Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 572,454 C→T C29C (TGC→TGT)  ylcH → uncharacterized protein, DLP12 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913572,4540CT100.0% 111.1 / NA 35C29C (TGC→TGT) ylcHuncharacterized protein, DLP12 prophage
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/15);  total (20/15)

GAGCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGCGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGTGCTTAAACCGTGGCGTG  >  NC_000913/572323‑572586
                                                                                                                                   |                                                                                                                                    
gAGCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAgggg                                                                                                                               <  2:69643/139‑1 (MQ=255)
  gCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGaa                                                                                                                             <  2:793989/139‑1 (MQ=255)
           acTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAAcc                                                                                                                    >  2:479227/1‑139 (MQ=255)
            cTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACct                                                                                                                   >  1:888026/1‑139 (MQ=255)
               cGGTGACTTCTGCGCTAAACGGGGACGCTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCtctc                                                                                                                >  2:554387/1‑139 (MQ=255)
                           cgcTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCAt                                                                                                    <  2:506918/139‑1 (MQ=255)
                                     ggACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCACAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACAt                                                                                          >  2:448918/1‑139 (MQ=255)
                                                     aTACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGc                                                                                    <  2:849732/129‑1 (MQ=255)
                                                     aTACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGc                                                                                    >  1:849732/1‑129 (MQ=255)
                                                          aTCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCa                                                                                                     >  2:704757/1‑107 (MQ=255)
                                                          aTCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCa                                                                                                     <  1:704757/107‑1 (MQ=255)
                                                               aaCTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGaa                                                                                                                      <  1:34154/85‑1 (MQ=255)
                                                               aaCTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGaa                                                                                                                      >  2:34154/1‑85 (MQ=255)
                                                                      ttctCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATc                                                         >  2:99066/1‑139 (MQ=255)
                                                                               cacaGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCAt                                                                                                    <  2:265892/87‑1 (MQ=255)
                                                                               cacaGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCAt                                                                                                    >  1:265892/1‑87 (MQ=255)
                                                                                    aTGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGcc                                                    <  2:481789/130‑1 (MQ=255)
                                                                                    aTGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGcc                                                    >  1:481789/1‑130 (MQ=255)
                                                                                    aTGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTAt                                                <  2:335089/134‑1 (MQ=255)
                                                                                    aTGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTAt                                                >  1:335089/1‑134 (MQ=255)
                                                                                       cAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTggagga                                        >  2:730711/1‑139 (MQ=255)
                                                                                              gCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACAt                                                                                          <  1:702440/82‑1 (MQ=255)
                                                                                              gCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACAt                                                                                          >  2:702440/1‑82 (MQ=255)
                                                                                                        ttCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCa                       <  1:479227/139‑1 (MQ=255)
                                                                                                            ttGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGACCCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCacg                     <  2:558074/137‑3 (MQ=255)
                                                                                                            ttGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGt                   >  1:528827/1‑139 (MQ=255)
                                                                                                            ttGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATATGGAGGAGGGCAAATGCTTTCGCATg                     >  1:558074/1‑137 (MQ=255)
                                                                                                                   tAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGTGCTTaaa            >  2:904629/1‑139 (MQ=255)
                                                                                                                    aGCGTTTGACCTCTGTGGAGGGGAAGCGTGATCCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGc                                                                         <  2:708533/77‑1 (MQ=255)
                                                                                                                    aGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACct                                                                                                                   <  1:428507/35‑1 (MQ=255)
                                                                                                                    aGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACct                                                                                                                   >  2:428507/1‑35 (MQ=255)
                                                                                                                    aGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGc                                                                         >  1:708533/1‑77 (MQ=255)
                                                                                                                          tGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATc                                                         <  1:398502/87‑1 (MQ=255)
                                                                                                                          tGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATc                                                         >  2:398502/1‑87 (MQ=255)
                                                                                                                             ccTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGTGCTTAAACCGTGGCGTg  >  1:194110/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
GAGCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGCGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGTGCTTAAACCGTGGCGTG  >  NC_000913/572323‑572586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑