Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 66,447 T→C E35G (GAG→GGG)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091366,4470TC100.0% 148.2 / NA 41E35G (GAG→GGG) araDL‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/19);  total (22/19)

GGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTT  >  NC_000913/66318‑66582
                                                                                                                                 |                                                                                                                                       
ggCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCaa                                                                                                                                >  1:434927/1‑139 (MQ=255)
                gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc                                                                                                                      <  1:138588/133‑1 (MQ=255)
                gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc                                                                                                                      >  2:138588/1‑133 (MQ=255)
                            cTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGt                                                                                                    >  2:237205/1‑139 (MQ=255)
                              tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCaa                                                                                                                                >  1:161037/1‑109 (MQ=255)
                              tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCaa                                                                                                                                <  2:161037/109‑1 (MQ=255)
                                        cACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                    >  2:475875/1‑111 (MQ=255)
                                        cACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                    <  1:475875/111‑1 (MQ=255)
                                             gTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTg                                                                                   >  2:711993/1‑139 (MQ=255)
                                                tCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCa                                                                                >  1:741371/1‑139 (MQ=255)
                                                        aaCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg                                                                        >  2:369279/1‑139 (MQ=255)
                                                         aCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGt                                                                       <  1:366782/139‑1 (MQ=255)
                                                             cGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGc                                                                   <  1:237205/139‑1 (MQ=255)
                                                             cGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGc                                                                   >  1:225678/1‑139 (MQ=255)
                                                                        tCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                                <  2:720209/83‑1 (MQ=255)
                                                                        tCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                                >  1:720209/1‑83 (MQ=255)
                                                                         cAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTg                                                       <  1:239072/139‑1 (MQ=255)
                                                                              gTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGtt                                                  >  2:881350/1‑139 (MQ=255)
                                                                               tCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGttt                                                 >  1:864076/1‑139 (MQ=255)
                                                                                  tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag                                              <  2:24398/139‑1 (MQ=255)
                                                                                   gACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgaga                                             <  1:881350/139‑1 (MQ=255)
                                                                                   gACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgaga                                             <  2:188361/139‑1 (MQ=255)
                                                                                        tGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttc                                        >  2:603996/1‑139 (MQ=255)
                                                                                         gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                                <  2:196794/66‑1 (MQ=255)
                                                                                         gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                                >  1:196794/1‑66 (MQ=255)
                                                                                         gTAATCGACGCAGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttct                                       <  1:603996/139‑1 (MQ=255)
                                                                                           aaTCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTaa                                     <  2:434927/139‑1 (MQ=255)
                                                                                                  gCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTg                              >  2:329413/1‑139 (MQ=255)
                                                                                                   ccGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGa                             <  1:478595/139‑1 (MQ=255)
                                                                                                   ccGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGa                             <  1:369279/139‑1 (MQ=255)
                                                                                                                  acaAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGa              <  2:864076/139‑1 (MQ=255)
                                                                                                                         cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAAc                                    >  2:228567/1‑110 (MQ=255)
                                                                                                                         cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAAc                                    <  1:228567/110‑1 (MQ=255)
                                                                                                                         cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCtt       >  1:910679/1‑139 (MQ=255)
                                                                                                                         cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCtt       >  1:375243/1‑139 (MQ=255)
                                                                                                                          gccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCttt      >  2:419891/1‑139 (MQ=255)
                                                                                                                           ccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAAc                                    <  2:183432/108‑1 (MQ=255)
                                                                                                                           ccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAAc                                    >  1:183432/1‑108 (MQ=255)
                                                                                                                            cgcgCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag                                              <  2:469450/97‑1 (MQ=255)
                                                                                                                            cgcgCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag                                              >  1:469450/1‑97 (MQ=255)
                                                                                                                              cgcCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCtt  >  1:809289/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTT  >  NC_000913/66318‑66582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑