                                                         cGTGTTCTGGATTATTTCTACCCGAAAGACATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCCGGGTATCAGAACCGATAAAACCGaaaaa                        >  2:28026/1‑139 (MQ=255)
                                                                                                                  gtatATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATgg                                                                                   <  1:579174/68‑1 (MQ=255)
                                                                                                                  gTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATgg                                                                                   >  2:579174/1‑71 (MQ=255)
                                                                                                                  gTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc                                          >  1:462650/1‑112 (MQ=255)
                                                                                                                  gTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc                                          <  2:462650/112‑1 (MQ=255)
                                                                                                                   tttATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGtt              <  2:859769/139‑1 (MQ=255)
                                                                                                                            aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                      <  1:401641/90‑1 (MQ=255)
                                                                                                                            aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                      >  2:401641/1‑90 (MQ=255)
                                                                                                                               cGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAag  <  2:220502/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                               
TGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAG  >  NC_000913/2600466‑2600730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: