Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	738,853	G→A	*254* (TAG→TAA)	ybfD →	H repeat‑associated putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	738,853	0	G	A	100.0%	138.9 / NA	44	*254* (TAG→TAA)	ybfD	H repeat‑associated putative transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

AGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAGAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTA  >  NC_000913/738747‑738974                                                                                                           |                                                                                                                          aGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACg                                                                                           >  1:211521/1‑139 (MQ=255)     tGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAg                                                                                       <  2:903465/139‑1 (MQ=255)      gACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGt                                                                                      >  2:77969/1‑139 (MQ=255)                  aTTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAg                                                                          <  2:250031/139‑1 (MQ=255)                   ttAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCt                                                                                                                <  2:376263/100‑1 (MQ=255)                   ttAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCt                                                                                                                >  1:376263/1‑100 (MQ=255)                   ttAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGc                                                                         >  2:432644/1‑139 (MQ=255)                                     gCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                               >  1:311741/1‑83 (MQ=255)                                     gCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                               <  2:311741/83‑1 (MQ=255)                                               aaCCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaa                                             <  1:77969/139‑1 (MQ=255)                                                aCCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaac                                            >  2:640128/1‑139 (MQ=255)                                                     tCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCt                                                                                               >  1:564532/1‑83 (MQ=255)                                                     tCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCt                                                                                               <  2:564532/83‑1 (MQ=255)                                                     tCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGAt                                       >  1:849793/1‑139 (MQ=255)                                                      cTTCATATTGTTTGCAATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGAtt                                      <  2:98331/139‑1 (MQ=255)                                                               gTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTTAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAgg                                                                      <  2:199804/98‑1 (MQ=255)                                                               gTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAgg                                                                      >  1:199804/1‑98 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAg                                                                                       >  2:198988/1‑78 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAg                                                                                       <  1:198988/78‑1 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaa                                             <  1:710581/120‑1 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaa                                             >  2:710581/1‑120 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACACCAGGATTAACTTCACAAat                          >  2:643700/1‑139 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAat                          >  2:283123/1‑139 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAat                          >  1:239569/1‑139 (MQ=255)                                                                  tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAat                          >  1:356662/1‑139 (MQ=255)                                                                      aTGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCa                      <  2:829779/139‑1 (MQ=255)                                                                            cTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTtctc                <  2:356662/139‑1 (MQ=255)                                                                              gatgaGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                               <  1:588043/74‑1 (MQ=255)                                                                              gatgaGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                               >  2:588043/1‑74 (MQ=255)                                                                                 gaGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGt           <  1:432644/139‑1 (MQ=255)                                                                                 gaGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGt           <  1:274304/139‑1 (MQ=255)                                                                                  aGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTc          <  1:283123/139‑1 (MQ=255)                                                                                   gCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCt                                                 <  1:227951/99‑1 (MQ=255)                                                                                   gCTTATTGATTTCACGTGTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCt                                                 >  2:227951/1‑99 (MQ=255)                                                                                    cTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACt                                  >  2:419742/1‑113 (MQ=255)                                                                                    cTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACt                                  <  1:419742/113‑1 (MQ=255)                                                                                      tATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTaca      >  2:838419/1‑139 (MQ=255)                                                                                        ttGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACt    <  2:239569/139‑1 (MQ=255)                                                                                        ttGATGTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAATCTACCTCACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCGCAAATATCATTTCTCAACGTCTACACt    <  2:558616/139‑1 (MQ=39)                                                                                         tGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                               <  1:165783/63‑1 (MQ=255)                                                                                         tGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                               >  2:165783/1‑63 (MQ=255)                                                                                          gATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTa  <  2:849793/139‑1 (MQ=255)                                                                                             ttCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCg                                                                <  1:287785/74‑1 (MQ=255)                                                                                             ttCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCg                                                                >  2:287785/1‑74 (MQ=255)                                                                                                           |                                                                                                                          AGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAGAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTA  >  NC_000913/738747‑738974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑