Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,746	(A)8→9	coding (599/906 nt)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,738	1	.	A	100.0%	85.1 / NA	27	G203C (GGC→TGC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (15/12);  total (15/12)

CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511604‑3511869                                                                                                                                        |                                                                                                                                    cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa                                                                                                                                 >  1:114032/1‑139 (MQ=255)                                  ttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGTGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGa                                                                                                 >  2:363437/1‑139 (MQ=255)                                      gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  1:318362/1‑139 (MQ=255)                                                   aGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                <  2:114032/139‑1 (MQ=255)                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                         >  1:31234/1‑139 (MQ=255)                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaa                                                                                                                                <  2:806825/83‑1 (MQ=255)                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaa                                                                                                                                >  1:806825/1‑83 (MQ=255)                                                          cGCATCGACATCGCCTTTAACAATGCGATGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCCCtt                                                                         >  1:602040/1‑139 (MQ=255)                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                       <  2:130974/139‑1 (MQ=255)                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                          >  2:881788/1‑84 (MQ=255)                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                          <  1:881788/87‑4 (MQ=255)                                                                        cTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                           <  2:318362/139‑1 (MQ=255)                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                          <  1:264621/65‑4 (MQ=255)                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                          >  2:264621/1‑62 (MQ=255)                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                               <  2:704170/139‑1 (MQ=255)                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  1:828073/139‑1 (MQ=255)                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg                        >  1:571734/1‑138 (MQ=255)                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg                        <  2:571734/138‑1 (MQ=255)                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGt                       >  1:485527/1‑139 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               <  1:530103/114‑1 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               >  2:530103/1‑114 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                      >  1:710018/1‑139 (MQ=255)                                                                                                              tCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAg                     >  2:94665/1‑139 (MQ=255)                                                                                                                   tCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  >  2:600782/1‑121 (MQ=255)                                                                                                                   tCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  <  1:600782/121‑1 (MQ=255)                                                                                                                         cacaTCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGccc          <  2:821019/139‑1 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCCCGCGCTTCACGTTTGCGGACTCGCCCTTACGGGAAATCAACTGGTTCTCGCCAAAGTAGGTGTGCGGCCCCAACTCCa  >  2:688850/1‑139 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCAc                                                                      >  1:536287/1‑71 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  2:512531/112‑1 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             >  1:512531/1‑112 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  2:147155/1‑139 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCAAGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCAc                                                                      <  2:536287/71‑1 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:163636/1‑41 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:163636/41‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511604‑3511869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑