Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	775,120	G→A	A123A (GCG→GCA)	ybgC →	acyl‑CoA thioester hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	775,120	0	G	A	100.0%	117.4 / NA	37	A123A (GCG→GCA)	ybgC	acyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

CAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATT  >  NC_000913/774986‑775254                                                                                                                                       |                                                                                                                                       cAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTc                                                                                                                                    >  1:371038/1‑139 (MQ=255)   gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                  <  1:503881/139‑1 (MQ=255)       gAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGt                                                                                                                              >  1:264450/1‑139 (MQ=255)         aaTAACATCAATGCGTGGCACCTCTTTGGTTTTAACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCt                                                                                                                            >  2:664639/1‑139 (MQ=255)                 cAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                    <  2:264450/139‑1 (MQ=255)                 cAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                    <  1:444692/139‑1 (MQ=255)                   aTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                  <  2:830910/139‑1 (MQ=255)                                                aCGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                  <  1:830771/110‑1 (MQ=255)                                                aCGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                  >  2:830771/1‑110 (MQ=255)                                                        tCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTg                                                                             <  1:664639/139‑1 (MQ=255)                                                           aCGCCGAGAATACTTTGCTGAATGTAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                  >  2:904759/1‑99 (MQ=255)                                                           aCGCCGAGAATACTTTGCTGAATGTAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                  <  1:904759/99‑1 (MQ=255)                                                            cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactg                                                                                                 <  2:126907/115‑1 (MQ=255)                                                            cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactg                                                                                                 >  1:126907/1‑115 (MQ=255)                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                          <  1:509172/121‑1 (MQ=255)                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                          >  2:509172/1‑121 (MQ=255)                                                                         ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                          >  2:829213/1‑109 (MQ=255)                                                                         ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                          <  1:829213/109‑1 (MQ=255)                                                                             tgaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCtt                                                           <  1:123849/136‑1 (MQ=255)                                                                             tgaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCtt                                                           >  2:123849/1‑136 (MQ=255)                                                                                 tgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                   >  2:506007/1‑60 (MQ=255)                                                                                 tgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                   <  1:506007/60‑1 (MQ=255)                                                                                    aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGc                                                                  <  2:15438/122‑1 (MQ=255)                                                                                    aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGc                                                                  >  1:15438/1‑122 (MQ=255)                                                                                      cAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                                >  1:276612/1‑90 (MQ=255)                                                                                      cAGAGGTTCTGGTTGTTTGCGATGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                                <  2:276612/90‑1 (MQ=255)                                                                                                ggTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                            >  1:687834/1‑116 (MQ=255)                                                                                                ggTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                            <  2:687834/116‑1 (MQ=255)                                                                                                     tttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttg                                >  1:404499/1‑139 (MQ=255)                                                                                                           ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGt                          >  2:736349/1‑139 (MQ=255)                                                                                                                          tGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCt           <  2:404499/139‑1 (MQ=255)                                                                                                                             aGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTgg        >  1:313390/1‑139 (MQ=255)                                                                                                                              gCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTa                                                                >  1:108830/1‑82 (MQ=255)                                                                                                                              gCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTa                                                                <  2:108830/82‑1 (MQ=255)                                                                                                                               ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc      <  2:371038/139‑1 (MQ=255)                                                                                                                                cTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGcc     >  2:400650/1‑139 (MQ=255)                                                                                                                                  cGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCat   >  2:50056/1‑139 (MQ=255)                                                                                                                                   gTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCatt  >  1:809859/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       CAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATT  >  NC_000913/774986‑775254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑