Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,518,9580CA32.6% 100.0 / 49.9 46pseudogene (238/504 nt)insB1IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base C (14/17);  new base A (9/6);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATT  >  NC_000913/4518824‑4519095
                                                                                                                                      |                                                                                                                                         
gtgatgtgatTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGc                                                                                                                                       <  1:758971/139‑1 (MQ=255)
      tgatTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACt                                                                                                                                 >  2:860440/1‑139 (MQ=255)
        atTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTgg                                                                                                                               <  2:212910/139‑1 (MQ=255)
           gTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGc                                                                                                                            >  1:249622/1‑139 (MQ=255)
           gTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGc                                                                                                                            >  2:337066/1‑139 (MQ=255)
                               aCAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCg                                                                                                        >  2:822902/1‑139 (MQ=255)
                               aCAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCg                                                                                                        <  2:237287/139‑1 (MQ=255)
                                cAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCgg                                                                                                       <  1:608389/139‑1 (MQ=255)
                                      ggCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTg                                                                                                 <  2:249622/139‑1 (MQ=255)
                                        cTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAg                                                                                               >  2:13016/1‑139 (MQ=255)
                                           cGTCGGTGCTAAATCGCGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTc                                                                                            >  2:622785/1‑139 (MQ=255)
                                           cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTc                                                                                            >  1:191132/1‑139 (MQ=255)
                                           cGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTc                                                                                            >  1:928619/1‑139 (MQ=255)
                                              cGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTg                                                                                         <  2:437843/139‑1 (MQ=255)
                                                       aTCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATg                                                                                <  2:521209/139‑1 (MQ=255)
                                                                 cgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCAGTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                      <  2:533948/139‑1 (MQ=255)
                                                                      tctgTTTTTCCCGTATCACATGATACTGAGCACGTTTTTGCCTCACGTCTTCGTTTAACTCACTCTGGCCACACTTGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGTTCGTGGTATAAATGACGGATGGCTGGCCg                                                                 <  2:527626/138‑1 (MQ=21)
                                                                      gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                 <  2:633017/139‑1 (MQ=255)
                                                                                gcgTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAAt                                                       <  1:681667/139‑1 (MQ=255)
                                                                                       aCAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTg                                               >  2:651238/1‑139 (MQ=11)
                                                                                             tACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                           <  1:128234/90‑1 (MQ=255)
                                                                                             tACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                           >  2:128234/1‑90 (MQ=255)
                                                                                                ggaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAg                                      >  2:828900/5‑139 (MQ=11)
                                                                                                  aggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAAt                                                       <  1:442763/121‑1 (MQ=255)
                                                                                                  aggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAAt                                                       >  2:442763/1‑121 (MQ=255)
                                                                                                     aCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                 >  1:193566/1‑139 (MQ=14)
                                                                                                     aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                      >  1:924215/1‑103 (MQ=255)
                                                                                                     aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                      <  2:924215/103‑1 (MQ=255)
                                                                                                     aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGc                                  >  2:600401/1‑139 (MQ=255)
                                                                                                               cgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGc                       <  1:651238/139‑1 (MQ=14)
                                                                                                               cgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGc                       <  1:828900/139‑1 (MQ=14)
                                                                                                                gcACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                       <  1:860440/139‑1 (MQ=255)
                                                                                                                gcACGTCTTCGGTGAACGCACTCTGACCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                       <  1:600401/139‑1 (MQ=255)
                                                                                                                   cgtattCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                 >  2:42269/5‑93 (MQ=17)
                                                                                                                   cgtattCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                 <  1:42269/89‑1 (MQ=17)
                                                                                                                   cgtattCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGc                   >  1:106543/5‑139 (MQ=14)
                                                                                                                            tGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGAtg                                                                         <  2:444157/76‑1 (MQ=18)
                                                                                                                            tGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGAtg                                                                         >  1:444157/1‑76 (MQ=18)
                                                                                                                                cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAgc       >  2:899625/1‑139 (MQ=255)
                                                                                                                                cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAgc       >  2:612200/1‑139 (MQ=255)
                                                                                                                                cGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                      >  2:509589/1‑75 (MQ=18)
                                                                                                                                cGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                      <  1:509589/75‑1 (MQ=17)
                                                                                                                                cGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAgcg      >  2:407425/1‑139 (MQ=11)
                                                                                                                                  cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                 >  1:299840/1‑78 (MQ=18)
                                                                                                                                  cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                 <  2:299840/78‑1 (MQ=18)
                                                                                                                                   aCTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAACAAGCGTTACACTCAGCGCa    <  2:101868/139‑1 (MQ=255)
                                                                                                                                     tctGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCAtt  <  1:337066/139‑1 (MQ=255)
                                                                                                                                      ctGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                 >  1:833427/1‑107 (MQ=255)
                                                                                                                                      ctGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                 <  2:833427/107‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
GTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATT  >  NC_000913/4518824‑4519095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑