Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 72,115 G→A *255* (TAG→TAA yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,1150GA100.0% 71.0 / NA 23*255* (TAG→TAAyabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

GCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGT  >  NC_000913/71991‑72250
                                                                                                                            |                                                                                                                                       
gcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAagag                                                                                                                                      >  1:258447/1‑128 (MQ=255)
gcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAagag                                                                                                                                      <  2:258447/128‑1 (MQ=255)
                        cGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                                   <  2:308359/139‑1 (MQ=255)
                                                            tgtTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTa                                                               >  1:852466/1‑139 (MQ=255)
                                                                aaTTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTc                                                           <  2:852466/139‑1 (MQ=255)
                                                                     gCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATa                                                      >  1:589847/1‑139 (MQ=255)
                                                                      ccACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATaa                                                     >  2:314008/1‑139 (MQ=255)
                                                                          ccGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGTCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCg                                                 >  1:84178/1‑139 (MQ=255)
                                                                                    cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGt                                                                >  2:45327/1‑114 (MQ=255)
                                                                                    cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGt                                                                <  1:45327/114‑1 (MQ=255)
                                                                                          atatCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGTTAGTCTTGTGTGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  1:281828/139‑1 (MQ=255)
                                                                                          atatCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGTGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGATCACGCCGCATCCGa                                 <  2:620519/139‑1 (MQ=255)
                                                                                                atatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGc                           >  1:657476/1‑139 (MQ=255)
                                                                                                   ttttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGcc                                                                                                  >  1:590810/1‑65 (MQ=255)
                                                                                                   ttttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGcc                                                                                                  <  2:590810/65‑1 (MQ=255)
                                                                                                     ttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaaaa                                                                            <  1:496720/85‑1 (MQ=255)
                                                                                                     ttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaaaa                                                                            >  2:496720/1‑85 (MQ=255)
                                                                                                     ttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATatt                      >  1:850841/1‑137 (MQ=255)
                                                                                                          tAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGc                                                                         <  1:822370/83‑1 (MQ=255)
                                                                                                          tAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGc                                                                         >  2:822370/1‑83 (MQ=255)
                                                                                                           aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                <  1:314008/139‑1 (MQ=255)
                                                                                                           aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                <  2:657476/139‑1 (MQ=255)
                                                                                                                      ggTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAAt     >  1:287564/1‑139 (MQ=255)
                                                                                                                        tttAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAg   <  2:589847/139‑1 (MQ=255)
                                                                                                                         ttAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGt  >  1:542647/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                       
GCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGT  >  NC_000913/71991‑72250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: